They are both good instruments. From my experience pricing is high for the
Ventana reagents and there seems to be an increase in DAKO pricing from what I
see on the Net. Have you looked at the Leica Bond? We found that to be a good
fit for us. Started out to add it to our DAKO line, but ended up
The lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and
Dako's Autostainer Plus. We could really use feed back
from current users of both. We are having a hard time deciding between the
two, so any input would be great.
What are the Pros and Cons with both.
Thanks,
Hi All,
Well, I was given the green light:
It's a Shannon Grosslab Senior Workstation. It was delivered September
2004...stored ever since due to the fact it was too big to instal in the small
space. (Someone doesn't know how to measure!)
The item purchase price was $15,484.86 and has a curre
Is anyone out there performing IHC'S on the Benchmark XT's with Cellient block
sections successfully? If so would you please send me your protocol. I'm
currently trying them out at my lab with little success. We are
deparaffinizing, rehydrating and then fixing for 10 minutes in 10% NBF before
p
When I batted clean up and loaded the processors, the residents grossed in the
specimens and placed them in a basket in a container of formalin with a lid.
The grossing was done and the containers were kept on the downdraft table.
When it came time to load the processors I put the cassettes in
Hi Barb,
We have metal sleeves in which 18 cassettes fit standing front to back, and a
metal box with lid which we fill with formalin. This box holds 6 sleeves and as
cases are grossed, the cassettes are picked up in order (it doesn't take any
effort to do this at the time of grossing) and plac
We have 2 grossing stations, end each person places their cassettes directly
into the VIP basket. They go in numerically for the most part. The basket is
kept in a rectangular container with formalin and covered/partially covered
during the day. (You can get a container of this sort in your loca
Hi all -
For those that do batch overnight processing, how do you organize the
cassettes?
Currently we have 2 path assistants that gross throughout the day, and each
puts their cassettes as they are grossed into a bucket of formalin. At the
end of the day a histotech drains the formalin off, ri
Hi Nicole,
I do LFB with IHC on FFPE human brain but there are a few differences in
our protocols. I have stained LFB with Neurofilament and with S-100 but
never with Ki-67. I also use DAB as my chromagen and differentiate the
LFB with dilute Lithium Carbonate.
I'm not familiar with the VIP chroma
I do double HRP immunostains. Works well using DAB/AEC and True Blue
... but what are your favorite chromagens to use? Would love some
feedback for inspiration.
Andrea
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Make that interfaced!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, August 26, 2009 13:25
To: Bell, Pat; anita dudley; em...@pathlabsolutions.com;
histonet@lists.utsouthwester
Ours works very well!! Both cassette and slide printer are interfaces
with LIS.
Joyce
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell,
Pat
Sent: Wednesday, August 26, 2009 13:20
To: anita dudley; em.
Hello,
We are currently searching for a Clinical Supervisor for our growing
Immunopathology Laboratory within the Department of Pathology at Brigham and
Women's Hospital.
If you are interested, the posting is available online at
www.brighamandwomens.org. Clinical Supervisor, Immunopathology. Job
Just don't buy the slide printer.
Pat Bell, HT(ASCP)
University of Colorado Denver
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley
[azdud...@hotmail.com]
Sent: Wednesday, August 26,
I just purchased some from the VWR Catalogue, page 538, top left hand
corner, disposable and reusable, 2009-2010 catalogue and you can access it
online.
Cheers,
Maureen Bukhari
Phone: 403-210-6524
e-mail: mlbuk...@ucalgary.ca
-Original Message-
From: histonet-boun...@lists.utsouthweste
The cause of your bubble trouble is replacing xylene with a liquid whose
identity is unknown and whose physical properties are obviously different. For
an important application such as research or diagnosis it is not scientifically
or ethically justifiable to use unknown chemicals.
John Kiern
emily, we have a sakura processor, embedding center, an accu-cut SRM microtome
and a coverslipper. they are all great. you can't go wrong with sakura. anita
> Date: Tue, 25 Aug 2009 08:54:22 -0400
> From: em...@pathlabsolutions.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet
Hello,
I was wondering if those of you who are using H & E stainers with heating
capabilities/ovens could tell me what your average run time is. What instrument
are you using? and is the length of time affected by the number of racks being
stained?
Thank you,
Toni Rathborne
Pathology Supervi
Hello,
I have recently began using the Anti-NuMA primary antibody to distinguish the
human mesechymal stem cells that I am delivering to the rat heart. Anti-NuMA
recognizes human nuclear mitotic apparatus protein. I have tried two secondary
antibodies: Alexa Fluor 488 and FITC (Jackson immunore
Hello-
Has anyone tried to counterstain an IHC stain with Luxol Fast Blue? I am
worried that the LFB destain will remove some of the IHC.
I use the VIP solution from Vector as the chromogen for IHC instead of DAB and
I destain Luxol Fast Blue with Hydroquinone/Sodium Sulfite (in addition to th
Has anyone used this chromagen? I want to use it with N52.
Thanks for any help.
Ruth Yaskovich
N.I.H.
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To make a cell block from formalin-fixed cells, you need to introduce some
coagulable material that will stick the cells together. Here's a method I have
used successfully:
Spin down the cells in formalin, decant as much formalin as possible with a
pipette.
Fill tube with isotonic buffer, resu
Dako has a positive ID system as well.
Walter Benton
Histology Supervisor
University of Maryland
Anatomic Pathology
22 S. Greene St
Room NBW65
Baltimore MD 21201
(Direct) 410-328-0930
(Lab) 410-328-5524
(Fax) 410-328-5508
This e-mail and any accompanying attachments may be privileged, conf
I have used the ULYSIS Nucleic Acid Labeling Kit from Invitrogen. They make it
with many different fluorochromes, I have used the AlexaFluor 488 and
AlexaFluor 594 with good results.
Joseph A. Jurcisek
Senior Research Associate
Center for Microbial Pathogenesis
The Research Institute at Nationw
Hi Donna,
I have looked into the systems and if you want more than a bar code and
tracking system look into Vantage. It is what we chose and we are very happy
with it. It will track and trend quality issues, send special instructions
about a case to different workstations, the list goes on and
I agree; it would have been good to see some of these responses posted to
Histonet. I monitor the 'net for the purpose of learning the answers to
specific questions that others end up posting first, besides adding to my
own personal database of general valuable technical knowledge. So hitting
Emily,
We have 7 VIP5 processors, 2 new 120x express processors. 3
embedding centers, 2 microtomes and the DRS2000 H&E stainer with
attached tape coverslipper. All are awesome!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouth
we have the Embedding centre, DRS2000 stainer, 2 VIP5's, a tape
coverslipper and 2 microtomes - all fantastic, dependable, easy-to-use,
workhorses - need I say more - this is SAKURA - the name speaks for
itself!!!
2009/8/25 Bartlett, Jeanine (CDC/CCID/NCZVED)
> We have the embedding center and w
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