Hi dear histology experts,
I will greatly appreciate if somebody can let me know the estimated number of
slides (with 3 sections per slide) per day on average a lab technician can cut
(of frozen tissue).
I am a lab technician ( not a histotech) and work alone in a highly complex
testing speci
Raj,
If you answer Eric's email I may help you too, because I set up a highly
complex testing lab at dermatology starting from scratch with an empty
room when they were starting the remodeling the room. The room may have
already been equipped with ventilation, plumbing and
electrical but they
Hello, All,
I am working on doing some IF stains with bone samples (lucky me!). I
am having a difficult time sometimes to assess the antibody since the
autofluorescence gets in the way. I am using undecalcified, FFPE
sections (late embryo and neonate mouse bones). Without treatment, I
see aut
-Original Message-
From: Chad Miller [mailto:cmil...@labmd.org]
Sent: Wednesday, February 03, 2010 4:46 PM
To: Delaney, Sondra L
Cc: Chad Miller
Subject: FFPE Microwave processed biopsies
Microtomy Issue:
Our lab has recently switched from using GTF (Glycol Tissue Fixative) as a
prim
Place the slides in every other slot of basket and dry for 15-20 min at 69
celcius, let cool in front of fan for 1-2 min. You are able to combine baskets
at this step.
Kyle HT BS
Nacogdoches Memorial
Our lab just received a slide drying oven, and we are trying to figure
out what's a good
We have used it on FFPE EDTA decaled mouse bone, with a little bit of fiddling
with the protocol it works pretty good
Liz
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sherwood, Margaret
Sent: Wed 2/3/2010 3:10 PM
To: histonet@lists.utsouthweste
Has anybody used the TRAP assay kit from Sigma for bone? We want to use it on
mouse tibia for IHC.
If so, 1) can you do it on paraffin and/or frozen sections?
2) if you do it on frozens, do you decalcify first?
3) what type of blade do you use for sectioning on the cryostat?
Our routine sections, picked up on regular slides with no adhesive in the
water bath, dry at 65C for 15 minutes in the oven on our Leica stainer XL, we
never lose sections. Sections for IHC and specials go on Plus slides and dry in
an oven also at 65C for at least 1 hour. Then they get the sa
Thank you everyone for all the information about microarray equipment. As usual
on the Histonet, everyone was quick with the information, generous with their
time, and informative.
Michael Titford
USA Pathology
Mobile AL USA
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It doesn't matter what machine you run them on or if you do them by hand. You
cannot bill for anything that is RUO.ASR and IVD's muct be properly
validated before you bill for them as well.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of S
Another question for the group of experts. Is it true that you cannot
bill for antibodies that are classified as a RUO for Ventana?
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinoi
Where do you get the beads from?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Joyce
Cline
Sent: Wednesday, February 03, 2010 2:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin leac
Humidity has played a large part in our slides leaching Eosin. We have a
dehumidifier in our staining room and we also use H2Blue Beads in our Xylene
substitute. The beads absorb water from the substitute and help prevent
leaching of the stain.
The beads are ordered from Amercian Mastertech.
J
What kind of stainer do you have?
Jan,
Omaha
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anderson, David
W
Sent: Wednesday, February 03, 2010 12:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Hi
We use an IVD p63 (cat# CM163) from BioCare Medical. Contact me if
you'd like further info.
Sally Ann Drew, MT(ASCP)
IHC/ISH Clinical & Research Laboratory
UWHC
600 Highland Ave. DB1-223, Mail Code 3224
Madison, WI 53792
(608)265-6596
Fax:(608)262-7174
-Original Message-
From: histonet-b
>From Cell Marque
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Wednesday, February 03, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] P63
Do any of you
Do any of you Ventana users have a P63 that is not an RUO? If so, where
do you get it from? Thanks a bunch.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
We are having an issue with our GI Biopsy H&E Stains. On our GI Biopsies, we
currently cut eight slides, staining levels 2, 5 and 8 with H&E. The
pathologist are noticing that the level two slide looks great but then levels 5
and 8 will have what they call "Blue Blobs" on the tissue sections.
Just remember that some thermal labels will fade over time. Be sure your
label will still be legible in 10 years.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.
The key thing to note with slide labels is that it's not JUST the label.
It's the combination of the label, ribbon, and printer.
As an LIS vendor, I've heard very, very good things about General Data, and
I do know that GD will sell you the label, ribbon, printer and even the bar
code scanner.
If
We use the labels from General Data through Dako and are happy with the
printing and the resistance to solutions. They adhere well and go through all
types of staining from H&E to IHC and are placed in the slide dryer. We have
treid both the flapless labels and those with the protective "flaps
You can contact Belair in NJ at 800-783-9424
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of S R
Sent: Wednesday, February 03, 2010 10:54 AM
To: histo net
Subject: [Histonet] Microtome PM
Hello Everyone.
Scott,
Did you mount from water or a clearing agent?
Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwest
Glass is generally better for photomicrotomy also.
Pam Marcum
UAMS
- Original Message -
From: "Cynthia Pyse"
To: "Cathy Crumpton" ,
histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 3, 2010 8:21:15 AM GMT -06:00 US/Canada Central
Subject: RE: [Histonet] Tape/Fil
Sammy,
Try Michael Dietrich at Southeast Pathology Instrument Services, Inc at
843-588-2559. I don't know how far north he is willing to go but he is the
best in the business! You can tell him Wanda said so!!!
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
Hello Everyone.
Does anyone know if there is anyone in the northeast that does microtome pm's?
The microtome is a Microm Hm 315. I do not think it has ever had a pm and it
is starting to act up. Thanks in Advanced.
Sammy
___
Does anyone have experience with printed plastic labels for microscope slides
that are claimed to be resistant to various solvents and chemical used in
histolology? If so what product do you use? One company (General Data)
apparently has a special version of their label that is compatible with
Cathy
I prefer the glass coverslips. It seems to give more protection to the
tissue. Recoverslipping is also easier than with the tape. Just my opinion.
Hope this helps.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
Fro
Your xylene and/or alcohols may need changing.
Janet
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Scott Hendricksen
Sent: Wed 2/3/2010 9:02 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
> Hi agai
> Hi again,
>
> Does anyone have an issue with eosin leaching out of sections a few days
> after they have been coverslipped on an H&E stained slide?
>
> Thanks,
>
> Scott Hendricksen HT(ASCP)
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Hi again,
Does anyone have an issue with eosin leaching out of sections a few days after
they have been coverslipped on an H&E stained slide?
Thanks,
Scott Hendricksen HT(ASCP)
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http://lists.
Hello Lynn
I work in a veterinary institution and use a Benchmark. Our vimentin
works very well. Our protocol is stdCC1,16m.
Good luck!
Kathy
Kathleen Jones
Research Technician
Pathology/Microbiology
AVC - UPEI
(902)566-0595
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Histonet mailing li
I cannot help you with your specific enquiry, re the microwave processor,
but gently suggest you learn from this experience and keep a comprehensive
record of methods/techniques used in your laboratory, good luck
anyway
-Original Message-
From: histonet-boun...@l
You did not specify which enzyme you were using, but I am assuming
proteinase K. If an hour's retrieval is too much, and no retrieval is too
little, I would suggest running some (alcohol fixed) controls at 15, 30, and
45 minutes in the 95C, or 1,2,3, and 4 minutes in the enzyme. Evaluate the
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