[Histonet] PGP 9.5 Frozen Tissue

Is anyone out there doing the PGP 9.5 on fresh or frozen tissue? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Laura Miller is Out of the Office.

I will be out of the office starting 07/08/2010 and will not return until 07/09/2010. I am out of the office for the rest of today. I will reply tomorrow, Friday, July 9th. __ This email has been scanned by the MessageLabs E

[Histonet] Helf- strange IF result

Hi, I am detecting Arc (Activity regulated cytoskeletal protein) protein in rat brain slices. I perfuse transcardially the animal with 0.1M PBS and than 4% PFA with 5% sucrose. Post fixation in 30% sucrose 1% PFA. In some cases when i take out the brain after the perfusion i make small cut or inc

[Histonet] Histo bath

I am looking for a condenser cooling fan motor for my Histo -Bath. Thank you for any Help . Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, us

[Histonet] AP Computer Systems

We are currently looking at a overall computer system for our Hospital. My point of this question is has anyone worked with and their feelings about the following vendors for the AP application: Meditech Cerner Quadra Med Confidentiality Notice: This e-mail message, including any attachments, is

RE: [Histonet] Peloris Users

I you are going to choose to override the prompts, you had best revert back to a schedule of changing solutions on a rotating schedule. You probably had something like that in place with your previous processor. I would call Leica to have them send out someone to help get your processing optimiz

[Histonet] PGP 9.5 antibody on Bond stainer

I have been asked about bringing this antibody in house and I was wondering if anyone is staining for this using a Leica Bond stainer. Any advice, tips, etc. would be appreciated. We are looking at getting the antibody from Dako, cat# Z 5116. Thanks in advance for your help. Martha Ward, MT (AS

Re: [Histonet] Peloris Users

The machine will work well only if it is set up well and the staff follows all of the prompts. We set up ours with two stations of 80% alcohol in the beginning to make sure it starts with a good graded series of alcohols. Another thing to look at is the biopsy pad percentage. Setting it right wil

RE: [Histonet] Peloris Users

We have helped that problem by being sure there is adequate graded alcohols on the Peloris. There must be between 60-70%, 80-90%, and 95% before you have the 100% alcohols. If the concentration's are too high there is inadequate infiltration of the tissue's. We usually only use the 2 hour and

[Histonet] Peloris Users

To all you Peloris users, here is my delimma. When we follow the machines prompt to change the solutions because of purity and we then process small biopsies on the machine, it overprocesses the specimens. So the staff have been overrriding the prompt to change the solutions causing specimens t

RE: [Histonet] Reprocess

What Tony suggests is similar to what we do for reprocessing. If we have a block that we embedded and attempted to section, only to discover that it's not fixed well enough, we do the following: 1) print a new processing cassette 2) using a "dull" knife (we have an old, dull steak knife), run the

[Histonet] Vendors & others: X-reactivity between HSV I & HSV II?

Having switched from an HSV I & II antibody cocktail to separate antibodies for HSV I and HSV II, we have found (and the manufacturer has confirmed) that the two separate antibodies cross react. Does anyone know of antibodies against HSV I and HSV II that do not cross react? Thanks, Linda A. S