Hello everyone,
We are trying to stain alpha smooth muscle actin (on mouse/human liver) using
the A5228 Sigma antibody and are getting nuclear staining while this stain
should be cytoplasmic. Does anyone have experience with nuclear staining of
alphaSMA? Is there anything I can do to
Histonetters- Region II has extended the mail-in registration deadline,
until Friday May 28th...just a few days away! We know some people are
still awaiting travel approvals and we are doing our best to accomodate
everyone who wants to attend and take advantage of the early
registration. I went to
I am trying to section an otolith from a small minnow species with a
vibrotome. The bone seems to be very dense and doesn't hold very well inside
any mounting media that I have tried. Does anyone have any suggestions as to
a media that will section smoothly but would be dense enough to hold the
sma
Do you want to sell your old Autotechnicon ?
I'll buy any mono or duo Autotechnicon .
> From: aalli...@wpalabs.com
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 26 May 2010 08:56:02 -0700
> Subject: RE: [Histonet] Autotechnicon?
>
>
> My God Tom, I haven't used an Autotechnicon or U
Allison Scott HT(ASCP), Histology Supervisor, LBJ Hospital, Houston, Texas asks:
>>Our [pathologist's assistant] used Higgins ink (india ink) on some skin
>>specimens and set the ink with 10% acetic acid solution. After the specimens
>>were processed, all of the ink was gone, washed off. We have
Hi Histonetters!
I hope everyone is having a great day. I have a few new jobs that I
want to tell you about. These are new positions with some of my best
customers. I have people working onsite that just love thier jobs so if
you are interested in looking into a new opportunity in one of these
a
My God Tom, I haven't used an Autotechnicon or Ultra-Autotechnico= n
since 1979! Was it an Mono or a Duo, or the old black spider? = I'm
sure you know what I mean Tom, 1 or 2 levels. I guess = it's better
than working in China, reusing their stained slides by sca= ping off
Hi Allison, Trying to think why it washed off, I wondered if the skin went back
into formalin after the acetic acid, or did it go to alcohol, perhaps setting
up an acid alcohol condition that maybe was enough to decolorize it. Tom T
-Original Message-
From: histonet-boun...@lists.utsouth
After India ink we always used acetone.
René J.
--- On Tue, 5/25/10, Scott, Allison D wrote:
From: Scott, Allison D
Subject: [Histonet] Higgins Ink
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, May 25, 2010, 4:13 PM
Hello to all in histo land. Our PA used Higgins ink (india ink) on s
MaryAnn Dixon BS, HT (ASCP)cm
Biological Scientist
Surgical Oncology
UF College of Veterinary Medicine
Phone (352) 294-4516
Email: dix...@ufl.edu
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We use Higgins as well. For many years we have set it with nothing more than
regular white vinegar.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original Message-
From: histonet-boun...
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