I think our problem may very well be the slides. We tried many slides in the
past and always came back to the superfrost brand that was most reliable, but I
have found different opinions on these slides ;ately. I think it is time to
change brands. Thank you all...Hope to see everyone in Birmingh
I second that. Marsha is the loveliest person I know.
- Original Message -
From: "Denise Piontek"
To:
Sent: Wednesday, April 08, 2009 8:49 PM
Subject: RE: [Histonet] Controls needed!
Newcomer Supply has been a reliable control source, when necessary the have
"customized orders".
I just had the same problem with a bad lot of charged slides. It is, indeed,
maddening.
>>> "Joe Nocito" 4/8/2009 5:08 PM >>>
another issue could be the mixer blowing too hard. I've had that happen too.
JTT
- Original Message -
From: "Kari Bradshaw"
To: "Dana Spears" ; ;
"Phyllis Tha
Hi All,
I was deleting my unwanted e-mails last night and came across this link which
is amazing and safe. If you haven't previewed this, you may want too. There
is a higher power watching over us!
http://www.dailymail.co.uk/legacygallery/gallery-9139/Hubble--The-amazing-space-photographs-uni
Are there any used equipment companies out there that would be
interested in two used "Upper part for disposable blade carrier "E" '
for a Microm HM325 microtome?
Laurie Colbert
Huntington Hospital
Pasadena, CA
(626) 397-8620
___
Histonet mailing
Don't forget the effects on decalcified Bone Marrow Cores. Even if you use
EDTA decalcification solution, there will be diminished FE staining on Bone
Marrow Cores or any other tissue that is decalcified and then stained for FE.
Regards,Akemi
Akemi Allison-Tacha BS, HT(ASCP)HTL
Histology Manag
Usually not, unless the tissues remain too much time in the decalcifyier,
meaning more time than needed to decalcify. For IHC the adverse effect is
greater and because of that, when possible, the decalficication should be done
with EDTA.
René J.
--- On Thu, 4/9/09, kyle ayres wrote:
From: kyl
Is anyone aware of a reference lab that has INI1 antibody one their
menu?
An inquiring neuropathologist wants to know...
All positive responses will be happily forwarded to him...
Thank you!
Sally Ann Drew, MT (ASCP)
sd...@uwhealth.org
IHC/ISH Lab DB1-223, Mail Code 3224
600 Highland Ave.
Madison
Are there negative effects associated with acid decalcification and special
stains, for example, AFB, GMS, and PAS (for fungus). All tissues are FFPE.
Thanks,
Kyle Ayres HT BS
Nacogdoches Memorial Hospital
___
Histonet mailing list
Histonet@lists
I used the Tdt from DAKO using their autostainer, diluted 1:10, HIER at pH8 and
thymus as control.
René J.
--- On Thu, 4/9/09, Taylor, Jean wrote:
From: Taylor, Jean
Subject: [Histonet] TdT
To: histonet@lists.utsouthwestern.edu
Date: Thursday, April 9, 2009, 2:11 PM
I'm wondering where labs
I am seeing this when I work with mouse tissue. After necropsy I extract the
tissue from the mouse, cassette it, put it in fresh 10% NBF and let the tissue
fix 24hrs before processing. I also see an edge effect when I fix with 4% PFA.
During IHC-P I usually retrieve with a pressure cooker (120C)
Sam,
Could you give us a few facts from your protocol? They may help pin down
what might be causing your edge effect.
How long does your tissue stay on the grossing/trimming table before being
put into fixative?
What type of fixative are you using? How fresh is it?
How long to you fix?
Wha
Hi All,
I am having variable staining when performing IHC. I seem to get an edge effect
where the edge of the tissue shows increased DAB staining compared to the middle
of the tissue. I have tried testing many fixation variables and still haven't
found an edge affect culprit. I'm wondering if a
NCCLS is now known as CLSI. They have a standard (document) for the
writing of procedure manuals. CAP will require this, so you may want to
purchase that document now.
"Putnam, Jodi"
Sent by: histonet-boun...@lists.utsouthwestern.edu
04/09/2009 09:51 AM
To
cc
Subject
[Histonet] New la
I'm wondering where labs are purchasing their TdT antibody from. I'm
currently using the Dako Autostainer and would be interested in any
information on the procedure as well.
Thank you!
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
Hi, I called the NSH and they said I might have better luck posting on the
histonet but they are sending me all that they have in the way of help in
writing manuals for the lab. I am working in a new lab that just opened a few
months ago, all derm, building from the ground up. I have all my log
This person will need to be based on the West Coast, California preferred.
Leica Microsystems is a leading global designer and producer of
innovative high-tech precision optics systems for the analysis of
microstructures.
It is one of the market leaders in each of the fields of Microsco
I have noticed in the past several posts looking for traveling histo techs.
Would one of these agencies please contact me off list?
35+ years of experience recently become available.
Robert Schoonhoven HT/HTL (ASCP)
269-615-0576
___
Histonet
Ann,
I was able to investigate this unit at Milestone Medical as their US
headquarters is located locally here in Kalamazoo, MI. They have an excellent
line of products and I recommend them highly.
Disclaimer: I have no commercial interest in Milestone Medical.
Regards,
Bob Schoonhoven, HT/H
Position Open in Mass
Good morning and Happy Spring to everyone. I have a full time permanent
position open in Burlington, Ma area. I have included the details below, and
don’t forget about our $1000 referral program in place. Please forward this
to whomever you feel fit, and if we place one of
Hi, all
We get some money recently and want to spend it on the HISTOS 5
proceesor from Smilestone. But we are not sure whether it is worth
purchasing. Does anyone here have any experiences or suggestions
of it?
Thank you,
Ann Fu
University of Flodrida
__
Thank you all for taking time to respond to my inquiry about Formula 83. We
received a free sample and will perform some trials and I will post our results
on the Histonet asap. Of course, there can always be hidden challenges
bringing reagents over the Canadian border, so that in itself can p
Dear List:
I strongly suggest that you NOT use any links offered by people with no
name and no affiliation. Yes, there are good spyware detection programs
available at no cost (Adaware for example) but I don't think this is one
of them. There are at least 3 potential saboteurs on this list.
With all due respect René; I have been using Formula 83 with my coverslipper
with no problems at all though have a glass coverslipper not a tape.
Linda
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email
I used to run a negative control per case or per block within any given case
when doing IHC, but not for reagents.
René J.
--- On Wed, 4/8/09, Sheila Haas wrote:
From: Sheila Haas
Subject: [Histonet] Negative IHC controls
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, April 8, 2009, 2:
Jacqueline:
Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers,
is recyclable and while some people have been using it for more than 20 years,
others find it irritant.
René J.
--- On Wed, 4/8/09, Jacqueline Farnsworth
wrote:
From: Jacqueline Farnsworth
Subject: [Hi
Thank you to everyone who responded!! I was given the cell pellets already in
the matrix so I only had to process!! It was easier than the mouse tissues!!
Since we do so few 20-40 at a time I do everything by hand...no automation in
our lab!!
-Original Message-
From: Bernie Taupin [
Fellow histology and related fields professionals,
I'm not opening this. From a call name of "u know
(u_deserve_2_k...@yahoo.com)." The last time I opened something like this,
from a name like this, I ended up owing money for Viagra treatments for Panda
bears or s
Please, stop spamming this list.
Comment if you know something useful. Otherwise keep it.
I don't mind what you had for breakfast in June 1st last year or your
opinion on my clothes' colour.
Believe it or not, there IS a real life ( link:
http://en.wikipedia.org/wiki/Real_life_(reality) ), and
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