If they are cytospins they will not need dewaxing so no vegetable oil.
But having said this there is a school of thought that suggests that the
use of vegetable oil may aid in the identification of Fite positive
organisms (by ?coating the bugs).
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAna
Hello Histonet.
Has anyone had experience and good results doing AFB Fites on cytospin slides
from BAL's?
Do you use the peanut oil/xylene (PO/Z) solution or just 100% peanut oil? Or
does the cytospin slide need any peanut oil?
After the deparaffinization step, if it can be called that, we are go
I am wanting to explore all my options. I am having some "real" issues
with our automated special stainer and I am considering going to a new
system. Can anyone tell me what systems are out on the market that
preforms hands-free special stains. All options are open. Vendors
welcome to reply.
Mik
Hi histonetters,
Please help. What is difference in procedure for IHC between 10%Formalin fixed
tissue and in Bouin's fixed tissue? I used to use 10%FFPE tissue with Citrate
buffer Antigen Retrieval for my IHC. Do I have to change my protocol for the
Bouin's fixed tissue?
Hope for you soon re
hello, will someone with a tried & proven protocol for processing
heartworms for paraffin sectioning please share your protocol with me
ASAP? My histonet archive search was unsuccessful. Thanks! Atoska
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I think your techs have misplaced a digit. It should only be about 5-10%
ammonium chloride. (plenty strong enough for ME thank you.) And no, we don't
have IHC problems later.
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Saundra Johnson
Sen
Hi, Does anyone know how to contact Larry Mahler. Is or was with Scientific
Instrument Service in TN.I am looking for some help with a IL MVP I tissue
processor. I would like to also find anyone who might know about where to
find parts for this older machine.
LeRoy Brown HT(ASC
I tested the sequential IHC protocol...though a bit different.
I am using two mouse monoclonal antibody, and Goat-anti Mouse Alexa 488 , 568,
respectively.
The 2nd secondary Ab will bind to the 1st primary antibody, for sure, with
almost >80% background (you can visualize very dark cell morpholo
Rene,
I think you're correct. In many of the old/antique supply catalogues there are
listings for several types of "spring" coverslip clamping forceps that were
used to "compress" the coverslip while drying the mountant, and to distribute
Canada balsam evenly beneath the cover. When I used to ma
Hi Frauke,
I've read the abcam protocol. They don't indicate what species the ab's are
from. Or I've overlooked it.
What is the reason, why the second secondary ab doesn't bind to the first
primary ab?
Gudrun
-Ursprüngliche Nachricht-
Von: Dr. Frauke Neff [mailto:ne...@staff.uni-marburg.
Check out the Sigma-Aldrich website at: www.sigmaaldrich.com
91A is part of a kit & 90A1 is the "select reagent packaged in gelatin
capsules."
Lynette
Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503
ph: 810-257-9948
fax: 810-762-7082
>>>
What are you trying to track and what do you want to accomplish? Are you going
to track and record defects/errors produced in your process, performance of the
individuals, quality of the processes or all? This is a big project that will
require setting up "controls" to monitor performance and i
I have been a user of the Sakura Xpress 120 (2 units) for 5+ years and find
them to be very reliable w/ excellent performance and they fit into the
continuous workflow process exceptionally well.
William DeSalvo B.S. HTL (ASCP)
Production Manager, Sonora Quest Laboratories
Chair Person, NSH
I also have a number of these blades and an old knife sharpener, which I
would like to get to a good home.
Esther Peters
George Mason University
Wallen, Jeannette B wrote:
Does anyone still use the steel microtome blades that need to be sharpened? We
have found some of these blades in our lab
Looking for the order number for Alpha-naphthyl-butyrate.
I will be using it for the NSE procedure. I spoke with the Fisher 800
number and they were unable
to help me.
We use Fisher or Sigma products.
Thanks
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Never heard of that, but perhaps she is referring to placing some small weight
over the coverslipped section to help eliminate the bubbles by the weight,
other than that, that is totally new for me.
René J.
--- On Mon, 5/18/09, Andrea Grantham wrote:
From: Andrea Grantham
Subject: [Histonet]
I have not compared 60 to XYL but started with XYL from the beginning.
I use n-butyle acetate (http://en.wikipedia.org/wiki/Butyl_acetate) for
clearing, its MSDS is quite satisfying and it smells way better.
