[Histonet] ASCP Recert

2009-01-26 Thread JoeNocito
I know sometimes I am not the brightest bulb in the package, the sharpest knife in the drawer, the sharpest tool in the shed, etc. This is the first time that I'm renewing my certifications with ASCP and do not quit understand the process. I have my PA, HT and QIHC. According to the instructions,

[Histonet] Re: staining for manganese

2009-01-26 Thread Benoît Delatour
Hi, There is a Timm stain - like protocol from Angenstein (Manganese-enhanced MRI reveals structural and functional changes in the cortex of Bassoon mutant mice. Angenstein F, Niessen HG, Goldschmidt J, Lison H, Altrock WD, Gundelfinger ED, Scheich H. Cereb Cortex. 2007 Jan;17(1):28-36) usin

Re: [Histonet] Sectioning on different microtomes

2009-01-26 Thread Victor Tobias
I don't know how these aligning tools work, but I always used an inexpensive level from the hardware store. You need one that can read in the vertical position. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacif

RE: [Histonet] Sectioning on different microtomes

2009-01-26 Thread Jodie Robertson
There are microtome aligners that can help. They are available for Leica, Microm and Shandon Finesse. We used to use them here before we got all of the same microtomes. They worked pretty well. We purchased ours from Newcomer Supply. They're super easy to use and take lots less time than th

Re: [Histonet] staining for manganese

2009-01-26 Thread Rene J Buesa
Gerard: Many minerals can be "seen" in tissues as an unspecific "black" particles when reacting with reagents like ammonium sulfide in the Timm's procedure for copper in Wilson's disease cases. This method requires treating the sections with 0.1N HCl after the reaction to eliminate iron and zinc

Re: [Histonet] ISH probes

2009-01-26 Thread Susan Bachus
There's a wonderful company called Oligo's, Etc. (Wilsonville, OR) that synthesizes very affordable oligoprobes. But you have to supply the sequence information. Susan - Original Message - From: "Sharon Campbell" To: Sent: Monday, January 26, 2009 9:20 AM Subject: [Histonet] ISH pr

[Histonet] New Mexico Society for Histology

2009-01-26 Thread Breeden, Sara
The Board of Directors of the NMSH met on Saturday to discuss the future of the Society. Because membership has declined to less than 10% of eligible members despite concerted efforts over the past five years to increase interest and participation, the decision was made to inactivate the Society.

[Histonet] ISH probes

2009-01-26 Thread Sharon Campbell
Hello Histonetters! We are finding ourselves coming short on the time remaining that we can offer ISH Probes through Ventana. We have not heard when or if ISH (In-situ hybridization) will be offered any more through them. Does anyone out there know of other companies that would offer ISH? Thank y

RE: [Histonet] Re: CPT coding during accession

2009-01-26 Thread Mike Pence
Well said Bob! Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Saturday, January 24, 2009 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: CPT coding during

RE: [Histonet] Sectioning on different microtomes

2009-01-26 Thread Bernice Frederick
I'm on the same page as you Jenaine- we get blocks from all over so we are constantly rearranging the block holder. We also do have a histocollimeter to get the Microms back in line (it doesn't fit on the Leicas. Berncie Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facilit

[Histonet] LFB on GMA sections

2009-01-26 Thread Wilson, Carol
Hi all, Can anyone tell me if LFB stain works well for staining nerves in GMA sections? My pathologist is looking at sections of these stained elsewhere and she does not feel the myelin is staining as it should. Any insight on this particular combination? Thanks in advance. Carol Carol Wils

[Histonet] Microtome alignment

2009-01-26 Thread Webb, Dorothy L
We use the microtome alignment instrument, which can be purchased from several companies. It is in our weekly checklist that all microtomes have to be adjusted (checked) with the microtome aligner. All microtomes are cut at the same angle also! Dorothy Webb, HT Regions Histology Technical Superv

[Histonet] staining for manganese

2009-01-26 Thread Gerard Spoelstra
Hi everybody, I have some fish specimens which have been exposed to toxic levels of manganese. The researcher is hoping to see manganese localised on the gill. Lillie mentions the use of benzidine to stain for manganese. I will try DAB, but my searching on the net has drawn a blank. Gerard Sp