Hi,
Regarding mitochondrial IHC SDHB works well in paraffin sections. I used
the one from AbCam.
Amos
Date: Fri, 15 Jan 2010 19:47:31 +
From: "Hobbs, Carl"
Subject: [Histonet] Re: Mitochodrial IHC marker in FFPE skeletal
muscle
To: "histonet@lists.utsouthwestern.edu"
Mess
Cheryl,
You can set the length of time the slide rack remains over the stating dish to
drain before moving on, also I believe there is also a shake function to aid in
preventing carryover.
I've also noticed on my XL that the eosin evaporates faster that any of the
other dishes. We just top o
when eosin evaporates, the dye concentration increases and adding more eosin
will increase your stain strength. Top with a good quality (not recycled)
alcohol to maintain the level.
This is assuming the level drops due to evaporation, not carry-over.
Cheryl
