RE: [Histonet] QIHC

Thank you to everyone for all of your help. I appreciate it very much. Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edu -Original Message- From: Montina Van Meter [mail

RE: [Histonet] QIHC

Here is Ethel's contact information: Ethel R. Macrea, HT(ASCP)QIHC Ventana Medical Systems HC 3 Box 1023, 22191 S. Branch Road Tucson, AZ 85739 UNITED STATES Phone: (520)219-8471 Fax: (502)219-8454 Email: dawnb...@earthlink.net Montina J. Van Meter, HT (ASCP) Lab Manager

RE: [Histonet] QIHC

Pat, The link below will take you to the Self-Assessment Books by the NSH. #6 covers Immunohistochemistry. It is $15 for NSH members and $35 for non members. You will also find other resources available at the NSH website. Histotechnology: A Self Instructional Text, 3rd Edition (Carson and

RE: [Histonet] QIHC

I tried to email Ethel at this email address and was told that it was not a working email address and was "undeliverable". Also, what NSH study guide did you use? Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora

Re: [Histonet] inform HPV III Family 16 Probe (B)

The inform HPV16 is a DNP labeled probe. You should use the ISH Iview plus detection kit for staining. Mark Tarango HT(ASCP)QIHC On Wed, Feb 17, 2010 at 10:35 AM, Marian Powers wrote: > Hi: Any one out there running HPV 16 insitu on the Benchmark XT. I need > some help with which detection ki

[Histonet] Re: IHC Question

Dear Ellen, You wrote: "I'm interested in performing IHC on a mouse ligament.Given their minuscule size, in lieu of paraffin or cryostat embedding and sectioning, we would like to embed in plastic and section at 1 micrometer. Things we are interested in detecting include collagen type 1

[Histonet] inform HPV III Family 16 Probe (B)

Hi: Any one out there running HPV 16 insitu on the Benchmark XT. I need some help with which detection kit to use? Thanks, -- Marian L. Powers, HT(ASCP) Manager, Technical Operations Doctors Pathology Services 1253 College Park Drive Dover, DE 19904 302-677-

Re: [Histonet] Smooth Muscle Stain

Thanks to all who responded again! We thought Trichrome (Masson)- have used it in the past, but, as you know, there is no "specific" stain just for muscle. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit S

[Histonet] IHC Question

Hi All I’m interested in performing IHC on a mouse ligament.Given their minuscule size, in lieu of paraffin or cryostat embedding and sectioning, we would like to embed in plastic and section at 1 micrometer. Things we are interested in detecting include collagen type 1 and III, CD31, Arg

Re: [Histonet] Special stain for Smooth Muscle Cells

Milligan's Trichrome is reputed to be excellent. I have not used it but colleagues have. Milligan, M. "A trichrome stain for formalin fixed tissue". Am J. Clin. Path., Technical Section 10:184-185, 1946. If you have a copy of Humason's /Animal Tissue Techniques/ the method is there. Geoff She

Re: [Histonet] Special stain for Smooth Muscle Cells

Neubert's (1940) is recommended, but a good Masson's trichrome, although not specific for smooth muscle, will show them. It will up to you to recognize the smooth fibers for their location in the subject tissue, and their microscopic characteristics. Uterus will be a good comparison control. Ren

Re: [Histonet] Special stain for Smooth Muscle Cells

Thanks to all who responded, but I was not specific. I am sorry for the confusion. What special stain would you use (i.e. Masson Trichrome, PTAH, etc.) (not IHC) Thanks, again. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General

Re: [Histonet] Stain for Smooth Muscle Cells

Smooth Muscle Actin; Dako; cat. # M0851, clone 1A4 Requires HIER antigen retrieval (I use a Biocare Decloaking Chamber with Dako Target Retrieval Solution) Strong staining on sheep tissue Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnes

[Histonet] Mississippi Society for Histotechnology

Reminder of the upcoming Mississippi Society for Histotechnology Homecoming Event Dates: March 12-14, 2010 Place: Beau Rivage Hotel & Casino, Biloxi, MS Contact: Jerry Santiago at jsanti...@bellsouth.net To register for the meeting, go to the NSH website, then state meetings. Rooms are going qu

Re: [Histonet] Stain for Smooth Muscle Cells

Hi all, What stain do most people use to demonstrate smooth muscle cells? I know there are various choicesWe are looking at sheep lung. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street