[Histonet] ISH and Bone Marrows question

Hi Histonetters! We have recently changed to Immunocal to decal bone marrow biopsies. Immuno stains performed on the decaled bone marrows look good. All In-situ hybridization negative controls, and Kappa and Lambda stains on decaled bone marrows have a blue artifact at the edges. We use the V

[Histonet] ImmPACT DAB kit

Has anyone tested this? I would be very interested. Ta carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] gastrocnemius muscle problems in paraffin

Hi All: I am having difficulty sectioning mouse gastro. muscle. They were brought to lab in 4-5mls(not enough fix) of 10%nbf for 3 days. Processed for over 8 hours(vacuum from 95% to Paraffin everyother station) . The tissue will not section. The white area appears once surfaced inidicatin

[Histonet] Thomas Crowell is out of the office.

I will be out of the office starting 09/15/2009 and will not return until 09/16/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. ___ Histonet mailing list Histonet@lists.utsouthwestern.ed

Fw: Re: [Histonet] antibody validation Thanks, but need Non-"U" also

Hi Cynthia, Thank you, this is exactly what I was looking for.  You are a rare jewel.  Especially for the size of your facility.  I am impressed!  I wish I would have received more feed-back from small labs, but that's OK.  I have been doing some pro-active sleuthing of local labs. After n

Re: [Histonet] mice legs

I did a lot of IHC work with whole mouse legs where we had to pay close attention to the tibia, as well as the joint.When the mice were necropsied, I had the prosectors remove all the soft tissue and foot without disrupting the joint. They could do this easily with fresh tissue as opposed

[Histonet] C3d

All, I have a request to work up a monoclonal C3d fluorescent stain (for frozen sections). Can anyone out there suggest a good one? I am currently running C4d & clinicians are asking for the C3d as well. Thank-you in Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _

Re: [Histonet] mice legs

Hi Sharon, I frequently get whole mouse legs submitted to me and decalcification doesn't break down the tissue. I use Cal-Rite as a decalcifier but any formic acid decal would be sufficient. Since formic acid is pretty forgiving, I decal the legs for 48-72 hours on a rotator. I change the solu

[Histonet] mice legs

Good Morning Histo World, I would like to know if anyone is working with mice legs, I have a PI that I work with that wants to process the whole leg, the problem is I need to decal it first and is wondering if the decal will break down the tissue, I think it would he doesn't think so. And if an

[Histonet] CPT technical codes for FISH testing

What CPT codes are people using for technical billing for FISH testing performed on paraffin sections (e.g., HER2)? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospita