Yes,
I would also agree that this would probably be the case.
I have not tried to redo the DAB step after counterstaining,
dehydrating, clearing and mounting.
I would expect it not to work.
Though I could easily be corrected.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Labor
Emily:
Although it might seem that the reapplication of a substrate-chromogen
solution?after removal of the coverslip/mounting media and rehydration?should
work, it?very rarely does.? The scientific reason is that, after the specimen
has been dehydrated and coverslipped,?the enzyme label (e.g. p
I do not know much about why expiration dates were started, but I would assume
that fear of being sued has a lot to do with it. A missed diagnosis and a
lawyer could go after the lab, pathologist, doctor, and antibody manufacturer.
A manufacturer would loose a lot more money in a lawsuit than
Prior to purchasing the Artisan we were using the Gomori's One-Step trichrome
with great success. Kit H017 at Polyscientific...www.polyrnd.com
From: [EMAIL PROTECTED] on behalf of Henry, Charlene
Sent: Tue 10/14/2008 6:09 PM
To: histonet@lists.utsouthwestern.edu
it's a good thing you asked.
I let my nails (well not my nails personally, but the specimen nails) soak
in 20% ammonium hydroxide or 20% sodium hydroxide for at least 1 hour
(sodium hydroxide works better, but whatever you can get). Then I rinse the
blocks in running water for 2-3 minutes, then
We are going to discontinue the use of the Ventana Trichrome kit because the
kit expires so quickly and we do not perform enough Trichrome stains to use all
of the reagents. We have not performed a manual Trichrome stain in such a long
time that I was wondering what Trichrome protocol is most la
We are having problems getting nail sections to stick to the slide. They
almost always fall off the slide during H&E staining. We use plus slides and
various adhesives - sta-on, hairspray, etc. with no success.
Is there anybody that can help me by sharing their secret method to get nail
secti
Thank you Charles! I contacted Sakura and someone called me immediately. You
were right; the light had been turned down! It's hard when you don't have a
manual. For future reference, I was told that you have to buy the manual
(@ $125)!
-Original Message-
From: Charles E. Brown, Jr. [m
Probably about the same as a human one...
:)
Claire
From: [EMAIL PROTECTED] on behalf of Houston, Ronald
Sent: Tue 10/14/2008 3:21 PM
To: Gagnon, Eric; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Titration Runs on Ventana Ultra
And how much does t
Hi Everyone:
I am encountering problems with curling whenever I lift the antiroll plate and
attempt to retrieve the section. Often the top portion of the section will
remain flat while the bottom curls. I am sectioning fixed rat brains on a
Vibratome Ultrapro 5000 cyrostat. The brains were plac
And how much does this baby cost?
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Gagnon,
Eric
Sent: Tuesday, October 14, 2008 3:53 PM
To: histonet@li
The new Ventana Ultra allows slides requiring titration/manual application of
antibody to be loaded concurrently with protocols using dispensers. This is
because it is a continuous access system, any slide/any time. This is an
exciting change for those of us who have had to run titrations as s
HI Hazel
Our lab has, in fact, been "LEANED". We were somewhat limited by budget of
course, but we did a fair amount of streamlining. We are at 5 sites in the
city, so each site presented it's own challenges. We are currently in the
process of maintaining our processes.
If you would like
To all:
Would anyone have a manual for the above embedding center? Or could tell us how
to set the temperature on the unit? Our settings got eliminated and we can't
figure out how to re-set them?
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine
True, but unless you have every slide on the run as titration the machine
wont start. That's a good trick if you just have a titration run.