Mike Tighe wrote:
Has anyone compared Cytoseal 60 with Cytoseal XYL? I clear with Xyl
Does anyone still use the steel microtome blades that need to be sharpened? We
have found some of these blades in our laboratory and will dispose of them, if
no one has any use for them.
Jeannette B. Wallen, HT (ASCP)
Histology Specialist/Histology Team Lead
Hennepin County Medical Center
Anatom
Good Monday morning!
This may sound crazy...or not. I have to admit that I have never heard
of this device before and I was wondering if any of you out in
histoland could tell me what this is and where one might go to get it,
if it were available for sale.
Here goes:
A student who was hav
Hi TF,
we do IF with two 1.ABs out of mice using a sequential protocol from
abcam: www.abcam.com/technical
We perform a second blocking step with serum (higher conc. than the
first one) after the first Fluorescence was added, do not perform
another retrieval procedure (my ABs need the same)
TF,
Two things you can do;
If you are using a chromogenic system (not fluorescence) you can use DAB as the
first chromogen. The DAB polymerized product will cover the first primary and
prevent cross-reaction.
Or, if using IF you can use unlabled Fab fragments to coat the first primary.
For
We have a plentiful amount (hundreds) of malignat melanoma blocks that we are
willing to trade some for any ER/PR + tissue, colon cancer, or any other
uselful IHC control tissue. Any takers?
This message contains confidential information and is intended only for
Thanks all, Merced M Leiker is right: the double IHC procedure using two
primary antibodies from same species.
Some expert just sent me their procedure of Heat-interval based inactivation
of the primary antibody for first antigen.
I am just seeking for a chemical way at room temperature.
2009
We have an Excelsior that is about 5 years old. We have had a lot of
issues and have had service too many times in the past year to count.
We are looking at replacing it. Perhaps we got a lemon, but I don't
think I could recommend it based on our experience. Are there any other
processors anyone w
I am completely confused.
IF you have an epitope in the tissue and you react with it an specific antibody
"that is it". The epitope will have reacted and I do not see any way in which
you can add another antibody to that initial epitope. From the reactive point
of view, the epitope is "blocked"
Hi everyone.
I have a researcher that would like to look at CD25 in canine tissue. Has
anyone ever used this antibody? Any information would be much appreciated
Neil MacIntyre CSci FIBMS
Laboratory Manager
Veterinary Pathology Unit
Easter Bush Veterinary Centre
Nr Roslin
Midlothian
EH25 9RG
p
Hi Bernice,
I noticed a post in the histonet archives about using ammonia water to soak.
Could I ask your opinion about how ammonia water could effect the IHC
staining? I have come to work here and the two techs here soak in 50% ammonia
water. That seems way to strong to me. What do you t
I was just wondering what everyone's thoughts were on thermo products.
I have been demo-ing an Exlcelsior Processor and was wondering if
anyone had any problems.
Let me know.
LeAnn Murphy
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That is Mercedes Medicals LIST Price for those accounts that order
online and order 1 box per year. If you order through a Histology Sales
Rep (800-331-2716) You may enjoy a discounted price. Because Mercedes
Medical Reps are nice and appreciate great customers!
-Original Message-
Fro
Hi everyone.
I have a researcher that would like to look at CD25 in canine tissue. Has
anyone ever used this antibody? Any information would be much appreciated
Neil MacIntyre CSci FIBMS
Laboratory Manager
Veterinary Pathology Unit
Easter Bush Veterinary Centre
Nr Roslin
Midlothian
EH25 9RG
pho
Hello Jennifer,
We are a hospital site and operate 2 Dako Autostainers. We collect our DAB
waste into empty plastic carboys from Coulter Diluent used in our Hematology
Department. These containers are encased within a cardboard cover. They are
very convenient as we can place the hose directly in
Hi TF and all interested,
I think I know what you want, but unfortunately I don't know how to answer
your question (it is something I'd like answered myself!!) To re-word for
the sake of all interested:
You want to perform double-immunofluorescent staining using 2 primaries
that were raised
Has anyone compared Cytoseal 60 with Cytoseal XYL? I clear with Xylene and use
cytoseal 60 (Toluene)to coverslip. Seems like it would make sense to use
Cytoseal XYL (Xylene) but they only sell in a case and I can't get a sample. I
would be interested to know if anyone has a preference.
Thanks!!
I am working in immuno Lab doing hgh volume and i need resource where i can
have some continuing education.Thanks if any one can help.
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