Re: [Histonet] semen analysis

2009-02-14 Thread Anne van Binsbergen
thanks for the email we are a hospital based diagnostic lab and currently do all the testing manually i am actually asking what EQUIPMENT is being used for AUTOMATED analysis (count, conc, motility etc) regards Anne 2009/2/15 Malcolm McCallum > semen analysis can be done using various methods

Re: [Histonet] fume exposure

2009-02-14 Thread Emily Sours
Is it so old-fashioned to use xylene in a fume hood? We can't even use paraformaldhyde outside of the hood--Environmental Health and Safety makes sure of that. But we do research, maybe it's different in other types of labs. Emily -- It's like hearing Billy Joel play "Piano Man"-- joyless for all

Re: [Histonet] Problem with Schiff's reagent

2009-02-14 Thread Emily Sours
First, I would try someone else's HCl, especially if it's from a different lab. You never know what happens in other people's labs. Second, this is going to sound really dumb, but you should just remake it. I had the color stay in after charcoal and I finally got sick of filtering it, so I remade

Re: [Histonet] Problem with Schiff's reagent

2009-02-14 Thread Rene J Buesa
Paul: If I remember correctly the last time I prepared my own Schiff reagent was in 1955 and it came beautifully BUT it is a real pain to prepare it, and not really worth the work. Since then I purchase my Schiff and avoided all the trouble. If you have the necessary budget I would advise you to

[Histonet] AUTO: Jacquelyn Grewe/Staff/OhioHealth is out of the office .

2009-02-14 Thread JGREWE
I will be out of the office starting 02/11/2009 and will not return until 02/16/2009. I will respond to your message when I return. Note: This is an automated response to your message "Histonet Digest, Vol 63, Issue 24" sent on 2/14/2009 12:57:22 PM. You will receive a notification for each

RE: [Histonet] Problem with Schiff's reagent

2009-02-14 Thread Thomas Jasper
Hi Paul, Some people may think this is taking the "easy" way out, but...you might consider purchasing some commercially prepared Schiff's. It will (should) be consistent product (consistently produced) and the manufacturer has the responsibility of QC'ing the product before it is shipped and sold

[Histonet] Problem with Schiff's reagent

2009-02-14 Thread pbrunko
Good Morning! I'm a newcomer to the list, and I'll start by pointing out that I'm not a histologist by any means. I am a freshwater ecologist, and we're trying to study mucus secretion behavior in freshwater snails. Last Spring and last summer, we developed a process whereby we could visualize

Re: [Histonet] freezing paraffin blocks

2009-02-14 Thread Rene J Buesa
Not at all! René J. --- On Fri, 2/13/09, Jennifer Anderson wrote: From: Jennifer Anderson Subject: [Histonet] freezing paraffin blocks To: Histonet@lists.utsouthwestern.edu Date: Friday, February 13, 2009, 5:43 PM Hi All. I've just started sectioning Bouin's fixed paraffin embedded human/m

Re: [Histonet] need controls

2009-02-14 Thread Rene J Buesa
The same appendix that you can use as an H&E+ control, usually contains Gram +and- bacteria. René J. --- On Fri, 2/13/09, Jaime Plata wrote: From: Jaime Plata Subject: Re: [Histonet] need controls To: Histonet@lists.utsouthwestern.edu, "Steven Coakley" Date: Friday, February 13, 2009, 5:26 PM

Re: [Histonet] hand processing schedule late mouse embryos

2009-02-14 Thread Rene J Buesa
Under separate cover I am sending a protocol that will work well with your subjects. Since you are doing manual processing you can easily use the reagents I recommend and eliminate xylene and its terpene and alkane based substitutes. René J. --- On Fri, 2/13/09, Nicole Collette wrote: From: Ni

RE: [Histonet] fume exposure

2009-02-14 Thread Smith, Allen
The only staining solutions whose fumes bother me are ammonium sulfide and sulfurous acid. I put the staining jars of these in the fume hood and carry the staining jar with the slides to the fume hood. I bring the water rinse jar to the fume hood to take the slides out. While sulfurous acid i

[Histonet] fume exposure

2009-02-14 Thread zodiac29
To All, I work in a lab where I stain and coverslip everything by hand. I coverslip under a fume hood so there isn't much of an issue there. The main issue is my exposue to fumes while staining. I do not stain under a fume hood. My exposure times to these c hemicals is increasing becuase