[Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

2010-04-01 Thread Andrew Burgeson
It's a turf warSHARKS AND JETS MAN, SHARKS AND JETS!!! West Side Story played out every day over ego and money. Anyone that tells you they are doing what they do solely for patient care is either deluded or lying. It's not a matter of corrupt...it's a matter of "how corrupt!" Why do plastic

Re: [Histonet] Just three months?????

2010-04-01 Thread Joe Nocito
I see that now, wasn't the first time I was wrong today, probably wouldn't be the last either - Original Message - From: "Jeffrey Silverman" To: Sent: Wednesday, March 31, 2010 5:03 PM Subject: [Histonet] Just three months? CAP is now saying no more gross processing of small th

Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

2010-04-01 Thread Joe Nocito
I've seen lab get accredited one month, only to have CLIA come through the next month and rip that lab apart. I have been through CAP inspections that consisted of the inspector looking only at slides while he checked off the checklist to inspectors carrying their own thermometers and testing th

[Histonet] Gene Patents

2010-04-01 Thread Mark Tarango
Does anyone know if the ruling about BRCA1 and BRCA2 affects MTM labs / Cintec's monopoly on the p16 antibody? Thanks, Mark Tarango ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CAP ANP.22760

2010-04-01 Thread Nancy Schmitt
Hi Histonetters- "Are new lots of antibody and detection system reagents tested in parallel with old lots? Note: New lots of primary antibody and detection system reagents must be compared to the previous lot using an appropriate panel of control tissues." Just wondering how others are handlin

RE: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

2010-04-01 Thread Bernice Frederick
How about all of them? Being as it is April 1. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-bou

RE: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

2010-04-01 Thread Liz Chlipala
Thomas Just a question, what would you consider an unnecessary CAP regulation? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, U

[Histonet] Open position

2010-04-01 Thread Clarke, Mary
Wheaton Franciscan Healthcare in Milwaukee, Wisconsin has an opening for a Full Time Histologist. The position is full time and working first shift. All interested parties should apply at mywheaton.org. Terri Clarke Histology/Cytology Supervisor Wheaton Franciscan Laboratories 11020 W. Plank

RE: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

2010-04-01 Thread Thomas Jasper
I'm inclined to agree with you Andrew. Seems to me that CAP has become, unintentionally (I'd like to believe) something of an unsavory, bullying sort of entity. I'm not certain about all the factors involved, but I think a few things have definitely contributed to all of this CAP negativity. Fir

Re: [Histonet] cassette labels erased by processor

2010-04-01 Thread Lori.Disher
We use "Sharpie, Industrial Super Permanent Ink, extra fine point" without any trouble Lori A Disher Fawcett Memorial Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

2010-04-01 Thread Andrew Burgeson
Correction...I meant to include "High school drop-out" in my example. Furthermore, this is NOT a gender based comment...just a real life example of some of the things I have seen in "CAP" or "non-CAP" inspected laboratories. Regards, AB ___ Histonet ma

[Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

2010-04-01 Thread Andrew Burgeson
Sheesh is right, J. CAP is all politics as far as I am concerned. It is all about protecting the careers and paychecks of the general pathology community. I am thouroughly unimpressed with JCAHO, CAP et al. If all you need to legally run a laboratory is to be CLIA inspected, then WHY BOTHER with

Re: [Histonet] Assay for cell death needed

2010-04-01 Thread John Kiernan
Dead cells stain intensely with anionic dyes such as eosin. For nervous tissue there's a simple method using acid fuchsine and toluidine blue. Normal neurons are blue; dead and dying ones are red. See Auer RN et al 1984 Acta Neuropathologica 64: 177-191. Also Biotechnic & Histochemistry 73: 244

Re: RE: [Histonet] mounting media

2010-04-01 Thread John Kiernan
I've used both the cytoseal mountants (with numbers that indicate viscosity) for several years. These poly(methylmethacrylate) media come in very convenient plastic squeezy bottles for application. They are less expensive than entellan, the classic polymethacrylate mountant, which has to be disp

RE: [?? Probable Spam] [Histonet] New CAP grossing guidelines

2010-04-01 Thread Kim . Donadio
I agree Janice. The only thing it looks to me is to now define Grandfathered in. Because it also states "in addition" for that criteria as well. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mahoney,Janice A"

[Histonet] Pathologist needed for NAACLS

2010-04-01 Thread Jennifer MacDonald
The National Accrediting Agency for Clinical Laboratory Sciences is looking for a Pathologist to sit on a committee. The committee is the Review Committee for Accredited Programs (RCAP). The committee meets twice a year, once in person and once via a lengthy teleconference (day). The face-to-

[Histonet] Pig tissue IHC detection

2010-04-01 Thread Reuel Cornelia
I just wanted to know if anybody who are working with Pig bone tissue fix in 10%NBF and decalcified in 14% EDTA, citrate buffer (dako) antigen retrieval pH7.0 and ph 9.0 by steaming for 20 minutes, what is their IHC detection kit for mouse monoclonal and rabbit polyclonal antibodies. I am using

Re: [Histonet] Assay for cell death needed

2010-04-01 Thread Andrea T. Hooper
Use the TUNEL assay, several very easy to use kits available. Roche's Cell Death Detection kit with FITC might be the easiest. --- On Thu, 4/1/10, Alexandra Meinl wrote: From: Alexandra Meinl Subject: [Histonet] Assay for cell death needed To: "Histonet" Date: Thursday, April 1, 2010, 9:00

[Histonet] FW: New CAP grossing guidelines

2010-04-01 Thread Cheri Miller
That would be my guess; I have on file copies of degree and course study for my grossing personnel Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-b

RE: [Histonet] DAKO Her2 antibody

2010-04-01 Thread McMahon, Loralee A
Do you bake your slides? How old are your cut sections that you are testing? We have found that long baking of the sections before staining? Like overnight at 60C causes the staining intensity to decrease. And if the sections are older than a month the staining intensity decreases as well.

RE: [Histonet] mouse perfusion rate

2010-04-01 Thread Merced M Leiker
Someone I believe on Histonet a couple years ago told me the same thing. The "perfusion circuit" is compromised if you have fluid coming out the nose and internal organs swelling up... Merced --On Wednesday, March 31, 2010 4:14 PM -0700 "Andrea T. Hooper" wrote: Very interesting! Coming

[Histonet] Assay for cell death needed

2010-04-01 Thread Alexandra Meinl
Hello Histonetters, We desperately need an assay that detects cell death in human FFPE sections (regardless if its apoptosis or necrosis). Some kind of kit (quick 'n easy) would be really fine! Any suggestions? Many thanks, Alexandra Dr. Alexand