Re: [ccp4bb] Zn+2 proteases

From personal experience, many of them really do need Zn++ and some of them can get by on Cd++ :) However, Zn++ can often be used in the micromolar range (so essentially quasi-stoichiometric with the enzyme) so it's not as much of a PITA as when you have millimolar quantities. HTH! Artem - Co

Re: [ccp4bb] Zn+2 proteases

Hello Jiri, I wouldn't choose neither of them, but Co(II). NMR spectroscopists have been using Co(II) to probe Zn(II) sites in proteins for ages... Check out Bertini&Luchinat (*DOI: *10.1021/ar00092a002 ), or Vallee&Auld (*DOI: * 10.1021/bi00476a001

[ccp4bb] Zn+2 proteases

Dear All I have a questions about the Zn+2 proteases (Thermolysin based Zn+2 site) As Zinc is trouble metal when it comes to test the activity. Which is better substitute for it. Is MgCl2 or MncCl2 Why one is preferred over the other. best Jiri ##