Hi Everyone,
One of our pathologist has some IHC results that is a bit confusing...
Patient had previous Squamous cell Ca of the lung... had long courses of
radiation and Chemo...eventually died...
At autopsy, the tumor looks much more primitive and bizarre than
before... radiation effect, et
I have only done conjugation of biotin, HRP, and Alk-Phos.
But I have discovered a new kit that makes the labeling process a snap. It is
the "Lynx" kit from Serotec. They also have fluorophore labeling kits. It is
fast, easy and very efficient.
Jerry Ricks
Research Scientist
University of
Hello fellow Histonetters!
I was wondering if any of you have ever labeled an antibody with fluorescence.
If so which kit and protocol did you use? Or did you send the antibody out to
be labeled? I'm hoping that you guys can help me out yet once again! Thank
you all so much in advance. I r
Lynette Pavelich sent me a procedure. My pathologist doesn't want to do
it, he thinks that somewhere in this country there is someone who does
it. Lynette is checking to see if they can.
Thanks to everyone!
From: Jackie M O'Connor [mailto:Jackie.O'con...@abbot
When I was in Honolulu, loaded with shipyards - we frequently digested
lung tissue in sodium hypochlorite (bleach) to get an aggregate of
asbestos fibers (if they were present). I don't recall the exact
procedure, but it was quite common then. We were requested to do this by
lawyers, mostly.
Super Mega cassettes are from Thermo Scientific
Ben.
No trees were hurt in the sending of this email however many electrons were
severely inconvenienced!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf O
Try Super Mega Cassettes. They are 72mmx52mmx17mm.
Ben.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, September 22, 2009 12:51 PM
To: Laurie Colbert; Histonet
Subject: Re
IN THE PAST WE HAVE USED A FITE'S STAIN FOR STAINING NOCARDIA. THE OLD
PROCEDURE WE HAVE USES A XYLENE-PEANUT OIL MIXTURE OR SOLUTION. WE ARE TRYING
TO COMPLETELY RID OURSELVES OF XYLENE AND TRIED THE STAIN USING A XYLENE
SUBSTITUTE. WE DIDN'T HAVE MUCH LUCK. DOES ANYBODY HAVE ANY ADVICE
Joyce
Off the top of my head , could you not digest tissue in a tube with
proteinases, spin down and then examine any sediment?
Barry
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Tues
Just trying to help a patient. I have a patient who wants to know if her
lung adenoca could be tested to see if any asbestos could be involved.
One of my pathologists suggested I call Mayo. Nothing there. He said
someone used to do the digestion procedure at SUNY. So I asked the
experts. I have a p
Mercedes Medical has them.
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histon
Joyce
Why do you want to dissolve these?
I seem to remember that these are usually characteristic shaped structures that
are iron positive and should be easy to identify on morphology alone or am I
mistaken?
Barry
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailt
Sakura carries them.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of maureen
bukhari
Sent: Tuesday, Sept
Does anyone out there know where I can find a fridge-tray or a cold plate to
cool wax blocks while cutting
Maureen Bukhari
Phone: 403-210-6524
e-mail: mlbuk...@ucalgary.ca
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Vector
From: Thomas Pier
To: mark.elli...@hli.ubc.ca; histonet@lists.utsouthwestern.edu
Sent: Tuesday, September 22, 2009 12:37:56 PM
Subject: Re: [Histonet] MOM Kits
Mark,
I've had very good results with Biocare Medical's MOM polymer detection kits.
Tom P
I use the MM Biotinylation Kit from Biocare and it's great as are all their
products.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Tuesday, September 22, 2009 12:21 PM
To: histonet@l
Mark,
I've had very good results with Biocare Medical's MOM polymer detection kits.
Tom Pier
>>> "Mark Elliott" 09/22/09 12:25 PM >>>
Hi All
Someone has asked me about MOM kists and since I don't do mouse work I thought
I would ask the experts. What is the best MOM kit available for use with
Laurie,
Perhaps check out Electron Microscopy Sciences - autopsy capsule...a 2
part round, perforated stainless steel cassette about 3" in diameter and
0.5" deep. I have used them for processing monkey and canine brains.
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO
Hi All
Someone has asked me about MOM kists and since I don't do mouse work I thought
I would ask the experts. What is the best MOM kit available for use with FFPE
mouse tisseus?
Thanks
Mark
***CONFIDENTIALITY NOTICE***
This electronic message and any attachments are intended only for the us
Marcia Fisher at El Centro [CA] Regional Medical Center asks:
>>Does anyone know where to purchase the dye Night Blue? I used this stain for
>>AFB many years ago (1986) and would like to introduce it here.<<
I don't know whether it's still available. Victoria Blue R was used
for this same purpos
Laurie, I have cassettes that are 3x2x.75 in dimension. Call me to discuss how
many you may need.
Jerry Helisek
US Laboratory Supplies, LLC
919-264-7964
je...@uslabsupplies.com
Message: 15
Date: Tue, 22 Sep 2009 08:59:40 -0700
From: "Laurie Colbert"
Subject: [Histonet] Large cassettes
To:
Me
Hi,
I process large portions of tissue and eyes. There are no cassettes as large as
4"x4". Believe me I have been looking for 30 years. But, what I use are soap
molds, plastic tubs, L-blocks, etc.
Paula
From: Laurie Colbert
To: histonet@lists.utsouthwestern
I've attached our protocol that we have successfully used in the past.
Prior to using this procedure, I would deparaffinize your block, taking
it down to water.
Hope this helped,
Lynette
Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley P
Hello HistoNetters,
I have some equipment for sale. I have one Ventana Benchmark XT with
three Nexus IHC stainers for sale. I have a number of Sakura Tissue
Storage Cabinets for sale. Finally, I have an Epson LQ-590 printer for
sale. If anyone is interested, please see our web site at
http://ww
My pathologist wants to sample a particular feature of a baby's brain
(autopsy), but the brain is too soft to cut and still maintain this
feature. She wants to process a very large section of the brain, and
then cut it down after it has been processed and has some firmness to
it. Does anyone know
Does anyone know about a digestion procedure for asbestos bodies on FFPE
lung tissue? Our pathologist remembers that someone used to do this at
SUNY. I'd appreciate your feedback.
Thanks,
Joyce
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA
Hello
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Start of October I'm giving a lecture on the enteric nervous
system and I'm short of a few images. I've got beautiful micrographs of the
neurones stained with silver and conventional stains, but lack some IHC of
various neuropeptides either in sections or 'en face' As it's the start of
Histo-netters? Looking for the best method to process some mouse eyes in
paraffin. Let me know about the fix, too...(.if it is something exotic?)
I have a project coming my wayand I am sure there is someone out
there who has done this a million times...and I don't need to re-invent
the wheel fo
If its happening to an entire batch but not to other batches then you
can sorta rule out fixation unless you use different batches of fixative
for different batches of samples; logical yes? To put it another way if
you use the same fixative and fixation time on all batches but only one
is affected
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