Re: [Histonet] fixative for brains?

Jenee- I have almost always used 10% NBF for brain fixation.  We're talking rats, mice, guinea pigs, hamsters, pigs, dogs, primates, etc. The only exception to that was when we were having an issue with the brains settling to the bottom of the fixation container and developing flat spots where

RE: [Histonet] Acidified Hematoxylin

You might haveto make some in-house. The following is easy to make and can be used soon after preparing. Use a good control (skin, appendix) to control your times: Debidin's Haematoxylin This solution uses sodium hypochlorite as the oxidant in the preparation of alum haematoxylin. This reagent

Fw: Re: [Histonet] Medical Equipment Source

Hi Ann, I also just purchased a Leica XL Autostainer and Leica CV 5000 coverslipper recently from Medical Equipment Source after calling all of the usual companies offering refurbished equipment.  The pricing is a lot less than everyone else with wonderful customer service, fast shipping and  be

Re: [Histonet] Medical Equipment Source

Hi Ann, Our lab purchased a Leica 2030 microtome (using disposable blades)through Medical Equipment Source. I'd looked at several companies that sell refurbished microtomes, and went with this company because of the cost ($3000) that included a warranty and a newer knife holder than the origi

[Histonet] Microtome Knife Sharpner

Dear List, Our lab has American Optical Model 925 and 935 Microtome knife sharpeners and couple of microtome knives we want to sharpen. We need the fine and coarse abrasive solution that you need to sharpen the knives, does anybody know good source for these solution or an alternative? Vend

[Histonet] Medical Equipment Source

Has anyone had any dealings with a company located in Mars, PA called Medical Equipment Source?? I visited their booth at the NSH and am comtemplating purchasing equipment (new and used) from them.? I was wondering if anyone has used them and, if so, what comments can you share about your exper

[Histonet] anti-Human CD68

Hello, I am looking for a CD68 antibody that will work in Human FFPE tissues, but it needs to be from a species other than mouse (preferably rabbit or goat).  Any recommendations? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA __ Do Yo

Re: [Histonet] free phone consultations

Can The Lab Consultants use their technical expertise to not spam this list? Thanks! Richard Nelson wrote: Hello: The Lab Consultants are experts in lab operations and offer free phone consultations. If you have questions concerning your lab activities please contact us on our toll free l

[Histonet] Job Posting (Boston)

As a growing personalized medicine/cancer diagnostics company, DNAR is seeking to hire: * A full-time Senior Scientist (Ph.D.) with extensive experiences in IHC assay development and commercialization * A full-time Histologist (BS/BA) with HTL (ASCP) Certification and IHC expe

[Histonet] free phone consultations

Hello: The Lab Consultants are experts in lab operations and offer free phone consultations. If you have questions concerning your lab activities please contact us on our toll free line: 866-677-0003. We specialize in lab design, set up, operation and supply and can help you meet CLIA stand

RE: [Histonet] B5 fixative

B5, Schaudinn's, SUSA, Helly's, and Zenker's fixatives contain mercury (II) ions, which are VERY poisonous. Sewage bacteria convert mercury (II) to methyl mercury, which is very, very poisonous. (For a shocking example of mercury toxicity, see the photo essay MINAMATA by W. Eugene Smith.) When

[Histonet] the ubiquitous yet incredibly important timer

We're in the market for new timers. We have been using this kind

RE: [Histonet] Baker's Fluid

Thanks that is the one I am looking for! Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: [EMAIL PROTECTED] -Original Message- From: Weems, Joyce [mailto:[EMAIL PROTECTE

[Histonet] Full Time Histotech Needed

Huntington Hospital in Pasadena, CA has a full time histotech opening for an experienced histotech (2+ years). The position is Monday-Friday, 5:30 am - 2:00 pm, with on-call every 5th Saturday. Experienced applicants may contact me at the number below. Laurie Colbert Huntington Hospital Pas

RE: [Histonet] Baker's Fluid

This was in the archives. Hope it helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax On Wed, 24 May 2000, Barry Rittman wrote: > what you need is "Mollifex

