Hi all,
I am researching the Leica Bond Max immunostainer and I like its features as
advertised.
The advertisement however states that the system is completely open, but after
discussing it in length with the Leica rep I am finding that this is actually
not so...
We are a research core and
Hey, we can't help it if your parents were big fans of his. He is a great song
writer.
;-)
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
--- On Tue
Tony,
I have been lead to believe that after von Kossa staining, Silver
carbonate dissolves in sodium thiosulphate and cannot be demonstrated
with von Kossa's technique. Though I have not confirmed this
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior
We also make our own TBS for our Dako autostainer, same as Jan. I have used
PBS instead of TBS at times, when transfering a bench run where I used PBS /
tween (my standard buffer) to the machine. I wonder if TBS really does give
better results than TBS, when averaged out over all Abs? I do kn
I have heard not to use freezing spray on blocks/sections that are
destined for IHC but I never heard the reasoning behind this warning.
I've always steered clear of it when cutting for IHC and instead will
bury my block in the frost of our freezer until its good and cold.
Works fine.
Linda A. Seb
Bill:
I think you refer to the National Hansen's Disease Center at the Louisiana
State University in Baton Rouge. If you contact the LSU for sure they can tell
you about it.
René J.
--- On Tue, 3/31/09, WILLIAM DESALVO wrote:
From: WILLIAM DESALVO
Subject: [Histonet] Looking for Lab
To: "hist
Hi - I think it is the Pennington Labs in BR. If you can contact Cheryl Crowder
in BR at ccrow...@vetmed.lsu.edu
she will probably know for sure.
Judy in Washington (A tiger grad- geaux tigers!)
On Tue, 31 Mar 2009, WILLIAM DESALVO wrote:
I have heard that there is a research lab in Baton Rou
I have heard that there is a research lab in Baton Rouge, LA that specialises
in Leprosy cases. I have a request from one of my Derm Paths to locate so that
we can send a difficult case. I would apprciate any information. Thanks
William DeSalvo, B.S. HTL(ASCP)
Production Manager
Sonora Que
Hi Histonetters,
I have someone here who is having problems with their samples (mouse lung
and mammary). It appears that the sample is very brittle when she cuts and
in addition, some of the paraffin around the sample in the cassette is very
white in color instead of the normal translucent color.
We file all our blank slides with the stained slides. That way we can always go
back to them rather than cutting any more on an already minute piece of tissue
remaining. Also, for legal reasons, what if the blanks that are discarded
contained tumor? (or NO tumor) IE: Showing something other t
Here at Hartford Hospital we do the following for biopsies:
Prostate: 5 slides (1, 3, and 5 - H&E/2 and 4 - unstain)
Lung: 5 slides (1, 3, and 5 - H&E/2 and 4 - unstain)
Breast: 7 slides (1, 4, and 7 - H&E/ 2 ,3, 5, and 6 - unstain)
Transplant heart: 8 slides (1, 3, 5, and 7 - H&E/2, 4, 6,
Hi,
Does anyone know of a place that I can get histology training? I am
specifically looking for a place in the US or Canada to go to for a couple of
weeks. I am not able to take courses that are 1 to 2 years long. Thanks in
advance!!
Kimberly Poole B.Sc
Casualty Management Section | Se
With the lever loose, tight the two screws in a way that the blade is free and
can be moved in any direction. The lever will lock the blade and it should be
tighten "all the way".
René J.
--- On Tue, 3/31/09, David Waugh wrote:
From: David Waugh
Subject: [Histonet] AO Blade holder
To: histone
In our lab many of the histotechs use Cytocool when cutting paraffin blocks.
Has anyone heard of this having a negative effect on IHC?
Leslie McCormack
Baptist Medical Center
Jacksonville, FL 32207
_
Express your personalit
Hi David: When I used an AO with a disposable blade holder. I had to test a
blank paraffin block to get the blade the right tightness. There was no set
tension that I can remember. What I would do it tighten the blade holder until
the disposable blade was held securely. Test the knife with
I have a question regarding a AO disposable blade holder (Cat# 824).
This link should show a picture of a similar one www.usedmicroscopes.com/images/AO820Microtome.jpg
The blade is tightened with a lever on the right that rotates a d-
shaped cam. Two screws are used the set the tension that
Hello,
Does anyone have only procedures online? All of ours are online, but we
still keep a paper copy of each in a manual in the lab. I would like to
get rid of the manual, but then how do you record pathologist sign-off
on all new or revised procedures?
I'm curious to hear what other labs a
I have a question regarding a AO disposable blade holder (Cat# 824).
This link should show a picture of a similar one
www.usedmicroscopes.com/images/AO820Microtome.jpg
The blade is tightened with a lever on the right that rotates a d-
shaped cam. Two screws are used the set the tension that
I have a question regarding a AO disposable blade holder (Cat# 824).