Mark
On Tue, Oct 14, 2008 at 11:37 AM, Sebree Linda A. <[EMAIL PROTECTED]>wrote:
> Yes, you can always run in "manual" modeediting the protocol to
> "ti
Yes, you can always run in "manual" modeediting the protocol to
"titration" rather than "antibody". That does indeed allow you to
manually apply the antibody when the instrument signals its time to do
so.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VA
Thanks for the helpful comments. jane
"Life is short - make haste to be kind"
Dr. Jane Flinn
Director, Undergraduate Neuroscience Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA 22030
Phone: 703-993-4107
Fax: 703-993-1359
- Original Message -
From: Jane M Flinn <[E
Thanks, the student indeed had a super saturated solution, Jane
"Life is short - make haste to be kind"
Dr. Jane Flinn
Director, Undergraduate Neuroscience Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA 22030
Phone: 703-993-4107
Fax: 703-993-1359
- Original Message --
Problem solved. jane
"Life is short - make haste to be kind"
Dr. Jane Flinn
Director, Undergraduate Neuroscience Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA 22030
Phone: 703-993-4107
Fax: 703-993-1359
- Original Message -
From: Jane M Flinn <[EMAIL PROTECTED]>
Are you filtering the solutions before immersing the slides? The sodium
chloride is a saturated solution.
Rena Fail
-- Original message from Jane M Flinn <[EMAIL PROTECTED]>:
--
We are running a congo red stain and have sodium chloride crystals precipataing
> onto
Hi Amy,
I use the Ventana Discovery and I add my antibodies manually as I'm in
research and don't feel like shelling out extra for empty containers to
put my Abs in. Do you have an option on the Benchmark to switch the
primary Ab step to "titration" or something like that (can't remember
what th
We are running a congo red stain and have sodium chloride crystals precipataing
onto the slide. Does anyone know why and how we can fix this? jane
"Life is short - make haste to be kind"
Dr. Jane Flinn
Director, Undergraduate Neuroscience Program
George Mason University, 3F5
4400 University Dr.
Liz, When I do some smaller odd type bone samples, in paraffin, we have found
the cutting pulls the cells out of the nice, lacunae network and the cells sit
on top. It is an artifact we have noted for years. Calcified sectioning might
be better. Vicki
___
Liz,
If your controls are fine but your bone marrows are not, could it be
the decalcification process that is damaging these antigens? Try using
EDTA for decal instead of formic acid.
Just a thought.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen
Here is a link to Henry Ford Hospital (Detroit, MI). They are a LEAN lab and
they are holding a seminar for interested labs. Hope this helps.
http://www.henryford.com/HFProductionSystem
Sharon E. Scalise, HTL (ASCP)
Histology Supervisor
William Beaumont Hospital
Royal Oak, MI 48073
248 89
I'm working with a client who it is growing cells on a demineralized
bone matrix. We have processed the tissue both manually and on a VIP
and we are seeing that the cells are not attached to the bone and that
they are separated away from the bone. We are trying to figure out if
this is a processi
Hello All
I need a bit of help here. We have been trying to localize CD105 and
CD166 in human bone. Our control tissue is staining just fine (human
tonsil and human skin) but we have been unable to demonstrate any of
these cells within the bone marrow. Any help would be appreciated,
especially
Hazel,
I am scheduled to attend a Surgical Pathology Lean training workshop at Henry
Ford Hospital in Detroit. You can see the information at this link
http://www.henryford.com/body.cfm?id=50135
I don't know anyone who works in the lab there but you might find it useful to
contact the histo la
I have not heard from any labs that have gone to the LEAN process. Are
there none out there?
I have heard from labs who are interested in it but not one response
from a lab who is LEAN.
Thanks.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800
We have found that when using the Bond, it is easier to get started with 10
slides, or 1 or 2 small cases. Our pathologists make requests throughout the
day, and to have to hold up the entire run so that another case can be added
creates a delay in results.
-Original Message-
From: [EM
You don't need to decolorize. Remove the coverslip, rehydrate to water (the
eosin will come out in the alcohol), and then begin your IHC procedure.
Hopefully, the tissue is mounted on an adhesive or charged slide. If not, you
may have to cut back your antigen retrieval. Also, you may want to
They are very competent - however, I am hoping to find a vendor in the
interest of time management, as they must currently prepare huge volumes
for literally hundreds of samples per week. It's all about lean
laboratory practices - these are some of the finest histotechnologists in
the world -
32 matches
Mail list logo
