[Histonet] Re: Understained my slides with hematoxylin

2008-12-24 Thread Ade Tunde
Hi H&E Stain is very important in the Diagnosis. understaining can be caused by many factors, check the age of your hematoxylin or the concentration of your differentiating solution. Then to restain the stained slide .place the stained slide in 1%  acid alcohol for 2-3minutes and rinse well

Re: Re: [Histonet] Capillary counting

2008-12-24 Thread Rene J Buesa
Use Ulex europaeus lectins. René J. --- On Wed, 12/24/08, TF wrote: From: TF Subject: Re: Re: [Histonet] Capillary counting To: "rjbuesa" , "histonet" , "Nejat Yilmaz" Date: Wednesday, December 24, 2008, 1:48 PM  _filtered #yiv1672193670 { font-family:宋体; } _filtered #yiv1672193670 { fo

Re: Re: [Histonet] Capillary counting

2008-12-24 Thread Andrea Hooper
What animal? What organ? In frozen or paraffin? So any one can provide some cell makers for staining of blood vessels? I only know CD31. 2008-12-25 TF -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.

[Histonet] Michelle Steinkrauss is out of the office.

2008-12-24 Thread michelle . steinkrauss
I will be out of the office starting 12/24/2008 and will not return until 01/05/2009. I will respond to your message when I return. Happy Holidays! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/lis

Re: Re: [Histonet] Capillary counting

2008-12-24 Thread TF
So any one can provide some cell makers for staining of blood vessels? I only know CD31. 2008-12-25 TF 发件人: Rene J Buesa 发送时间: 2008-12-19 04:53:43 收件人: histonet; Nejat Yilmaz 抄送: 主题: Re: [Histonet] Capillary counting Nejat: There are several stains for capillaries: 1- Campbell and

[Histonet] TUNEL on fixed brain tissue

2008-12-24 Thread Guillermo Palchik
Dear Histologists, Does anyone have a good protocol for doing TUNEL on fixed brain slides? Thanks Guillermo Palchik g...@georgetown.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/h

Re: [Histonet] Understained my slides with hematoxylin

2008-12-24 Thread Peter Carroll
> The eosin will tend to leach out in the water rinses and the differentiation solution a touch of acid alcohol doesnt hurt either :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histone

[Histonet] Charcot-Leyden crystals

2008-12-24 Thread alan taylor
Hazel Charcot-Leyden crystals are frequent findings in faecal samples and sputum submitted for microscopical study, the crystals are easily demonstrated,if present, by making a simple 'wet' prep and adding a drop of Gram's Iodine under the coverslip. Charcot-Leyden crystals in faecal submissi