I think many, many labs perfuse animals under deep anesthesia. Notice the word
"DEEP". It is hard to judge whether the animal can recover. But we know not
because it is an overdose injection.
Then, when you write papers up, you never use "Deep anesthesia", you use
"sacrifice". That is some back
let me say like this
during the overdose injection, look into the animals' eyes.
take their soul away. then the pre-mortem perfusion is humanized.
2009-07-01
TF
发件人： Chana de Wolf
发送时间： 2009-07-01 09:30:01
收件人： Ingles Claire
抄送： histonet
主题： Re: [Histonet] Good Perfusion...
Actua
Pale liver means the blood has been removed from liver, not necessarily any
other tissues of interest.
For brain perfusion, many people will close descending atery.
The lung filling up of liquid is because you did not insert needle into the
main artery. It is small, but can be done with proper s
I think "good" for perfusion/fixation is different upon request, the antigen in
IHC, e.g.
For good histology and fixative-resistent antigens, people try to fix the
tissue extensively, to have good tissue quality and the animal goes a stiff.
For mild-resistent antigens, we never post-fix the tissu
I tried Zinc fixative I made myself.
Does not work at all? And, the tissue quality is very bad.
Any comments?
2009-07-01
TF
发件人： gayle callis
发送时间： 2009-06-30 23:57:35
收件人： 'Histonet'
抄送：
主题： [Histonet] RE: mouse brain fixation Attn: Annette Featherstone
Annette,
You did not say
Actually, as I explained, in the case of perfusion fixation, you can *and*
you should begin perfusion pre-mortem if you wish to measure anything other
than ischemic injury. That's pretty much the point.
Your own personal feelings aside, it is extremely important that people on
this list who are no
Sorry, but it is against my personal ethics (and/or morals?) to do such a
thing. Guess I'll never get to work in research... But then I can't even pith a
frog without squirming. After it's dead though, nothing bothers me. (OK, maybe
eyes) Perhaps I'm just wierd. (oh, wait, I work in a lab...) L
I stand correct on the perfusion under anesthesia issue.
Jerry Ricks
Research Scientist
University of Washington
Department of Pathology
Date: Tue, 30 Jun 2009 12:37:20 -0700
Subject: Re: [Histonet] Good Perfusion...
From: chana.de.w...@gmail.com
To: jf...@gladstone.ucsf.edu
CC: rosenfeld...@ho
Histonetters,
Perfusion under deep anesthesia is most certainly not unethical NOR illegal,
and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion
and fixation -- the intracellular ischemic cascade begins immediately upon
circulatory arrest, setting off a chain of events highl
Hi histo world..
I hate to be stupid, but if you'd have dealt with the cable TV company
today like I didyour mind would be blank tooBut now I'm back to
work, after wasting 5 hours and I STILL don't have internet, phone or
TV. I don't think I can handle one more day of this...I'm going
Dear Jerry and histonetters,
I don't believe this is unethical or illegal. It is written into our IACUC
approved protocols as follows:
Chemical Method of Euthanasia: "Perfusion under general anesthesia, Avertin
or Halothane induced. Bilateral thoracotomy."
Could it only be forbidden by your f
Steve
You need to keep you reagent buckets filled to the middle line. The 100%
alcohol should be to the maximum line especially after the eosin. You have
some running of solution down the top of the slide causing uneven staining.
When we hand stain we move the baskets vigorously up and down. That
No, I wouldn't nick the atrium.
Instead, cut the femoral artery at the groin.
And of course, you cannot perfuse a living, anesthetized animal. That would be
unethical and also illegal.
Jerry Ricks
Research Scientist
University of Washington
Department of Pathology
___
Thanks to all who replied to my inquiry on used equipment dealers. I have
received good information.
Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA
This e-mail message, including any attachments, is for the sole use of the
intended recipients and may
Annette,
You did not say what immunos e.g. antigens you are trying to stain? Nor
how you are doing the actual staining method? More information would help
please.
If you are trying to stain for murine CD markers or some other cellular
antigens, there are not many that work after FFPE.
Several of our labs perfuse mice via heart (using a smaller gauge needle and
syringe filled with fixative, or with larger rodents, via a peristolic(sp?)
pump with PBS perfused first followed by a fixative. The animals are
anesthetized via an injection first, then perfused. The perfusion itself is
Hi histonetters,
Well, I just acquire a Leica SM2500S polycut heavy duty microtome and
could use your help. Everything seems to be working however I cannot get
the knife holder to automatically descend to account for the desired
micron setting in either the manual or automatic modes. The knife
i think it probably would be better; but good luck getting approval to do
that.
--On Tuesday, June 30, 2009 9:32 AM +0530 raghul
wrote:
Dear Dzung,
I wonder whether the perfusion would be good in an euthanized animal with
the circulation arrested. It would be better to try it under deep
a
We have been working with mouse brains that were fixed in 10% formalin for
one month. We are currently getting "no" staining with our immunos. Can this
really be the problem in light of antigen retrieval methods?
Annette Featherstone
___
Histonet
but he's not using fixative.
--On Tuesday, June 30, 2009 10:06 AM +0100 "Edwards, R.E."
wrote:
A stiff tail!.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thach,
Dzung (NIH/NIAID) [E] Sent: 29 Jun
This list should really consider levying a fee of $1 on anyone who
writes the entire list asking how to unsubscribe. The list moderator
would be rich in no time :-0
Melissa Phelan wrote:
Hello,
I am trying to unsubscribe from here, but I have click on the unsubscribe
button on the website and
Hello,
I am trying to unsubscribe from here, but I have click on the unsubscribe
button on the website and have yet to receive the email to unsubscribe.
Please unsubscribe me from the list. Thank you!
--
Melissa Phelan
Laboratory/Pathology/Biotech
Cell: 407-697-1175
UCF KNIGHTS!!
Greetings all-
Thank you to everyone who sent me a copy of the CAP checklist. I found a
couple more standards I would love to get my hands on (you have to pay for
them). They are:
CLSI HS 1-A2
ISO 9001
ISO 15189
AABB-Quality System Essentials
I have learned a lot in my quest for all this inf
That's cruel; how would you like it?
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah
Lehrer
This e-mail is confidential and privile
A stiff tail!.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thach, Dzung
(NIH/NIAID) [E]
Sent: 29 June 2009 16:31
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Signs of good perfusion
Hi E
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