[Histonet] User manual for Shandon HistoCenter 2

If you have one (or a xerox copy) to spare I would be very, very, very obliged. Thanks in advance! Yvan. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CPT Coding question 88342 PIN 4 cocktail

We started staining prostate needle bxs w/ PIN 4 (triple stain). 1. My understanding is that we can bill for 88342 x 3 (per specimen) if a comment is made on the results of the nuclear staining and cytoplasmic staining of the DAB and the staining of the Vulcan red. The key is documentation in th

[Histonet] Cutting angle?

Hello Listers, I have inherited a Reichert-Jung 2030 microtome. The knife holder is a bit funky and hard to set the clearance angle. What angle do y'all use? I'm trying to get 4 or 5 degrees. Thanks, Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation

Re: [Histonet] validation documentation for processors

As all validations, you will have to process at least 25 pieces (CAP requires from 25 to 100) of tissue using your old and your new processor simultaneously. Make slides and later prepare H&E and some HC and  IHC procedures using both sets of slides and give them to as many pathologists as you c

[Histonet] validation documentation for processors

A question came up in our lab about validation documentation for our new processor. Does anyone have any creative feedback on how your lab documents validation of machines like processors and stainers? See below> >>>Joseph Fear 03/01/09>>> I'll post the question at histonet and see what i

[Histonet] Sirius red - staining specificity

Hello: Recently, I have used Sirius red (using the Kiernan Method found in Histological and Histochemical Methods, 4th Ed. Scion Publishing Ltd. 2008) to localize collagen fibers in livers of mice exposed to carbon tetrachloride over 5 weeks. Unfortunately, I do not have the capability to exam

[Histonet] Need Histologists ASAP

New private AP lab in Phoenix, AZ., Top pay, great benefits, great hours, it's a new GI lab with state of the art equipment, please do not reply to this email, To submit a resume please apply to twincr...@bex.net _ Hotm

Re: [Histonet] histological stain for pancreatic beta cells

I just searched for this and found in the Histonet archives - currently Google's #1 hit: http://www.histosearch.com/histonet/Sep07/RE.HistonetAzan-MalloryB.html --On Tuesday, March 10, 2009 1:06 PM -0700 Rene J Buesa wrote: Mallory Azan. René J. --- On Tue, 3/10/09, Michele Wich wrote:

Re: [Histonet] histological stain for pancreatic beta cells

Mallory Azan. René J. --- On Tue, 3/10/09, Michele Wich wrote: From: Michele Wich Subject: [Histonet] histological stain for pancreatic beta cells To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 2:34 PM Can I please get some suggestions for histological stains that demonst

Re: [Histonet] CAP

This aspect has to be part of your contingency plan and each lab, with its own idiosyncrasies, has to prepare one, like "what to do during/after a hurricane". René J. --- On Tue, 3/10/09, Behnaz Sohrab wrote: From: Behnaz Sohrab Subject: [Histonet] CAP To: histonet@lists.utsouthwestern.edu Dat

[Histonet] 2009 Tri-State Symposium

You are invited to join the circus and clown around with the histology societies of Wisconsin, Minnesota and Iowa as they host "Big Top Histology" the 2009 Tri-State Symposium, April 29-May 1, at The Madison Concourse Hotel, Madison, Wisconsin. The inclusive registration fee of $100 (+ state membe

Re: [Histonet] histological stain for pancreatic beta cells

We have done an anti-insulin antibody (Abcam) with a hemotoxylin counterstain. Use diaminobenzidine-H2O2  (BioGenex) to visualize the antibody.  --- On Tue, 3/10/09, Michele Wich wrote: From: Michele Wich Subject: [Histonet] histological stain for pancreatic beta cells To: histonet@lists.utsou

[Histonet] Ci-Trol

Hello Everyone, Is anyone familiar with Ci-trol (citrated plasma) for making cell blocks? Someone had recommended a procedure using this, and I wanted to get some feedback on it. Thanks in advance! Jenny ___ Histonet mailing list Histone

[Histonet] histological stain for pancreatic beta cells

Can I please get some suggestions for histological stains that demonstrate pancreatic beta cells? I need something that would be highly specific without using an IHC method. Any advice is greatly appreciated! This communication is intended solely for the use of the addressee and may contain inf

RE: [Histonet] metal molds vs. disposable molds

...which might be the better thing to do then...since it'll all be floating on the surface anyway and we don't want to risk clogging drain pipes with the wax once it cools... --On Tuesday, March 10, 2009 12:59 PM -0400 "Weems, Joyce" wrote: And if you let the water cool, the paraffin will

AW: [Histonet] metal molds vs. disposable molds

We clean our metal molds in an ultra-sound cleaner. Fill it with warm water and a bit of dishcleaner, let it for 10 min. The paraffin easy goes off and swims on the surface. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utso

RE: [Histonet] metal molds vs. disposable molds

And if you let the water cool, the paraffin will harden and you discard it without pouring down the drain... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Tuesday, March 10, 2009 12:22

[Histonet] CAP

Does any one have a procedure regarding : " Handling of work load during instrument failure" .? This would include, VIP,embedding ,etc... Behnaz Sohrab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/

RE: [Histonet] metal molds vs. disposable molds

My issue with the plastic disposable molds is that when you need to re-embed and melt the block down, the mold will curl and lose its shape just enough to impede cutting the second time around. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -Or

Re: [Histonet] metal molds vs. disposable molds

I like my metal molds. They are neat, you get even heat transfer, and are easy to clean when they need it. I clean them by tossing them in some boiling water with an excess of cleaning powder (Alconox or Sparkleen) or any detergent you have on hand (make sure it doesn't get too bubbly and froth

Re: [Histonet] metal molds vs. disposable molds

Hi Sharon-   I've been on your side of this quandry before.  A couple of considerations: what kinds of tissue are you embedding? How many blocks/shift? What is the most important aspect(s) of the following for your situation?   The pros and cons: Metal - pro cool fast easy to remove once cold the

RE: [Histonet] metal molds vs. disposable molds

Metal molds herewe tried the plastic molds but the base would rise up creating an indentation in the block, we needed a flat surface. We wash our molds in an old processor, using the old baskets and running a xylene to alcohol to 95% EtOH run. Then we spread them on a towel to dry and spra

RE: [Histonet] metal molds vs. disposable molds

I agree. I also find you get a more level plane which makes trimming easier. We use both in our group and the individuals that use the disposable tend to use them multiple times before tossing them. I have not had to clean the metal ones I use in years but have heard you can toss them in the

Re: [Histonet] metal molds vs. disposable molds

Metals molds are easier to work with and almost indestructible, but if you have money to throw to the air and patient and time in large supply, use plastic. René J. --- On Tue, 3/10/09, Sharon Campbell wrote: From: Sharon Campbell Subject: [Histonet] metal molds vs. disposable molds To: histon

AW: [Histonet] Tissue RE-processing

You can go back into warm xylen for one hour, that removes the paraffin. Then let the tissue in 2 changes of ethanol abs. , one hour each. That should be long enough for dehydration and hardening the tissue. Then go into xylen again for one hour, and then let it sit in paraffin for 2-3 hours. The

[Histonet] metal molds vs. disposable molds

Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Di

[Histonet] (no subject)

Does anyone have an opinion on Distilled vs DI water?  Is there a difference in quality? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Looking for Darcy Peat

I worked with Darcy in Hawaii years ago, and I think someone said she may have moved to Washington State. Darcy, are you out there? Does anyone know where she is? Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utso

[Histonet] RE: Cassettes and Processing and Fixation ~ Oh My!

Laura, 1. In the past we used different colored cassettes for rush, non rush and special projects. Then we noticed that we still treated every case like it was retained/rush!! So we dropped back to one color for all our regular cases; microscreen cassettes for anything that might slip through and

[Histonet] 2 histology positions at UCLA

Hi all, We still have 2 positions available here at UCLA. The first is a histotech I position, a 50/50 clinical/research position working with us in the clinical lab as well as in the lab of one of our urologic pathology faculty (a great guy I might add). While not a requirement, if you have

[Histonet] Managment Opportunity In Histology-in Mass

Good Morning, My name is Alyssa Peterson, and I am the Director of Lab/Pathology recruitment, and I am contacting you about a permanent, full time, Monday-Friday Day Shift Histology Management position located in about 20 miles North West of Boston (You would not have to travel into Boston)! If y

[Histonet] Tissue RE-processing

Hi to everybody, I have a problem with a recent processing tissue. Normally I process tissue that is very small such as 3mm thick of vessel rings or small cubes of myocardium. Couple of days ago we process a batch of myocardial sections that are really thick. I thought might be thick enough my pro