Hi all,
I have a few antibodies that I have successfully gotten to work using
immunohistochemistry (primary + biotinylated secondary + SA-HRP + DAB). I am
hoping to move from IHC to immunofluorescence. I tried the same staining
using either a fluorescently conjugated secondary or a biotinylated
se
I need advice from you wonderful techs about what processor would be best
for a Derm Lab. I am going to the NSH next week and with your advice I will
be able to look at some. I work now in a hospital and we have 2 VIP (Sakura)
processors. I like them very much. But for a derm lab would some of
Dr. Richard Cartun,
Our laboratory offers MSI testing and typically do go thru a Genetic Counselor
first.
Thanks,
Milton A. Gomez, HTL (ASCP)
Technical Supervisor
Immunohistochemistry Department
ARUP Laboratories, Inc.
500 Chipeta Way
Salt Lake City, UT 84108-1221
Desk Phone: 801-583-2787,
To All,
Gudrun, thank you for the references plus I liked your protocol where the BM
are well fixed before decalcification. If anyone is interested I have a
simple decalcification endpoint check that works as long as you have a
balance that weighs in mg to three places. We use this faithfully f
I agree with Gayle, that EDTA is the best way for preserving DNA and RNA.
But we have switched to formic acid decal of bonemarrow biopsies for the
speed-reason. And we have found that IHC has become better with some
markers.
This year I tested CISH (Kappa/Lambda) on 25 BM and compared the results
