[histonet] Cytokeratin 17

2010-01-06 Thread Nicholas David Evans
Dear all, Can anyone suggest a decent, reliable antibody to mouse cytokeratin 17 (polyclonal or monoclonal is fine) I could buy? There are many on the market, but I want to be sure it works. Best wishes Nick ___ Histonet mailing list Histonet@li

Re: [Histonet] Policy on Floaters

2010-01-06 Thread Marcia Funk
Floaters Yes, you are so right, for patient safety and your safety, policy is a must. Protects you and the patient. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> Mark Tarango 01/06/2010 2:21 PM >>> Your goal is not to have

Re: [Histonet] Policy on Floaters

2010-01-06 Thread Rene J Buesa
You document it in the QA file and prepare a follow up in the PI program. René J. --- On Wed, 1/6/10, Bill wrote: From: Bill Subject: Re: [Histonet] Policy on Floaters To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, January 6, 2010, 3:12 PM At 12:02 PM -0800 1/6/10, Rene J Buesa wr

RE: [Histonet] Policy on Floaters

2010-01-06 Thread Terri Braud
Ok. I have to wade in. We actually have a policy on floaters. Our policy calls for the pathologist to circle the floater on the slide and mark it as a floater with a cell marking pen. That way the "floater" is identified forever on the permanent slide. He/she will notify the Supv or lead tech

[Histonet] special stains

2010-01-06 Thread Jennifer Campbell
I couldn't agree with you more, Liz! I am from a GLP lab as well and we have to undergo validation for every single new piece of equipment we acquire otherwise we are not considered GLP-compliant. Who is to say that your new stainer is going to stain slides in the same manner as your old stainer?

Re: [Histonet] Policy on Floaters

2010-01-06 Thread Mark Tarango
Your goal is not to have floaters. If you get one, your policy should set out to determine the cause of these incidents. You should track who did it (in a spreadsheet), where it happened (grossing, embedding, cutting...). Then you should have a meeting every so often with people from the lab and

RE: [Histonet] Policy on Floaters

2010-01-06 Thread Blazek, Linda
Document it and see it there is a trend. Linda B -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Wednesday, January 06, 2010 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet]

Re: [Histonet] Policy on Floaters

2010-01-06 Thread Bill
At 12:02 PM -0800 1/6/10, Rene J Buesa wrote: >It is difficult for you to find a "policy on floaters" because any lab will >try to not get any floater. >It could be said that 1 floater is 1 too many. The goal is "0" floaters. Since no one is perfect and floaters WILL happen at every lab, what do

Re: [Histonet] Policy on Floaters

2010-01-06 Thread Rene J Buesa
It is difficult for you to find a "policy on floaters" because any lab will try to not get any floater. It could be said that 1 floater is 1 too many. The goal is "0" floaters. René J. --- On Wed, 1/6/10, Cho, Soo-Jin wrote: From: Cho, Soo-Jin Subject: [Histonet] Policy on Floaters To: "histo

[Histonet] NYS Licensing Examination Update

2010-01-06 Thread NYSHisto
Hi Everyone We have excellent news..!! we have just received this update from the New York State Office of Professions regarding the examination procedures and eligibility for licensing in NYS, Please see below.  Since this is "hot off the press" information, I am sure there will be questio

[Histonet] Policy on Floaters

2010-01-06 Thread Cho, Soo-Jin
Hello, I'm a resident at UCSF currently working on a QA/QI project regarding floaters, with the ultimate goal of formulating a departmental policy regarding floaters. Despite extensive searching on the internet and the Histonet archives, I have not found any concrete examples of policies at oth

[Histonet] IHC recommendations paper - FYI

2010-01-06 Thread Liz Chlipala
A lot of people have asked me for the paper that I mentioned in my e-mail yesterday. I think I found it on the CAP website, but here is the papers title and authors. It's a review article. Its helped me a lot with IHC validation. VM114 Standardization and Troubleshooting Immunohistochemistr

RE: [Histonet] a-2 microglobulin rat

2010-01-06 Thread Liz Chlipala
We have done this before and the antibody we used came from SSI, Catalog Number: HYB 339-01. It worked fine on FFPE rat kidney samples. ilz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www

[Histonet] Peloris - xylene free

2010-01-06 Thread Houston, Ronald
Is anyone using the Peloris tissue processor with isopropanol instead of alcohol/xylene? I am interested in your experiences. We are demoing the machine just now and have been very impressed with its results using traditional processing modules. However, when we switched over to isopropano

[Histonet] α-2 microglobulin rat

2010-01-06 Thread Bruijntjes, J.P. (Joost)
Hi All Is anyone of you aware of a supplier of an antibody directed against α-2 microglobulin applicable on rat kidneys? I have found one, but is should be working in Elisa en Western blotting only, and I need it for immunohistochemistry. Joost Bruijntjes TNO Quality of Life Zeist The

[Histonet] Biogenex i6000 or ????

2010-01-06 Thread Silverman, Jeffrey
Hello Histonetters, It's been a long long time since I've subscribed, and I'm back since I need help. We currently run a Dako Artisan for our limited IHC menu and tinctorial special stains. I've been informed that Dako will stop supporting IHC on the Artisan in about 18 months. Our machine is ver

[Histonet] Referrals Needed for Staff Histology Openings in Illinois, Kansas and Oklahoma

2010-01-06 Thread Robert . Cordero
Hi, all! I wanted to check in and see if anyone was possibly interested or knew of someone who would be interested in a few day shift opportunities we had posted in Illinois, Kansas and Oklahoma? All three opportunities are offering relocation assistance, full benefits and competitive salaries.

RE: [Histonet] CISH - HER2

2010-01-06 Thread Hoekert, W.E.J.
We are using Spot Light her2 Cish from invitrogen (manual, FDA approved). Willem Hoekert Pathology OLVG, Netherlands Van: histonet-boun...@lists.utsouthwestern.edu namens Taylor, Jean Verzonden: di 5-1-2010 22:02 Aan: 'histonet@lists.utsouthwestern.edu'; 'ih..