Robert
If you are using formalin fixed paraffin embedded human material, Sigma have
a very nice mouse monoclonal that works beautifully. The cat no. is M-4403
(clone HM-2).
Cheers
Jim
Jim Manavis
Laboratory Manager
Hanson Institute
Centre for Neurological Diseases
IMVS, Adelaide, SA, 5000
Austr
To all histonetters,
I am currently working with the MMA embedded section. I need to attach
about 100micro thick sample to glass slide prior to staining. The sample
is direct from saw microtome. Secure attached is needed so that the
section will not detach from glass slide while staining.
I h
Yep,
You can use alkaline phosphatase labelling instead of peroxidase
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westme
Richard,
Cherukian's Phosphotungstic Acid Haematoxylin works well
Principle:
Cherukian's modification employs an eosin solution that stains the erythrocytes
red and differentiates them from the blue fibrin.
Fixation: 10% buffered formalin.
Microtomy: paraffin sections at 5μm.
Co