This link should show a picture of a similar one (http://
www.usedmicroscopes.com/images/AO820Microtome.jpg)
The blade is tightened with a lever on the right that rotates a d-
shaped cam. Two screws are used the set the tens
Hi Debi -
I've seen protocols similar to what you describe, and in my experience, they
are almost always a result of someone, at some point in time, cutting through
(wasting) a critical small specimen after the initial H&Es were cut or during
multiple levels. Perhaps the case can be made that y
I have a question regarding a AO disposable blade holder (Cat# 824).
This link should show a picture of a similar one (http://
www.usedmicroscopes.com/images/AO820Microtome.jpg)
The blade is tightened with a lever on the right that rotates a d-
shaped cam. Two screws are used the set the tens
We have had great success with our Sakura Xpress.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickro
Yeah, hilarious. I swear, I haven't heart THAT one before
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)"
To: "Edwards, R.E." ; histonet@lists.utsouthwestern.edu
Sent: Tuesday, March 31, 2009 10:18:36 AM
Subject: RE: [Histonet] onalighternote
I wanted to ask how
Milestone makes excellent microwave processors for small GI biopsies and for
large tissues.
Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical Lab.
301-665-4980
fax 301-665-4941
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@list
We are thinking about Microwave Tissue processors. Unfortunately I have been
given very little time to present a budget request. Can anyone comment on a
recent purchase of a microwave tissue processor and their experiences?
Thanks
Jim Vickroy BS, HT(ASCP)
Technical Supervisor - Surgical an
Yes please!!! I am desperate for a new microtome!!!
Caroline
Caroline Miller M.Sc DIC AIBMS
Co-manager
Gladstone Microscopy and Histology Core
J David Gladstone institute
1650 Owens Street
San Francisco
CA
94158
www.gladstone.ucsf.edu
On Mar 31, 2009, at 6:29, "Cathy Mayton" wrote:
I have a
We do not throw them out, we file them with the stained slides for future
reference.
Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-b
I agree with Lorraine on this one.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildrens.org
-Original Message
Hi, I am interested to look at the fibrin, muscle, collagen and e
lastic fiber distribution in the artery in the plastic resin section.
Any s= uggestion for the specific staining that is required? I know
that Movat pen= tachrome will stain muscle, collagen and elastic
Thank you for your very prompt response. I do agree that we are here for
the patient.
Unfotunately I am under the gun to bring down costs and I thought that
might be one way. Thanks again, and look forward to other responses.
Debi
From: Lorraine Cornett [
We are currently handling all prostate, lung, heart, and breast biopsies
as fine needle biopsies (FNB). We cut 10 slides 3 of which are used for
H&E's and the rest as unstained plus slides. What has happened is that
we throw thousands of unused expensive slides a year because they are
not used. Is
I think you meant to say CD. :)
Have a grand day!
Bill
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards,
R.E.
Sent: Tuesday, March 31, 2009 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [H
I wanted to ask how Elton was but thought that was a bit too easy...
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.e
I have just had s from Bernie Taupin and Paul Schofield, is this a
record??.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez
Sent: 31 March 2009 15:08
To: Bernie Taupin; histonet@
I think Ford gets the message...GET ON WITH IT! Antibody talk anyone?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin
Sent: Tuesday, March 31, 2009 6:50 AM
To: histonet@lists.utsouthwestern.e
> I can personally attest that Ford must have been having a VERY bad day indeed.
Although I can empathize that this might be the case, I do find it curious that
we've heard nothing from Mr. Royer since his little outburst.
I, for one, wouldn't mind an apology to the list for your antisocial beha
I have an AO 820 microtome with gray cover free to a good. It still works
great and was replaced with a new microtome we used in the lab. Has been
sitting idle with a dust cover for many years. Just pay the shipping to its
new home.
Cathy A. Mayton
Wasatch Histo Consultants, Inc.
80 Youngb
Any lab performing PDGFR (Platelet Derived Growth Factor Receptors)?
Please contact me directly.
jerry.santi...@jax.ufl.edu
Jerry Santiago
Shands Jacksonville
Jacksonville, Florida
904-244-6149
___
Histonet mailing list
Histonet@lists.uts
Michelle:
The motorized microtome was introduced specifically to alleviate the
carpal-tunnel syndrome quite frequent in histotechs that have been sectioning
for years.
It is very advantageous and can take care of the trimming necessary to reach
the area that is going to be sectioned and even the
Hi
I would like your opinion of the manual vs semi/fully automated rotary
microtome, especially with regard to stress on the hands.
Thanks
Michelle
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic Medicine
Faculty Health Sciences
University of Cape To
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