You can leave it in formalin for 48-72 hours without a problem, it might be
better than fixing just for 4 hours, some say adequate formalin fixation
takes at least 24 hours no matter the size of the sample.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora
This would work but make sure your tissue is very well fixed 24-48hrs before
letting them sit in 70% or they will get alcohol fixed and that can be a
problem.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.or
Are they cutting the pieces extra thick because they are sitting over the
weekend, perhaps? Even if the tissues are well fixed if they are too thick
they will not process well, especially breast fat.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax
I will be out of the office starting 11/24/2009 and will not return until
11/30/2009.
I will be on vacation until Monday, November 30th. I will respond to your
email when I return.
__
This email has been scanned by the Message
hi Robin-
IThere are a dozen ways to address this but a very simple one without extra
resources might be this:
Set your processor with the extra 24-48 hours held in the first alcohol. You
probably don't want to spread the time across the whole machine or your end
product will be VERY differe
Yes! Heaven forbid we don't need any dim bulbs in the lab. :)
Sorry couldn't resist. Happy Turkey day everyone.
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of alan taylor
Sent: Wed 11/25/2009 8:37 AM
To: jstaruk; histonet@lists.utsouthweste
Kris,
why don't you leave the skin biopsies in NBF over night?
At our institute it is usual practice to let this specimens fix until next
day, if they arive after 1 p.m. I cannot see a negative effect.
For me 4 hours in formalin are too less for a good fixation. How long is the
duration of fixatio
We don't work on Saturdays, either. Our pathologists come in on Sunday
to gross, so they take the breast specimens off of the processor and
place them in a plastic container. On Monday morning, we melt them down
and embed and cut them.
We have three processors and one is set up for breast and/or
I keep hearing that the "guidelines" will be changed to "6-72 hours".
Therefore, I wouldn't make any changes right now unless you are getting
suboptimal results.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director,
Side note question - How are you dealing with the timing issues with
breast specimens being fixed in formalin for greater than 48 hr in
regard to her-2 requirements with not working Saturdays?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists
70% ethanol is the best solution for any kind of long term post-fixation
storage of tissue samples. Tissues can be left in it essentially indefinitely.
PBS has no preservative properties, doesn't prevent growth of microorganisms,
and should be used for short term storage only, and then only with
I'd be curious to see how you have your processor times set for each
station. I'm sure you're using a different setting on the weekends to
account for the longer time. Have you made sure that all the other
stations are getting the same time they normally get during the week?
Drew
On Wed, Nov 25
Wanted to share this reply.
Original Message
Subject:RE: [Histonet] Sakura Embedding
Date: Wed, 25 Nov 2009 09:56:56 -0500
From: Durden, Kelley
To: 'Victor Tobias'
References:
<0c96f0bfe078d74c91a1c541d24a6ae4968cd...@emgmb1.ad.medctr.ucla.edu>
<4b0d4032.50
We have been having processing problems for a while now and cannot resolve it.
It only happens on weekends. When we embed and cut on Monday morning, alot of
our larger pieces of tissue, especially breast, are not fixed and processed
well. I would expect the opposite especially since they are in
That was my concern...leaving the orientation of tissues up to grossing
personnel, residents and other unsavory folks doesn't sound like a wise idea to
me
Noelle
-Original Message-
From: Victor Tobias [mailto:vic...@pathology.washington.edu]
Sent: Wednesday, November 25, 2009 6:33
Hi Jim
Although we are based in the UK we obtain our replacement bulbs from a
company in Chicago called Topbulb. They have a huge range of lamps and bulbs
for microscopes, lab equipment and medical equipment. Their prices are
competitive and they offer a quick service. I'm sure that if you gav
As I recall you don't have to do anything special at the time of
embedding, but what is it like for the person grossing? They have to
correctly orientate the tissue or the specimen could be ruined?
Victor
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept
Same here- we use Abcam also.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@list
i am - love it!!
2009/11/25 Linke, Noelle
> Is anyone using the Sakura TissueTek AutoTec automated embedding machine in
> their lab? Do you like it?
>
> Thank you!
> Noelle
>
> Noёlle Linke M.S., HTL(ASCP)QIHC
> Manager, Histology Services
> Department of Pathology & Laboratory Medicine
> David
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