Pam is correct, I mean, look at me, I've been denatured for many years.
Happy New Year everyone
JTT
- Original Message -
From: "Pamela Marcum"
To: "Smith Wanda"
Cc:
Sent: Monday, January 04, 2010 9:52 AM
Subject: Re: [Histonet] Problems with Denatured Alcohol?
Wanda,
The first
Allied Search Partners is currently accepting resumes in Northern New
York, just
North of Albany, NY area for qualified Histotechnicians/Histotechnologists
Sign On Bonus:
Through Allied Search Partners $2000.00
Histotechnologists/Histotechnicians
Shifts: Day Shift, 11am-7pm, Full Time, Monday-Fr
I have had the opportunity to work with the Kappa, Lamda and EBV. Could not be
easier. Tech's whom have never run an immuno by hand were doing them on the
first day.
I believe it is set to be release as an IVD or ASR very very shortly.
Any specifics contact me directly.
Loralee McMahon, HT
Hello Histonetters,
A client of mine in the Chicago area is currently hiring for a Lead
Histotechnologist. I have listed the details for this position below and
if you are interested I can be reached via email at jcraw...@aerotek.com
or by phone at (847) 221-1358.
Histotechnologist Job D
I believe a cornflake artifact is when your slides are air dried and then
coverslipped you may get little refractive tiny grey spots on the slides that
look like corn flakes (abnormal shapes).
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, C
hi all
My name is Wassan am arabic i live in Iraq ,in north of Iraq to be specefic i
greduated from scince collage/biotechnology department 2008,i work in a
histopathology department doing basic histopathology and manual
immunohistchemistry as a technician,i have avery supportive superviser who
Hello everyone,
Do any of you have experience with Rapid In Situ Hybridization. I
believe Biocare has something NEW for rapid ISH staining. Are there
other methods out there? Has anyone tried Biocare's Rapid ISH? If you
have tried it would you let us know what you think of product.
Th
We use formic acid (10%) for decalcification. We fix in 10% NBF for 48
hours. We decal for about 7 days for the mouse joints (ankles, front
and rear paws), knees do not take as long.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 8030
Dear Histonetters!
I have a project coming up which would require to decalcify mouse hind legs,
femur and tibia with joints. Can anyone suggest a good system, either an ion
exchange or acid decal(formic acid I've heard works well) and also if anyone
has a protocol on fixation and decaling, it would
I've managed to get one chemokine (SDF-1) to work on FFPE sections. The key
was using the tyramide amplification system from Perkin Elmer. It's a dirty
staining because it's secreted so you see staining bound up randomly in
noncellular extracellular matrix but you do see it concentrated in some
cel
Hi All, Happy New Year...
As the new year is upon us I and my fellow histologist feel compelled to
attempt IHC on mouse tissue again for the detection of various cytokines.
As I have not had much success in this area in the past I thought I would
ask if there is anyone out there that you know
You have to infuse the lung with a 50/50 oct sucrose solution prior to
freezing.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Meghan Tucker
Sent: Monday, January 04, 2010 9:23 AM
To: histonet@lists.uts
Hi everyone,
I hope you all had A wonderful Holiday. Does anyone have any suggestions on
how to soften teeth to cut in paraffin?
Thank you
Fawn
-
This electronic message may contain information that is
confidential or legally privileg
Don't know if anyone else replied to this...but maybe try tinkering with
your angtigen retrieval? Like try altering the temp, duration, or even the
pH? pH can be finicky for some epitopes, I've found...
Regards,
Merced
--On Wednesday, December 23, 2009 12:56 PM -0600 "Margaryan, Naira"
wrot
Wanda,
The first thing you need to find out is what did they denature it with.
Denatured alcohol may not be the mixture of isopropanol and methanol in ethanol
you were getting and could be chemically denatured.
This is a problem that comes up when we don't check all the variables in
I always perfuse/inflate my lung samples with dilute OCT before freezing. It
does make the cutting much easier and the morphology is pretty good considering
that it is a frozen section.
Good luck,
Linda
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu [mailto:histone
Cambio does sell it, I've used their probes. OpenBiosystems is their US
distributor. But it still takes 4-6 weeks (minus a hold up in customs)
just to get probes to the US...not sure about Israel.
We are looking at ordering from ID Labs, a company in London, Ontario
(Canada), which is much cl
Hi Meghan,
Healthy lung is terrible to cut frozen. All those air spaces collapse
on sectioning. Unhealthy lung on the other hand is a dream to cut! I
have heard it suggested to perfuse the lungs with dilute frozen cutting
compound (eg OCT or similar) prior to selecting the piece(s) for frozen
secti
Happy New Year to Everyone!
We recently changed from Reagent Alcohol to Denatured Alcohol as a cost saving
measure per the "bean counters". The cutting histotechs have started
complaining that the tissue seems dry and they are having difficulty cutting
sections. Has anyone else had this pr
Hello!
I am looking for some helpful hints for cutting frozen lung tissue. I have
tried just about everything I can think of!
Thanks!
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Does anyone have any protocols to comply with the new CAP regulations regardng
imaging?
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Dear Histonetters! Does somebody use polyester wax for embedding? I need it
for hydrogel that disappears in hot paraplast. Can you advice any
manufacture to me? \Thanks, Julie Carmel, Haifa, Israel
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