I would try iodoacetamide.
Sent from my iPhone
> On Feb 25, 2021, at 7:58 PM, David wrote:
>
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> I would like to know how to block activated groups on Thiol-activated
> Sepharose 4B (after coupling a peptide). The immobiliz
I would like to know how to block activated groups on Thiol-activated Sepharose
4B (after coupling a peptide). The immobilized peptide will be used later to
purify/identify which antibody binds to this peptide.
For CNBr-activated sepharose I use Tris or ethanolamine to block the remaining
activ
