What is your tissue of interest? Why not do the Von Kossa stain first
and then counterstain with MacNeal's tetrachrome. This way you employ
the use of a metachromatic stain for the rest of the tissue instead of
just a nuclear staining hematoxylin.
Jack
On Jul 16, 2009, at 9:34 PM, "karine
Karine,
Either Hx will do, though I would not have used a haematoxylin since it
will lake with the calcium forming a blue stained deposit. I would
expect it to mask the silver of the von-kossa stain.
I would recommend 1% neutral red, ethylene green or even a light eosin
counterstain.
The silver "
Hi,
When doing a VonKossa stain in order to demonstrate calcium in tissue,
does it matter much if I use Mayer's hematoxylin instead of Ehrlich's
hematoxylin (which takes 6 months to ripen) ?
Also, can I simply use homemade scott's tapwater for blueing instead of
using a lithium carbonate soluti
I also run a derm lab where we gross and write cassettes. The doctors medical
assistants make mistakes every week so I set up a double checking system where
one tech accessions and check numbers and writes slides. Then I gross and make
one final qc check. this is only possible since I have low
Tony,
LOL, someone with a funny accent in Alabama. Something only a local can
appreciate.
Victor
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-
Hi Carrie,
The NSH convention in Alabama will also be my first.
It has only taken me 30 years to finally get to one.
Assuming I don't get lost I hope to see you all there (?turn right at
Honolulu, left at Los Angeles, then second exit on the left?)
I'll be the short tubby man with the funny acce
There are no acceptable "standards" for mistakes. The present tendency of
implementing the "6σ method" in the lab is to precisely eliminate mistakes, not
to set an "acceptable" limit.
René J.
--- On Thu, 7/16/09, kristen arvidson wrote:
From: kristen arvidson
Subject: [Histonet] Quality Stuf
Although I really like MOPEK, another source for the East Coast would be TBJ.
From: Golden State Acrylic Designs
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, July 16, 2009 9:34:56 AM
Subject: [Histonet] Biological hood with grossing station
Is the a s
There is NO margin of error acceptable in mislabeling blocks or slides. I
expect 100% compliance with this in my department. When you have like specimens
all day like derm, you cannot make labeling errors.
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:
Hello,
I work in a derm lab and we do all the grossing. We hand write on all of our
blocks and slides, so you can imagine we have mislabelings from time-to-time.
I was wondering if other labs have acceptable limits set for errors such as
these, and if so what are they like? I am working on set
Hi Jan,
Thanks for your input! I have two A. S. degrees. One in veterinary
nursing/technology and one in histology. And I have an AA where all my
electives were biology,chemistry and micro. Then I'll have a BS in veterinary
nursing/management. I'm starting a molecular program in January. So, I
Thanks to everyone who took the time to answer. I will explore this through
NSH and ASCP. It sound like certification and continuing education are the
keys!!
Cheers,
Loralei
On Thu, Jul 16, 2009 at 5:33 AM, Haynes, MaryAnne wrote:
> Loralei & Anthony,
> It has been a long time since I took my H
Hi Ian,
For paraffin wax infiltration, the normally quoted range of vacuum pressures
to use varies between 360 mmHg(48 kPa) and 260 mmHg(34.66 kPa).
I always reduced the pressure slowly in the first wax to just 360 mmHg to
prevent violent 'bumping' due to rapid outgassing of the clearant.
Seco
Will you be a 1st time attendee to the NSH Symposium/Convention in
Birmingham, Alabama in October? Or have not attended an NSH S/C in at
least 5 years? If so - you may be eligible for $500 to attend the
symposium and be the recipient of one of nine $500 Newcomer-Newcomer
Awards.
The 4 requireme
I recently received a request from our Cancer Center to provide whole
mount evaluations of rectal cancer specimens using the "Quirke
technique" and a sledge microtome. The articles I located describe in
depth the role of the surgeon, pathologist and radiologist. However
there was almost no informat
there are a few out there - off the top of my head id say try MOPEC
Annieinarabia - yayyy its weekend
2009/7/16 Golden State Acrylic Designs
> Is the a source for a biological hood with grossing station othe than
> (Thermo-Fisher)
> Thanks
> ___
> Hist
We have recently bought a new vacuum embedding oven but there is
some dispute in the lab as to the correct vacuum pressure we should be
using. I won't prejudice the replies by giving you our values, but what are
the common vacuum pressures that are being used?
Ian.
Dr. Ian Mont
GFP is not destroyed in frozen sections but the tissue should be PFA fixed
prior to sectioning, ideally.
If you have already prepped your tissues and have no choice but to proceed with
fresh frozen be aware that GFP is soluble and can leach out of fresh frozens.
In addition, in fresh frozen pr
Thank all of you who posted a response to my question about BS degree's
and Histotechnologist. I especially appreciate the number of responses
that came from "seasoned" HTL's. Because of your impute I was able to
convince my hospital and craft a job description that leaves no one out
from conside
Is the a source for a biological hood with grossing station othe than
(Thermo-Fisher)
Thanks
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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Good Morning Histonet,
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We use Premiere Charged Slides purchased from Cardinal and/or Mercedes for our
immuno stains. We recently purchased the Ventana Ultra and really like it. We
dual mount the control and patient. We have recently had several cases where
the control was positive but the patient was not. We repea
Hey Histonetters,
Can anybody tell me a fluorescence stain for osteoid.
I need to measure the osteoid volume around/at the surface of
bone lacunae with confocal laser scanning microscopy.
I heard about basic fuchsin but there the lacunae voids are
also stained so i guess it's hard then to dist
Hi all,
I spent hours and looked at several antibodies on this subject.
Does anybody have a protocol that actually works for GFP staining on
fresh frozen samples or is the signal simply destroyed?
Many thanks
This email is confiden
I have no scientific experience with this, but in my opinion it has to make
a difference, if the proteins are crosslinked with single methylen-bridges
or with "longer" bridges, that result of "more-C-compounds".
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.
Sub optimal fixation and as Rene said; drying off at high temperatures.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: 15 July 2009 20:45
To: Histonet
Subject: [Histonet] nuclear bub
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