Whether you are using an automated stainer or hand staining, run a control
slide and review before any patient samples are stained. I also suggest that
only start or endpoint QC is not enough and you should consider incorporating
continuous QC/QA at regular intervals for the stain set-up, to en
We have automatic stainers, so after the stainer is set up a slide with a
micro-array is run. This slide is
checked by the histologists and logged in. The next slides run are our rapid
cases. A log sheet is
prepared and handed to the pathologist with the slides and they are graded for
processi
Hi,
I will appreciate it, if you guys could share your method/procedure for H &
E QC with me. Thanking you all for your usual cooperation.
Adesupo A.
_
Hotmail has tools
Merced,
We run 700 to 800 slides per day plus specials and IHC's. We use to wipe down
but with staff and workload it was impossible to continue. Thanks for the input
and the remember.
Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907
>
I use VWR VistaVision coverslips (for fluorescent imaging) and had noticed
some dust/debris on them that would autofluoresce and give false positives
with some of my stains. The problem disappeared when I got into the habit
wiping all my coverslips with EtOH before mounting...this may be cumber
Emily, yes we have also noticed real dirty glass also. I will be calling
and checking on what has changed.
Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-422-7907
>>> Emily Sours 03/24/2010 3:26 PM >>>
I've noticed Corning cover slips have b
Hello, Our lab does IHC staining in house and have had zero problems with our
Fisher brand cover slips until recently. We've tried both Fisher and Corning
cover slips and what appears to be dust or imperfections in the glass are found
on both brands. This is creating false positives for us, beca
I've noticed Corning cover slips have been really dirty too, but it
hasn't been a problem for us. It just looks bad.
Not that that solved your problem, just thought I'd throw it out there.
Emily S.
Shall we always be content with the ancient tinned salad of the
subsidized novel? Or the tired ice
We follow the same practice as Susan. Our Pathologists would rather have more
than less!!!
Dawn
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 23, 2010 5:08 PM
To: anita dudley
Hey Brandi,
In my experience carry over generally occurred at time of cut-up or embedding
when tiny bit of tissue get stuck between the groove of the forceps, despite
been wiped clean between each specimens, but if you are 100 % sure that carry
over did not occurred at time of cut-up, or embedd
Hi Benchmark Ultra users!
I would like to hear of your experiences with the continuous workflow of
this instrument. Does it really make life easier or even more complicated?
I think of handling one slide every few minutes after the staining is
completed and of the problems, that occur when the
If not already mentioned, wiping your heated forcep off with a kimwipe or paper
towel before and after embedding each tissue helps prevent this problem at
embedding.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Hello all.
We cut 5 levels on each GI block and mount them on one slide. We use
the really small disposable molds. There are the big polyps or multiple
bx in one container In that case we cut three levels . On our prostate
bx we are cutting 3 levels picking up 4 slides of each level. So
If the colour washes out of the stained sections, the dye is not alcian blue,
whatever the label on the bottle might say. Ask for a refund.
One of the criteria for certifying alcian blue for use as a biological stain is
that the soluble dye becomes completely insoluble and cannot be extracted.
Response to Tjapser:
1 - The cells are in the same location on all levels.
2 - We have a forceps warmer, but we burn the tip in the bunsen flame in
between each case, and we hold the forceps in our hand when imbedding in
succession. We do pause to trim and then go back to embedding (we are a
Hi Brandi,
Don't know if I can solve your problem...but here's a few questions.
1)You've determined that the floater (tonsil cells in this case) are in
the block. Did you determine this because you consistently see the same
floater in the same spot, level after level, slide after slide?
2)Do yo
Anita Dudley, Providence Hospital, Mobile Alabama asks:
>>Just wondering what others were doing with colon [biopsies, endometrial
>>biopsies], ECC's. Do you use one slide or cut 2 to 3 slides per block? Lungs
>>and livers too. We are thinking of going to one slide.<<
Do your pathologists have a
You might want to check the holes that the forceps stand in on the
embedding center too, if you have one like that. That seems to be a
forgotten contributor at times.
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4
Come on, guys!
Who's the first to post "Click link at bottom of mail!"? :D
> Please include me at your mailing list. Thank you.
>
> Dima
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The cells are definitely in the blocks. And we are filtering the formalin bath
also. Thanks all!
Brandi
(prev response I received included here just for everyone's info)
I would be more suspicious of the formalin "bath" that the tissues are
sitting in while they await being placed on the pro
Have you tried sodium thiosulphite? That will leave white spots
instead of brown, which is an improvement and easier to cover.
Lesley Weston.
On 23-Mar-10, at 8:59 AM, Patsy Ruegg wrote:
After you stop laughing seriously I need some help here, apparently
I got my
fingers in some silver nit
You are definite that the cells are in the block? If so I would change all
the paraffins out as well, even the paraffin on your embedding center.
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
tigger...@aol.co
Please include me at your mailing list. Thank you.
Dima
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Hello everyone.
Today we have a problem with contamination. The pathologist notes cells
from tonsil specimens here and there on our GI biopsy slides. The cells are in
the block. I'm trying to ascertain the source of the contamination.
The grossing pathologist grossed the tonsils
We basically do the same at Tom's lab, the only exception is we file the
unstained slides with the stained slides. Especially with the FNA biopsies. We
have had to pull the unstained slide to send out for IHC's.
Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealt
The pH of the stain may be too high, it should be 2.5-2.6 so check it
with a pH meter.
If the pH is fine you/your vendor may have a bad batch of dye since
under normal conditions the stain is difficult to wash out.
Make sure the dye is certified by the Biological Stain Commission. If
there is no
Hey Laurie,
Try with home made Alcian Blue Solution : pH 2.5 1g Alcian Blue in 100
ml of 3% Acetic Acid ( check pH adjust accordingly, filter AB
solution before use) also rather than rinsing with large amount of
water, blot dry section this should help alteration of staining, then
rinse
Hello,
Does anybody have a protocol for paraffin-embedding and sectioning embryos
that have been through whole mount in situ hybridization? Thank you.
Andrew
--
Andrew Gillis, Ph.D.
Physiology, Development & Neuroscience
Anatomy Building, Downing Street
Cambridge, CB2 3DY
U.K.
_
I would also like to hear from anyone using Meditech. We are currently in
the process of implementing this new system with a go-live date of Oct. We
have the Leica IP C and were planning on interfacing, but were just told by
Meditech that we wouldn't be able to.
Diane Weishaar, HT (ASCP)
Hist
We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone
marrows. We also do special stains up front on the bone marrows, H Pyloris,
prostates, etc.
We tend to cut a ton of extra slides that we just throw away. I don't like it
but I don't think there's any way around it. P
Hi Everyone,
Lately, we have been having a problem with our Alcian Blue. It is fine
up to the washing step. Once it hits the water, the blue fades to
unacceptable. We have tried reducing the water rinse after the blue to
just a quick rinse in d-H20, and it is still poor. The vendor replaced
the ki
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