Hello Histo's:
We are having troubles with getting our Cyclin D1 to stain for longer than one
month. I seems to whimp out after a month, so we make up fresh. Any ideas? We
are using a Dako clone. Also we are currently working up Surfactant A with
little luck. Our lung control does not stain a
I have been asked by a friend to post a fulltime job opening in St. Augustine,
Florida. Must be HT or HTL with experience. Great pay and benefits. Very
flexible schedule. Contact Robin Scott at 904-584-1884 or
rsc...@gi-associates.com
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Histonet ma
HISTOLOGY TECHNICIAN
*Looking for a Lab Environment that…*
· Has been named a Best Place to Work two years running
- Places quality first
- Supports your ongoing learning and training
- Supplies you with state-of-the art technology & equipment
- Provides you with an open, a
Hey does anyone know where to find the FDA and/or AAALAC/IACUC re
gulations on animal tissue retention (mainly rodents)? I know at
prev= ious places we have kept the tissue ForEVer (insert apropriate
Sandlot pron= unciation here), but I just need to know what the actual
I'd use tartrazine or picric acid mixed with acetone.
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Fifteen to thirty seconds in saturated (or almost saturated), aqueous
picric acid will do it.
Geoff
Mike Tighe wrote:
I have seen Tissue sections counterstained with a yellow counterstain. I would
like to use an Iron hematoxylin with this counter stain. Can anyone tell me
what this is? Are t
Brine Group is currently recruiting around a day Histotechnologist
position at a hospital-based clinical lab in southeastern Connecticut.
This is a 4-people lab that is growing. Routine histology, special
stains, and IHC.
This position is responsible for:
* Checking identificat
I use 5 dips and get a great counterstain for mucin
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer
Sent: Monday, May 24, 2010 12:48 PM
To: Mike Tighe
Cc:
Subject: Re: [Histonet] Yellow counters
Mike,
There are a number of yellow counterstains commonly used, including picric
acid.
For your purpose, I would recommend the following in order of preference;
Tartrazine (Acid yellow 23, Food yellow 4) CI 19140,
Metanil yellow (Acid yellow 31) CI 13065,
Martius yellow (Acid yellow 24) CI 103
We use tartrazine for a yellow counterstain (on mucin or VVG). It
stains quickly, so usually all you need is a few seconds.
Drew
Sent from my iPhone
On May 24, 2010, at 12:13 PM, "Mike Tighe"
wrote:
I have seen Tissue sections counterstained with a yellow
counterstain. I would like
Sounds like you might want Metanil Yellow. I have found it doesn't fade as
much if you let your slides dry a little before you dehydrate and
coverslip.
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
"Mike Tighe
Metanil yellow...and it's kind of a pain in the rear. Look = up a
stain for goblet cells in intestine and there should be a procedure
fo= r it. I have pulled my hair out using it and not getting an
overcount= erstained slide.
Good Luck!!
Sarah Goebel, B.A., HT (A
I have seen Tissue sections counterstained with a yellow counterstain. I would
like to use an Iron hematoxylin with this counter stain. Can anyone tell me
what this is? Are there any tips/tricks to this counterstain? Thanks in advance!
Mike
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Dear Histonet Members,
I hope you are doing well. I am a Recruiter at a highly successful and well
respected Healthcare recruiting firm. I help place Lab Professionals in
permanent positions across the country and I wanted to see if you are
interested in exploring other career opportunities?
There shouldn't be any problem if you are doing immunoperoxidase staining. If
you are doing fluorescence, you will have to be sure you remove all traces of
eosin from the tissues since eosin itself is fluorescent.
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Hi Histonetters!!
I hope everyone had a great weekend and is looking forward to a fun
filled Memorial Day Holiday weekend.
I have some great job opportunities I want to tell you about All of
these positions are full time permanent positions with excellent pay,
benefits and relocation assistance.
Jennifer,
If you are looking in NY - you will need to get a license through the State
Department of Education. Some NJ jobs will also ask for NY license as
well. Not knowing what your educational background is, it is something I
would get at as soon as possible to see what you may need.
The gen
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