Have you looked at a self rotation function? Is there a clear 3-fold axis
perhaps?
Eleanor
On 27 April 2017 at 10:45, Mark J van Raaij wrote:
> Does the self-rotation function suggest presence of NCS axes? If so, this
> may help you figure out the symmetry inside the a.u..
> If you haven't done
Does the self-rotation function suggest presence of NCS axes? If so, this may
help you figure out the symmetry inside the a.u..
If you haven't done so already, try diffracting a crystal at room temperature,
to make sure cryo-protection and freezing did not affect the diffraction.
In any case, at
Dear Jademilson,
At a CCP4 APS workshop a few years ago, one of the
students solved a molecular replacement problem where I think there were close
to 40 copies in the asu. I have to say that many people were surprised by this,
but I think the protein was smaller and
Congratulations!
I think you are now looking for additional crystallographic and
non-crystallographic symmetry, because finding 40 particles in arbitrary
positions and orientations is going to be brutal.
I wouldn’t take the cell and point group assignment from XDS at face value.
Rather I thin
[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Jademilson Santos
Sent: Wednesday, April 26, 2017 3:35 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] large number in ASU
Greetings all,
I am having trouble with a data set and would like to know if somebody can
help. I'm working with a protein of approximate
Greetings all,
I am having trouble with a data set and would like to know if somebody can
help. I'm working with a protein of approximately 50 kDa, which I have
successfully crystallized. The crystals diffracted at a resolution of 3,65
angstroms and upon initial processing using XDS i obtained th