Re: [ccp4bb] about the "working solution"

2011-04-25 Thread vandana kukshal
Hi i also faced this type of problem with my protein. i used additive screen for getting some thick needles and then with that condition i used seeding approach to get thick single crystal. try to use micro seeding with this . i used micro dilution or streak seeding for this. for seeding try to get

[ccp4bb] about the "working solution"

2011-04-25 Thread xinghua qin
hi,everyone, Sorry for the off-topic question.I got two many crystals in the drop with long thin needle shape and crossed , after optimization, no big change appeared, and I got only a few needle clusters in the drop at most times. which one is better for next optimization step and suitale to be