Hi All...Again. I guess I have a lot of questions lately. I have asked this
before but I would like to ask it again. What are people doing for QA/QC? I
have been asked by management to write a QA manual. I have some ideas but not
really sure where to start. Thanks in advance for any input.
I am a MLT registry eligible technician. I received my MLT training in the
military. I would like to get into histology. I am looking for an opportunity
to gain experience, either through working or and internship. I have no prior
histology training but I am eager to learn and I am a fast le
Embedding frozen tissue in Pwax is very possible, Tomasz.
If your tissue has been frozen NOT using OCT, just place the frozen tissue into
acetone that has been cooled to -20C.
x3 changes of -20C acetone , then immediately place tissue into molten pwax x3
changes.
Agitate frequently, gently.
I as
thanks to the reply.
some antigens will be damaged by PFA fixation and can not be retreieval.
the shock frozen section with acetone is of quite bad tissue quality. therefore
I am trying alcohol!
2009-05-16
TF
发件人： Geoff McAuliffe
发送时间： 2009-05-15 23:55:15
收件人： tifei
抄送： Histonet@lists
I agree with Paul Monfils, there needs to be fixation prior to embedding in
paraffin plus the tissue needs to be infiltrated or the paraffin will not
penetrate the frozen tissue. Even if you are not looking for infiltration, the
hot wax will eventually harden and the wet tissue will fall out or
> Does anyone know who sells the KP Marker PLUS? I can't seem to find them.
Thanks for any help.
Did you try Googling it?
http://www.google.com/#hl=en&q=KP+Marker+Plus&btnG=Google+Search&aq=f&oq=KP+Marker+Plus&fp=-dx2DTn7Pl4
On Fri, May 15, 2009 at 12:12 PM, Metzger, Kenneth <
kenneth.metz.
By the way, they are only $55/box... :-)
--On Friday, May 15, 2009 1:17 PM -0400 Merced M Leiker
wrote:
Indeed! My box of 12 pens just arrived today. Cat# KLI KPPEN.
www.mercedesmedical.com
Merced
--On Friday, May 15, 2009 11:16 AM -0500 "Molinari, Betsy"
wrote:
Mercedes Medical 800-33
Indeed! My box of 12 pens just arrived today. Cat# KLI KPPEN.
www.mercedesmedical.com
Merced
--On Friday, May 15, 2009 11:16 AM -0500 "Molinari, Betsy"
wrote:
Mercedes Medical 800-331-2716
Betsy Molinari HT (ASCP)
Texas heart Institute
Cardiovascular Pathology
Houston,TX 77090
832-355-652
Ken
I am sorry that our contact info isnt on the pens themselves. We are the
U.S. source for the Klinipath KP Pen.
Thanks
Dave Johnson
Mercedes Medical
7590 Commerce Court
Sarasota, FL 34243
800-331-2716
www.mercedesmedical.com
-Original Message-
From: histonet-boun...@lists.utsouthw
I assume you mean the tissue was frozen without fixation, not that you want to
put it into paraffin without fixation? No tissue will withstand paraffin
processing without fixation. The best way to turn a frozen tissue into a
paraffin embedded tissue is to drop the frozen tissue (either plain or
Mercedes Medical 800-331-2716
Betsy Molinari HT (ASCP)
Texas heart Institute
Cardiovascular Pathology
Houston,TX 77090
832-355-6524
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger,
Kenneth
Sent: Fr
Does anyone know who sells the KP Marker PLUS? I can't seem to find
them. Thanks for any help.
Ken
Kenneth G Metzger HTL(ASCP)
ARUP Labs
Histology Supervisor
500 Chipeta Way
Salt Lake City, Utah 84108-1221
kenneth.metz...@aruplab.com
(801) 583-2787 x 3101
- --
> Good luck freezing a brain that is full of alcohol! Have you checked the
freezing point of alcohol?
> Why are you doing this?
I think that moire often than not, people ask before they think ;-)
On Fri, May 15, 2009 at 11:56 AM, Geoff McAuliffe wrote:
> Greetings TF:
>
> Good luck freezing a
> It seems that people are suing for any and everything they can these days
I would too if I was misdiagnosed or otherwise the victim of malpractice
involving incorrect/erroneous histopathology. Don't disrespect such victims
by lumping them in the "damn litigious people these days!" crowd.
On
Greetings TF:
Good luck freezing a brain that is full of alcohol! Have you checked the
freezing point of alcohol?
Why are you doing this?
Immersing a whole brain in 70% alcohol, why??
30% sucrose is a cryoprotectant so the brain is not full of holes from
ice crystals.Alcohol defeats this purpo
Hi All,
I am just wanting to get an idea of how many of you charge law firms or
legal gathering sites for making copies of pathology reports and sending
slides and blocks. It seems that people are suing for any and everything
they can these days. I would also like to know what people are charging
Hi, i dont want to use PFA for the brain fixation (rat).
Now I tried to perfuse the rat with saline, followed with 70% alcohol. Then I
do post-fixation at room temperature for 24 hours.
I also tried saline perfusion, then I directly put the whole brain into 70%
alcohol.
Is this fine?
Also, for d
Google is always a good first step:
http://www.google.com/#hl=en&q=CD25+in+canine+tissue&btnG=Google+Search&aq=f&oq=CD25+in+canine+tissue&fp=-dx2DTn7Pl4
On Fri, May 15, 2009 at 4:02 AM, NEIL MACINTYRE <
neil.macint...@btinternet.com> wrote:
> Hi everyone
>
> I have a researcher wishing to look at
> is there any good method for embeeding fresh frozen tissue (without any
fixation) in paraffin?
Wouldn't the molten paraffin would melt the frozen tissue? LOL
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why would you want to do this? - the water contained in the fresh tissue
will not "mix" with the paraffin wax. There would be little purpose in
trying.
On 5/15/09, Tomasz Bonda wrote:
>
> Hello,
> is there any good method for embeeding fresh frozen tissue (without any
> fixation) in paraffin?
> I
Hello,
is there any good method for embeeding fresh frozen tissue (without any
fixation) in paraffin?
I would be grateful for ANY suggestions.
T. Bonda
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Hi everyone
I have a researcher wishing to look at CD25 in canine tissue. Has anyone any
information that could help?
Many thanks
Neil MacIntyre
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