Kristopher:
We did and still does next things:
1. Fixation in 10% NBF not less than 24 hours at RT
or at 37-40oC overnight (from 16 PM to 8 AM).
2. Rinse with tap water 15-20 mins at RT.
3. Keep in 70% isopropanol at RT before processing
as need long without any adverse effects.
We totally exclude
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Are you already certified to practice in Canada? If not, you will need to
contact the CSMLS (Canadian Society of Medical Laboratory Sciences). They can
guide you on how to go about getting Canadian certification (which you must
have to practice in Canada). If y
Very surprising!
I just use PFA fixation after I cut the tissue, and I can stain it again!
Anyone has the experience of staining IBA1 on alcohol/acetone-fixed brain
sections before?
In literature it said yes this works.
-- Original --
From: "TF"
Hi all, i just can not get this IBA1 (marker of microglia) well stained on my
frozen sections fixed with acetone (sections were either dried up in air for 1
hour or fixed immediately). But I always get good staining on PFA-perfused
brain tissue.
Will IBA1 antigen reactivity lose in acetone fix
Hi all,
A couple of weeks ago I posted a message about needing a new supplier
for Sanderson's RBS now that Surgipath/Leica have stopped selling it.
I've just had confirmation that Dorn & Hart Microedge, Inc in Chicago
will be taking over the manufacture and supply of Sanderson's RBS. Stain
produc
Hi,Good point you are raising here! Despite tons of literature mixing up the
terms IHC and ICC (especially coming from the UK), I do believe there is a very
big difference here. Like you described, we also encountered a situation that
IFNgamma antibody worked very well in ICC (after fixing the c