[Histonet] Baker's Fluid

Does anyone have the recepe fpr Baker's fluid? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: [EMAIL PROTECTED] ___ Histonet mailing

[Histonet] Histology Openings in Seattle

Looking for ASCP certified Histotechs for the following openings day position, 7-3:30pm 2nd shift position, 6pm - 2:30am - pays $3 an hour shift differential 3rd shift position, 11pm - 7:30am - pays $10 an hour shift differential Pathologists' Assistant Lead- 10am-6:30pm, M-F IHC Lead

[Histonet] Job Opening

Histology job opening for routine bench work &/ or IHC at Sarsota Pathology/Sarasota, Fl. For more information go to www.sarapath.com . Fax resumes to :(941) 362-8992 or [EMAIL PROTECTED] Thanks, Alice Bonaiuto, IHC Technical Specialist ___

[Histonet] help-fibrotic tissue

I need to finally call on the experts.? I have tried all of the tricks up my sleeve to cut extremely fibrotic Cerivical tissue.? It is not calcified, just fibrotic...it is like cutting glass. Help. Roxanne ___ Histonet mailing list Histonet@lists.uts

Re: [Histonet] getting rid of biotin-avidin background

Why not use an avidin-biotin blocking kit (like those from Vector or Dako for example). They work well, are optiimized already and aren't that expensive. -Original Message- From: Tyrone Genade <[EMAIL PROTECTED]> Date: Mon, 20 Oct 2008 16:07:07 To: Subject: [Histonet] getting rid of b

Re: [Histonet] B5 fixative

For all of the listed B5 substitutes mentioned, how are they disposed of and are they significantly safer? >>> "Piero Nelva" <[EMAIL PROTECTED]> 10/17/2008 17:59 >>> I agree. The Occ Health and SAfety representative is your best bet to consign the B5 to history. Piero Nelva Anatomical patholo

[Histonet] getting rid of biotin-avidin background

Hi all, I have a problem and think I have figured out a solution to it but need some expert advise. I'm using biotinylated GS isolectin to stain frozen brain sections for microglia. The GS isolectin is working very well but the avadin-fluourophore is binding to the endogenous biotin in the tissue

Re: [Histonet] Elastin Aldehyde Fuchsin

The solution does need to be filtered before each use Rena Fail -- Original message from "Adam Boanas" <[EMAIL PROTECTED]>: -- > Hello, > > > > I have been using Gomori`s Aldehyde Fuchsin to stain human skin for > elastin. The stain works fine and is clear and s

[Histonet] Acidified Hematoxylin

Good Morning Everyone, We are in dire straights We are coming to the end of out Acidified Harris Hematoxylin and I was just informed that the company that produces it will not start production until November. We will never make it. Does anyone know where I can get some? Jo-Ann Bader Histo

Re: [Histonet] eosin pH

The eosin should be acidic and you get to that point adding 1% aq. sol. of acetic acid to you prepared solution, at a rate of 1 mL acetic/100 mL of eosin solution. René J. --- On Mon, 10/20/08, Hana Peter <[EMAIL PROTECTED]> wrote: From: Hana Peter <[EMAIL PROTECTED]> Subject: [Histonet] eosin

Fwd: [Histonet] Question on Von Kossa

no - thats the really cool thing - think about it... its that part of the bone which is usually quite hard but softens with stewing/cooking and could be bitten off and chewed (if desired) no demineralisation has taken place yet it is soft enough to slice with a blade and once processed it cuts lik

[Histonet] Elastin Aldehyde Fuchsin

Hello, I have been using Gomori`s Aldehyde Fuchsin to stain human skin for elastin. The stain works fine and is clear and specific. I am using light green as a counterstain. My problem is that once stained, the slides have an excess granular purple "dotted" appearance upon the entire surface of

[Histonet] eosin pH

Hi! Can someone tell me the right pH for eosin y 1% aqueous solution? The one I made from powder has pH 6.3. Is this OK? The commercial one we usually buy is a bit lower (5.7). Thank you in advance! Hana Peter ___ Histonet mailing list Histonet@lists.