Three levels on all blocks
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne
Clark
Sent: Monday, February 09, 2009 12:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Micr
3 levels.
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or
We cut 3 levels on each block.
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne
Clark
Sent: Monday, February 09, 2009 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtomy of
Hi,
How many of you have a protocol to cut multiple deepers/levels on LEEP
specimens and if so, how many do you cut?
Thanks
Joanne Clark, HT, MLT
Pathology Consultants of New Mexico
Roswell, NM
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Wondering if there are any tech here from Rockford Health System, Rockford, Ill.
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Can any ThermoWave users that microwave prostates please email me offline, if
you are willing to share SOPs
Thanks,
Roxanne
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Hello again fellow histonetters, I have an old artisan special stain/ihc
stain stainer that I'm looking to replace with a newer model, but before
I commit to it I was wondering if anyone else uses any other special
stain instrument that I can look into and compare Thanks for your
help
A
The hematoxylin-phloxin- saffron stain, which supposedly originated at
the Montreal Neurological Institute, migrated from there to
Columbia-Presbyterian Hospital in New York City, where it was used as
the general oversight stain in surgical pathology at least into the
1960's. When I saw it in use t
Hello Everyone,
I have refurbished an old AO 860 sliding microtome. I¹m pretty proud of
myself, I¹ve unlocked the totally gummed mechanism and have put it together
myself. It seems to work very well. The only problem I have is tuning the
sliding block. I have no idea how to approach it, and after
In the UK (where I used to run a clinical lab) we also would turn off
the cryostat in the morning, wait for it to defrost then just before
going home for the evening heat up some formalin in the microwave and
place that in the chamber and leave overnight.
It certainly kills everything, but
Hi Sharon,
HPS is our routine stain (instead of H&E, which 98% of labs use). Not sure
what part of the stain you are having trouble with, but most unpredictability
usually comes in the phloxine/saffron balance.
Some things to watch for are:
-keeping your saffron covered, and I would sugge
Does anyone have any experience with staining for Elastase in
Neutrophils in tissue sections? Any help would be appreciated!
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Thanks very much.
I will extract the lipids first.
2009-02-10
TF
发件人： Geoff McAuliffe
发送时间： 2009-02-10 00:17:29
收件人： tifei
抄送： histonet@lists.utsouthwestern.edu
主题： Re: [Histonet] feulgen stain on brain sections
Dear TF:
Schiff reagent reacts with the lipids (the aldehydes in the
Hi Ann, Are you using the same microscope in the new location? Tom Truscott
USDA-ARS
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
thisis...@aol.com
Sent: Friday, February 06, 2009 1:09 PM
To: histonet@
Dear TF:
Schiff reagent reacts with the lipids (the aldehydes in the lipids) in
the frozen sections so the whole section is pink. You could try
extracting the "offending" lipids with alcohols and xylene, then
rehydrate to water and run the reaction again. A better plan is to
search the litera
Paula:
Any and every step in the histology workflow can and should have a performance
improvement parameter. You refer to IHC repeats as a parameter and in this
case, as in any other, the fact of repeating an IHC by itself is not a good
parameter unless it is associated with the event that deter
Hi Sharon,
During the sixties and seventies the HPS stain was our routine diagnostic
stain. We never had problems with the saffron, but we did take care keeping it
free from all moisture, including the alcohols prior to and right after the
staining with saffron. Method below.
HPS – hematoxyli
Hi,
I would like to know if there are many labs out there doing the
"Haematoxylin, Phloxine, Saffron stain, routinely & how they like it.
We have been doing it & it is lovely when it works but can be very
unpredictable. I am aware of its hydrophobic properties & do what I can
to accommodate this tr
Dear Subscribe,
The New York State Histotechnological Society is pleased to announce
its 2009 annual symposium to be held at the Holiday Inn in Fishkill, New York
on April 24th and 25th, 2009.
Our academic program offers over 25 contact hours of CEU's on a wide range of
topics.
For a copy of
Dear Histonet,
The New York State Histotechnological Society is pleased to announce
its 2009 annual symposium to be held at the Holiday Inn in Fishkill, New York
on April 24th and 25th, 2009.
Our academic program offers over 25 contact hours of CEU's on a wide range of
topics.
For a copy of t
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