[Histonet] Janella Seaton/WLGORE is out of the office.

I will be out of the office starting 08/10/2009 and will not return until 08/13/2009. I will respond to your message when I return. Any histology-related requests or questions can be sent to: histol...@wlgore.com. ___ Histonet mailing l

[Histonet] marilyn weiss will be out of the office

I will be out of the office starting 08/10/2009 and will not return until 08/11/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell or Laurie at 619-528-6801 if this is urgent they can contact me or give you my cell phone number.

Re: [Histonet] CD31 working protocol

Hi Patricia, I use an antibody from Spring Biosciences Cat#M3384 to stain our human xenograft tumors as well as human tissue. Antibody will also stain mouse tissue. Very nice clean crisp stain. At least when we use it on our Bond IHC stainiers. Thanks and let me know if you need more information

[Histonet] Cryostat repair/maintenance

Dies anyone know of a reputable company that repairs/maintains cryostat microtomes in South Florida (Miami area)? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Specimen Release

Laurie, I can fax you a copy of a release form if you want. Garret Miyamoto Tripler Army Medical Center - Original Message - From: histonet-requ...@lists.utsouthwestern.edu Date: Saturday, August 8, 2009 7:03 am Subject: Histonet Digest, Vol 69, Issue 8 To: histonet@lists.utsouthwest

[Histonet] cross contamination

Hi Histonetters- Our laboratory does not process gynecologic cytology specimens and we have a relatively small number of non gynecologic specimens. Usually when we process the non gynecologic cytology specimens, they are done separately. I saw a CAP question which addresses prevention of cross

Re: [Histonet] CD31 working protocol

Hi Patricia,   What tissue (animal / human) are you using?  How has it been fixed /  processed?   If you are using 10%NBF fixed, paraffin-embedded mouse tissue, you can use Biocare's CD31 with a simple indirect protocol.  I come in with my primary (overnight at 5ug/ml) after Prot. K digestion (DA

Re: [Histonet] CD31 working protocol

Biocare has a CD31 anti mouse that does stain FFPE.I personally think nothing will ever compare with the Santa Cruz goat that no longer is available - but Biocare does work. "Zerfas, Patricia (NIH/OD/ORS) [E]" Sent by: histonet-boun...@lists.utsouthwestern.edu 08/10/2009 01:50 PM To "

Re: [Histonet] CD31 working protocol

Biocare has a great CD31 for mouse and rat too. You do need the kit and their primary but you can use formalin fixed samples with great results! Colleen Forster U of MN Amy Porter wrote: BD Biosciences has a great rodent CD31 for mouse and rat - however you need to use their Zinc Fixative. I

Re: [Histonet] CD31 working protocol

BD Biosciences has a great rodent CD31 for mouse and rat - however you need to use their Zinc Fixative. It is well worth the results!! Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320

[Histonet] CD31 working protocol

Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Buildi

[Histonet] Re: Human samples in university research lab

Denise, You will need to contact your Institute Biosafety Committee (IBC) before you can start working on human samples in your lab. They should have everything you'll need to do in order to become compliant with federal regulations involving the use of human materials. It takes a ton of paperw

[Histonet] RE: CAP

-- Message: 6 Date: Mon, 10 Aug 2009 09:05:44 -0500 From: Vacca Jessica Subject: [Histonet] CAP ? To: "Histonet@lists.utsouthwestern.edu" Message-ID: <938d716cd445614abbb817517557b6f4c8476...@nadcwpmsgcms09.hca.corpad.net> Content-Type: text

Re: [Histonet] Fixation of embryos

Use Bouin's fixative. René J. --- On Mon, 8/10/09, Cheryl Crowder wrote: From: Cheryl Crowder Subject: [Histonet] Fixation of embryos To: "Histonet" Date: Monday, August 10, 2009, 11:27 AM Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.  After processing they are mu

[Histonet] Microm microtome maintenance in AZ

We are looking for someone to service our Microm microtomes in Tucson, AZ. Any ideas would be greatly appreciated. Bridget Maryott Advanced Staining Ventana Medical Systems, Inc. a member of the Roche Group 1910 E. Innovation Park Drive Tucson, Arizona 85755 Tel: +1 520 229 4022 mailto: bridg

Re: [Histonet] Fixation of embryos

If the G in 4F1G is glutaraldehyde the glut. will probably kill off the immunoreactivity (depending on the antigen in question). How long are the embryos being fixed? Remember, formalin works slowly so 5-7 days may be necessary.- I would try a formalin + alcohol mix or formalin-alcohol-acetic ac

RE: [Histonet] CAP ? (UNCLASSIFIED)

Classification: UNCLASSIFIED Caveats: NONE We have a MSDS for all of our chemicals. The information is listed in the MSDS. We keep copies in our MSDS Books in the lab for fast and easy access. The MSDS's are updated yearly, when we receive new chemicals or the manufacturer sends us their lat

[Histonet] Fixation of embryos

Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF. After processing they are mushy and almost amorphous. Has anyone any suggestions for a fixative that will give the embryos more form and still have the tissue capable of doing IHC? I had thought of 4F1G. Any help is re

RE: [Histonet] Good Fibrin stain with Movat's?

Judy: Amos has a good suggestion. Below is a link to a Movat procedure from Penn, we discontinued our clinical Movat procedure due to lack of use (though it is a great stain). In the attached link, note the comments about the wash and differentiation steps. The Movat is nice in that it allows y

[Histonet] e-mail update

How do I update my e-mail address? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] CAP ?

Most of that info should be available on the MSDS. Ours are available on the hospital network but we used to just keep a binder. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Messa

[Histonet] CAP ?

How does one gather this information in the lab? What are your steps? GEN.70550 "Is there documentation that each of the chemicals in the laboratory has been evaluated for carcinogenic potential, reproductive toxicity, and acute toxicity; and does the policies and procedure manual define specif

[Histonet] Human samples in university research lab

Hi all, We are a research core facility processing animal tissues for cancer research. We have been approached by a collaborator about bringing human sample to us for processing, sectioning, and routine H&E staining, for use in research, not diagnosis. In the past, we have always encour

RE: [Histonet] Mouse Vs. Rabbit

Delia: Here is a link to a good overview article for the issues involved in your question. In the early days (and still somewhat true), the main difference between rabbit and mouse derived antibodies was polyclonal vs monoclonal. Today that distinction has been blurred somewhat as there are p