RE: [Histonet] Storage of tissues in PFA before dehydration andembedding

Nick, Where did you hear that "storage in PFA for longer than 24 hrs may adversely affect my tissues"? The beauty of 4% formaldehyde (especially if it is buffered) is that it does not adversely affect tissues, in fact it protects them brilliantly from the rigours of subsequent processing. If it is

RE: [Histonet] TSH receptor

Thanks for the suggestion Bernice. I've been in contact with abcam (I maybe should have mentioned this). They have several TSH Receptor antibodies but unfortunately none are validated for IHC in sheep. They suggested a couple of possibilities based on cross-reactivity in pigs but obviously can

[Histonet] recommendations for anti-rat c-kit?

Can anyone recommend a reliable anti-rat c-kit (CD117) antibody that they have had good experience with on frozen sections? Thank you! Merced M Leiker Research Technician II Cardiovascular Medicine 361 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo,

[Histonet] Histology Positions-GA & PA

Hello, We are seeking a Histotechnologist candidates for our client in *Evansburg**, **PA** (30 minutes northwest of **Philadelphia**) and in Augusta, GA.* This is an opportunity to work for a leading/well known company who plans on a 250% growth rate in the coming year. Our client has a state

RE: [Histonet] microtome calibration

Diane: I provide microtome service calibration etc. Please email me if I can be of service. References upon request. p...@firnschild.com Paul Paul M. Firnschild QA Support Services, Inc. 404.291.3715 -Original Message- From: Dianne Holmes [mailto:dhol...@anatomy.umsmed.edu] Sent:

histonet@lists.utsouthwestern.edu

We are a large Mohs clinic (at least 50 mohs flats today, not counting verticals) We use Carazzi's Hematoxylin and the Eosin kit from Newcomer Supply. We use a linear stainer and it takes about 15-20 minutes. We don'rt fix in NBF, just alcohol. If you're interested, let me know. Claire

Re: [Histonet] Storage of tissues in PFA before dehydration and embedding

We fix our embryos overnight in 4% para for in situ's--the long crosslinking helps fix the RNA better. For some immuno's though, too much fixing is definitely bad. Why not store in PBS until you're ready to dehydrate? Oh, (a note of caution?), we don't use para fixation for paraffin embedding, th

[Histonet] Histology Manager for Clinical Lab in Connecticut

Our client is a Connecticut-based clinical laboratory currently seeking a Laboratory Manager to oversee the operations of several departments within the Histology Lab. This individual will manage 50+ staff in a Lean environment, and be responsible for equipment, quality assurance, expense contr

[Histonet] Storage of tissues in PFA before dehydration and embedding

Dear all, I am doing some experiments where mouse skin tissue is harvested for immuno and in situ staining at 2, 4, 6 and 8 days following a treatment. The usual protocol used in our lab (for other tissues, usually bone) is to fix overnight in 4% PFA before dehydration and paraffin embedding. As

[Histonet] Intellipath LIS

Hi everyone, Are any of you using the Intellipath LIS? Not the Intellipath stainer from Biocare. If so, I have some questions on how you use the procedures report to get your orders. Thanks! Erin Martin UCSF Dermatopathology ___ Histonet mailing li

RE: [Histonet] microtome calibration

Have you tried altering the angle of the knife? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential an