Rather than use Halt (or other adhesive) in your water bath, have you tried
using charged slides - those used for IHC. Then air-dry or heat dry the
tissues before deparaffinizing. Any water left in the tissue will cause
wash-offs. Do not lower the pH of the silver solution.
Cheryl Crowder,
Histonetters,
It has been so long since we had to order these slide racks, I am can only
hope they are still made/available. Lipshaw(that's digging into the past!)
/ Shandon / ThermoShandon used to carry them but not seeing them on Thermo
Scientific website. What has been found are metal rac
Brian,
What helped me a lot with stains, fixatives, etc, was to make a chart of each
of the stain or fixative "families" (silver, trichromes, etc) and list the
method steps of each, components used, and purpose of the components. That put
in perspective the reasons for the differences, which a
Dear Histonet members,
I have been cryosectioning Xenopus laevis embryos this summer that have
undergone a whole mount chromogenic in situ. The embryos are then fixed
overnight in a formaldehyde solution (1X MEMFA) at 4 C, then stored in 1 x PBS
until they are cryoprotected in a sucrose so
Does anyone have any advice on good study aids, areas of prep to concentrate
on, or any test taking strategies that helped them? I have been pouring over
the 3rd ed of "histotechnology, a self instructional text" for months but since
I have started to look at the ASCP/ NSH discussion boards i
I will be out of the office starting 07/29/2010 and will not return until
08/02/2010.
Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
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htt
Hey, Sara!
Congratulations.
Enjoy all them Hassles and Advantages in your NEW lab.
Best wishes,
Carl
NB: re Formalin fixation...one could argue that one should store such-fixed
specimens in 90% alcohol, after the appropriate Formalin fixation time?
OR...30% Sucrose: now, THAT is a PRESERVATIVE
We 1st use straight acetone and leave the slide in anywhere from 1 to 3
minutes, then 20 seconds in 50/50 acetone/xylene, then dip in straight xylene.
Depending on how long the slide has been coverslipped, the times will need to
be adjusted.
Mighnon Lashus, HT (ASCP)
PathGroup Lab
4071 S. Acce
We use a 50-50 solution of xylene and acetone. Depending on how long the
coverslips have been on, it takes from 20 minutes to an hour for them to come
off. If you are not decolorizing and re-staining, don't leave them too long in
the solution as the stain will start to come out.
Sharon E. Sc
The slips will not come off with xylene
Soak in acetone for 5mins
The slip swells and falls off
Without letting the slide dry or turn milky white, dip into xylene a few times
If milkiness appears, dip in clean acetone a few times and back to xylene
Recoverslip as and when needed
Simple really
Anni
We soak the slides in acetone for 5-10 minutes and the film will peel off
very easily.
Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgo...@clinlab.c
Hello all -
Is there a more effective and faster way to remove the film from the slide?
I had to decolorize a couple of H&E slides to do special stains, and it took
5 days for the film to remove. The slides were only a few days old and I
soaked it in fresh xylene. I almost removed it too soon
Northwest Pathology has an opportunity for an Immunohistochemistry Technician,
ASCP certified or registry eligible preferred, to join our progressive
laboratory. This full-time position requires two to three years of hands on
immunohistochemistry experience with QIHC certification or eligibili
I get my mouse p53 Ab from LabVision...
http://www.labvision.com/ab.cfm?First=AntiBody&Second=9006
--
Message: 18
Date: Thu, 29 Jul 2010 10:56:56 -0600
From: "Ross Benik"
Subject: [Histonet] p53 IHC staining in mouse
To:
Message-ID:
Content-Type: text/plain
Sandy,
We keep a log that says the alcohol was tested for %, date and tech. The
recycled alcohol goes into already labeled containers that notes that it is
recycled and the %. The recycled Formula 83 is tested for purity and entered
into the log the same way the alcohol is.
-Original M
Dear Histonetters,
If you use a recycler for xylene and/or alcohol, how are you labeling
the recycled containers? Do you assign a "lot #" to each carboy?
Do you keep a log or just label any container filled from a carboy with
the "lot #" and the %, in the case of alcohols?
Thanks,
Sa
Yes, ORO stains lipids but I don't think it would work for crude. Would you
look for artifact and tissue reaction?
Pam Vlies, HT-ASCP
Waukegan IL
Sent on the Sprint® Now Network from my BlackBerry®
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
Sender: histonet-boun...
GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde)
solution, Davidson's fixative (3 parts water, 3 parts alcohol, 2 parts
37% formaldehyde, 1 part glacial acetic acid), and various proprietary
products (Dissect-Aid, O-Fix, and others) are all used to fix fatty
tissue in order to
Greetings histonetters!!!
Does anyone out there have a paraffin knife holder (knife holder B) for a Leica
SM2500 polycut microtome that you might like to part with. I am in need of
finding one. Thank you.
MaryAnn Dixon BS, HT (ASCP)cm
Biological Scientist
Surgical Oncology
UF College of Veteri
Hi everyone,
I am trying to accomplish IHC staining for a rabbit anti p53 antibody in
mouse tissue however all I am getting is background and IgG staining. I
run my human positive control tissue along with the same protocol
parameters and the staining is perfect. I have tried two abcam
antib
Hi histoland,
Thinking of getting into plastics and need to know information, pros/cons about
them. I've never worked with anything else except paraffin. I will be
embedding mostly osteosarcomas. Can someone please give me a call or email me.
All suggestions are very appreciated. Thank you.
M
Thanks to everyone that replied to my question about refrigerating 10%
NBF for up to 6 weeks. I'll fold your information into the decision.
Sally Breeden, HT(ASCP)
Veterinary Diagnostic Services
New Mexico Department of Agriculture
700 Camino de Salud NE
Albuquerque, NM 87108
505-841-257
Hi,
I'm using FAST profile (Fast green, Alcian Blue, Safranin-O, and Tartrazine) to
stain my human Intervertebral Discs. how can I figure out which color resembles
which type of tissue?
Regards
-Sarah
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I agree with Rene and Mark.
Geoff
Breeden, Sara wrote:
We're getting ready to move into our new building (YAHOO!) in early
September - finally. In planning where we will store our cases for the
required 6-week retention time, I have proposed that the tissues (in 10%
NBF) be shelved in the walk
Good Morning All,
Our team at Personify is currently partnered with a World Leader in IHC that
is currently looking for a Field Support Specialist in the New England area.
This is the perfect opportunity for a histotech to move off the bench and
get into the field on the manufacturers side!
I had a retic kit that was put in the refrigerator by one of my
collegues. The formalin was stated to be stored at room temp, but was
left in the box. The cold temperature broke the formalin down and it no
longer worked to reduce the silver. My suggestion would be to keep them
at room temperatur
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