Re: [Histonet] Caspase 3 staining in frozen tissue

acetone fixed -> PFA fixation again? I think one step is enough 2009-08-26 TF 发件人： lau...@conxis.com 发送时间： 2009-08-26 03:04:41 收件人： histonet@lists.utsouthwestern.edu 抄送： 主题： [Histonet] Caspase 3 staining in frozen tissue Hello everyone, Is there anyone out there doing Caspase 3 IHC

RE: [Histonet] RE: Trouble making a good Cell Block

Maria, I did not know whether you had many responses. It seems that our Histonetters are forgetting to "Reply to all" so that their valued knowledge is made available to all of us. Please everyone, reply to all not just the sender Thanks Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys,

RE: [Histonet] AB&T systems

Donna, if you're looking at LIS vendors, which I assume you are, since you're looking at Cerner's Copath, may I ask you to consider our information system. From reading through the literature, we may have been the first commercially available, vendor supplied information system that included compl

Re: [Histonet] thermometer vendor

We ordered ours through Fisher, though I'm not sure how short you need them to be. I remember it took a while to find them on the site, though, since I didn't have the exact length to put in their search engine. Try this though *http://tinyurl.com/mlk3ho* Emily "One of the defining characteristi

[Histonet] RE: Trouble making a good Cell Block

THANK YOU to everyone out there! There were so many responses; I would have been typing my 'thank yous' all day. You can use my AOL account to send me individual messages/protocols. I do appreciate it! Kind regards, Maria Katleba HT(ASCP) MS Pathology Dept. Napa CA Notice from St. Josep

RE: [Histonet] AB&T systems

There are several ones that are out there, Ventana-vantage, Dako-TPID, Cerner's solution, There is also Omintrax, but the majority of the solutions are primarly barcode with some tracking feature.. I would ask you if you have an LIS to communicate see if they would interface to a third party vendor

[Histonet] thermometer vendor

Can anyone help me find where to order the short thermometers for use in the floatation baths? Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in t

[Histonet] AB&T systems

Hello, We are investigating purchasing an Automated Bar Code and Tracking System. So far we're looking at Ventana's Vantage system, and Cerner CoPath's system. Is anyone aware of other systems out there? We're not looking for just the bar-coding, we also want the tracking features. Thanks

[Histonet] Histogel

Hello everyone! If you are using Histogel for your cytology cell blocks, how do you liquify it? Do you use an incubator or microwave? How happy are the pathologists with the quality of the cell blocks? Thanks in advance for your information. Carol Bryant, CT (ASCP) Cytology/Histology Manager

Re: [Histonet] (no subject)

Jupiter's thunder, don't you people ever learn--unsubscribe yourself in the url at the bottom of histonet emails Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Tue, Aug 25, 2009

[Histonet] (no subject)

please take me off list ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Question about Ventana IHC stainers

Hello Histonetters, I would like to know if those of you who have a Ventana Benchmark, Ultra, or Discovery have ever tried to restain slides on the machine and had a problem with inconsistent staining pattern the second time. I have found that multiple sections on a slide do not all stain the s

[Histonet] bubbles under coverslip

Hi, all Recently we met a problem of bubbles under coverslip after we changed to Xylene Substitute(from Shandon) instead of Xylene. The bubbles immediately appear after coverslipping. At very beginning, we thought it was due to mounting medium. So we changed to Shandon Xylene Substitue Mount

[Histonet] bubbles under coverslip

Hi, all Recently we met a problem of bubbles under coverslip after we changed to Xylene Substitute(from Shandon) instead of Xylene. The bubbles immediately appear after coverslipping. At very beginning, we thought it was due to mounting medium. So we changed to Shandon Xylene Substitue Mount

[Histonet] bubbles under coverslip

Hi, all Recently we met a problem of bubbles under coverslip after we changed to Xylene Substitute(from Shandon) instead of Xylene. The bubbles immediately appear after coverslipping. At very beginning, we thought it was due to mounting medium. So we changed to Shandon Xylene Substitue Mount

[Histonet] Caspase 3 staining in frozen tissue

Hello everyone, Is there anyone out there doing Caspase 3 IHC on frozen tissue? I am attempting to restandardize our protocol because the antibody we were using has stopped working. We are using acetone fixed frozen human tonsil. 4% PFA postfixation for 10 minutes. Using a rabbit polyclona

[Histonet] FISH probe labeling

Hi, All We are going to do FISH on mouse tissue. But we have to fluorescent label cDNA probe(~2kb in length) by ourselves because there is no commercial probe is available. Does anyone give me some suggestions? Like which company's product is suitable for labeling DNA probe? Which labeling

RE: [Histonet] Sakura Products

We have the embedding center and would highly recommend it -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Butte Sent: Tuesday, August 25, 2009 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [

