Hi, everyone
I want to do IHC from a 5 year old pap smear slides for pan-keratin stain.
I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph
6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40
minutes. I also used Triton x 100 (0.2%) pretreatment 10 minut
I love my Sequenza. It is all I use. (Have 2 old Ventanas gathering dust)
I only purchased the racks and reuse the coverplates.
I do not work for Thermo either.
Paula Pierce
Excalibur Pathology, Inc.
631 N Broadway Ave.
Moore, OK 73160
405-759-3953
www.excaliburpathology.com
- Original M
Hi Joyce,
We have an Autostainer (Dako) for the majority of our IHC. Often,
however, I use a product from Thermo. The Sequenza IHC system is a bit
bloated. It has a timer and a place to put the reagents ... kinda silly
honestly. But, the Sequenza staining racks with cover plates (rest of the
sy
Hi Angela,
My colleague, Courtney, and I have had a Bond Max on demo for a couple of
months now and generally, we like it. We have also had demos from Dako and Lab
Vision, but we are leaning more towards the Bond. This demo process has
revealed that all the instruments have their strong point
Hi.
I am interested in David's post:
I too wished to change to Prills for weighing out PFA because the usual
crystaline PFA is such a fine powder that it tends to "float" around, when
weighing.
( conforming to Health and Safety recommendations by weighing the latter out
in a fume hood The p
