Re: [Histonet] Counterstain for fluorescent tissue

2010-01-08 Thread John Kiernan
Dear Nick Evans, First: Say who and where you are, and who is in charge of your experiments with mice. Second: Tell your boss to buy two or three histotechnology textbooks (about $50 each) and allow himself and you and all your colleagues 30 mins paid daily reading/lunch time. John Kier

[Histonet] Positive Control Tissue for Ab

2010-01-08 Thread Marc & Sandy Shaeffer
IHCWorld.com has a list of antibodies and the positive control tissue that can be used for each antibody at: http://ihcworld.com/_technical_tips/positive_control_tissue_list.htm. I don't have any affiliation with this site or IHCWorld or antibodies or do I sell control tissue (even though this

[Histonet] Counterstain for fluorescent tissue

2010-01-08 Thread Nicholas David Evans
Dear all, I am sorry if this is a bit of a basic question. I would like to observe localization of fluorescent microparticles embedded in mouse skin. I plan to do this simply by cryosectioning skin, mounting the tissue without fixation, and observing under the fluorescence microscope (the parti

[Histonet] out of office

2010-01-08 Thread Marilyn . A . Weiss
I will be out of the office starting 01/08/2010 and will not return until 01/11/2010. .In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. ___ Histonet mailing list Histonet@lists.uts

[Histonet] RE: cassette labelers with bar codes

2010-01-08 Thread Mahoney,Janice A
>From What I have seen I would go for the newest Thermo Shandon model. Jan Mahoney Omaha, NE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Friday, January 08, 2010 2:21 PM To: histonet@

Re: [Histonet] Question about special stain controls

2010-01-08 Thread Rene J Buesa
If you process the control in any different way than the case slides, it is no longer an acceptable control for that test. Remember that the control is used to determine if the reaction took place when used simultaneously and in an identical way as the test slides. René J. --- On Fri, 1/8/10, Th

[Histonet] Question about special stain controls

2010-01-08 Thread Thurby, Christina
I have a question for you all regarding the use of special stain controls (non-IHC). I have many years of experience doing special staining and was trained to always treat the control sections the same of the patient or study sample. Does anyone have any references if it is ever appropriate to

[Histonet] cassette labelers with bar codes

2010-01-08 Thread Diana McCaig
Can anyone advise me on cassette labelers that print 2D bar codes? Preferences or suggestions what to look for. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it m

[Histonet] coding

2010-01-08 Thread Demarinis, Carolyn
What resources are labs using for pathology professional billing coding and modifier questions to assure the correct CPT codes are applied to surgical and cytology cases? This e-mail communication and any attachments may contain confidential and privileged information for the use of the designat

Re: [Histonet] shipping slides, elementary question

2010-01-08 Thread Percival Karen
Hi Nicole, I've shipped slides many times in the hinged, cardboard slide boxes. If you wrap bubble wrap around them and use extra bubble wrap or "peanuts" in the shipping container so that the boxes don't move around, you should be fine. Good luck, karen Karen Percival, BS, HT Research Scien

Re: [Histonet] shipping slides, elementary question

2010-01-08 Thread Roger Moretz
We always packed the boxes with wipe-alls to hold the slides secure when the lid was closed, then taped the boxes closed (but those were plastic boxes).  Strong rubber bands can be used with the cardboard/wood boxes to ensure that the lids do not pop open during shipping.  This was successful du

RE: [Histonet] shipping slides, elementary question

2010-01-08 Thread Rathborne, Toni
If you package the slides back-to-back in that box, they will stay more secure. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Nicole Collette Sent: Friday, January 08, 2010 1:29 PM To: histonet@lists.utsout

[Histonet] Poor Nuclear Detail

2010-01-08 Thread Rae Staskiewicz
We had a case the other day which showed poor nuclear detail. This was fatty breast which had been fixed overnight before processing. Tissues were reprocessed in hopes of improving, but failed to do so. Does anyone have any ideas what may have caused this artifact. All other cases that day were fin

[Histonet] shipping slides, elementary question

2010-01-08 Thread Nicole Collette
Happy Friday everyone, I have a very basic question about shipping slides (mouse tissue, non-biohaz). I am planning to ship a bunch to a collaborator, on the order of a few hundred. I have black hinged cardboard/wood 100-slide boxes, similar to https://www.vwrsp.com/catalog/product/index.c

[Histonet] Reichert-Jung 2050 service

2010-01-08 Thread Michael J. Lyon, Ph.D.
We have a Reichert-Jung 2050 that needs service. Any suggestions welcome. Michael J. Lyon, Ph.D. Otolaryngology Research Lab SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice 315-464-7253 Fax315-464-5572 __

Re: [Histonet] pH of 10% NBF

2010-01-08 Thread Geoff McAuliffe
Your pH meter, unless it is a very sensitive (expensive) meter with a very good electrode and has been properly calibrated that day is probably off at least 0.05 - 0.1 pH units so don't worry. Geoff Greg Dobbin wrote: Hi Folks, If the pH of our 10% Neutral Buffered Formalin is reading 7.01 (r