Re: [Histonet] lead, barium detection

Lead and barium should end up as their insoluble phosphates in tissues fixed in phosphate-buffered formaldehyde. There are published methods (Pearse's Histochemistry 4th ed Vol 2 [1985]; also in earlier editions, not much different). According to DJ Cook "Cellular Pathology" 2nd ed. Scion 2006

[Histonet] System for Follow-up of Pathology Results

Greetings: We have formed a task force to look at "best practices" and system approaches to follow-up of pathology results. Medical errors have resulted when a physician's office has failed to follow-up on an abnormal or unexpected pathology result, or a recommendation made in a pathology repor

[Histonet] Re: Histonet Digest, Vol 69, Issue 26

This first thing I would like to know if this tissue has been through a previous pretreatment (antigen retrieval or enzyme digestion) of any kind on the first test, if so I would recommend not using the same slide again for any additional stains. Message: 11 Date: Tue, 25 Aug 2009 06:52:1

Re: [Histonet] Trouble making a good Cell Block

Hi Maria, Are you saying you are using formalin as a preservative for non-gyn's? We collect all aspirations in Plasmalyte which is an electrolytic balance for infusion. All other fluids are collected in a green top, sodium heparinized tube. Bronch specimens are sent in saline. Urine and CSF a

[Histonet] Trouble making a good Cell Block

Hi All, Can anyone give me a good protocol for making a cell block from a non-gyn fluid? (abdominal fluid, bronchial wash, pleural fluid, etc) First of all, we have switched to formalin. Yes! Formalin!! So as you would expect, no button forms. The reason? The pathologists believe the formal

[Histonet] Need equipment

I am part of the adjunct faculty at Elgin Community College in Elgin Illinois. We are looking for donations to a new histology program and would truly appreciate an embedding center or two. If you can guide me in the direction of one - or two - I'd be a  very happy camper !! We could also use a

Re: [Histonet] glyerin/phenol storage

Hi Jan: We used to use glycerine+phenol for storing slices of embalmed cadavers before embedding in clear plastic. I don't know the exact ratio but it was mostly glycerin. We did not stain but painted the nerves, blood vessels, etc as a teaching tool. As for IHC, it would depend on the fixat

[Histonet] Part Time and Full Time Positions In Florida (Melbourne)

Allied Search Partners is currently accepting resumes for our client in Melbourne, FL area. We are prescreening for the following: Position(s): Histotechnologists/Histotechnicians Shift: 1. One tech needed for Day Shift Full Time (8am-4:30pm) 2. One tech needed for Part Time Please submit resum

Re: [Histonet] Sakura Products

thumbs up for Sakura!! buy them - dont wait for any further advice annieinarabia (out of africa) 2009/8/25 Weems, Joyce > You will be very pleased with all Sakura products. Excellent company. > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta,

[Histonet] glyerin/phenol storage

I'm posting this for a colleague Does anyone have experience using glycerin/phenol as a storage medium, and if so, for how long are the tissues still good for Histology processing/staining? I assume they can't be used for IHC. Thanks in advance, Jan Shivers University of Minnesota Veterin

[Histonet] lead, barium detection

Hello Histonet, Our CSI pathologist wants to detect lead or barium in autopsy tissue already fixed in 10%NBF. Do any of you have any good staining methods you could share? thanks alot, Lynette ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Sakura Products

You will be very pleased with all Sakura products. Excellent company. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Sakura Products

Buying a Sakura instrument is a guarantee for durability and peace of mind. René J. --- On Tue, 8/25/09, Emily Butte wrote: From: Emily Butte Subject: [Histonet] Sakura Products To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 25, 2009, 8:54 AM Hi All: I'm looking to purchase equ

[Histonet] Sakura Products

Hi All: I'm looking to purchase equipment for a new lab and would like the opinion of anyone who has worked with various Sakura products before... good or bad. I would be looking to buy the following products: Tissue-Tek TEC 5 Embedding Station Accu-Cut SRM 200 Microtome Cyto-Tek Cytocentrifuge

[Histonet] Question about Ventana IHC stainers

Good Morning Histonetters, I would like to know if those of you who have a Ventana Benchmark, Ultra, or Discovery have ever tried to restain slides on the machine and had a problem with inconsistent staining pattern the second time. I have found that multiple sections on a slide do not all stai

[Histonet] RE: reduction/elimination of red cell autofluorescence

Date: Mon, 24 Aug 2009 10:58:43 -0400 From: "Thurby, Christina" Subject: [Histonet] reduction/elimination of red cell autofluorescence on FFPE sections for IF examination To: "histonet@lists.utsouthwestern.edu" Can anyone give feedback on reagents/procedures to use for immunofluorescence to red