This is how professional histotechs treat each other? A very sad commentary in
a country where people are afforded free speech.
Maria Katleba MS HT(ASCP)
Pathology Dept. Mgr
Queen of the Valley Medical Center
1000 Trancas Street
Napa CA 94558
(707) 252-4411 x3689 direct
(707) 226-4385 pager
(707)
I thought Histotechs were supposed to have purple thumbs. :)
(You know, gardeners have green thumbs...)
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of O'Donnell, Bill
Sent: Tue 9/7/2010 4:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [
I know... I should wear gloves when doing a GMS.I... know... that.
(sorry, I thought this was Facebook for a second) Have a great week!
- Sir Bill of the Blackened Thumb
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Histonet mailing list
Histonet@lists.utsouthwestern.edu
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Gee... It's not like WE have work to do or anything. Thanks for the heads up
about these companies.
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of
kim.dona...@bhcpns.org
Sent: Sat 9/4/2010 10:55 AM
To: histot...@imagesbyhopper.com
Cc: histo
We are experiencing some problems with our trephine bone marrow biopsies.
Could anyone share with me the amount of time they are generally using for
decalcification and what reagents you are using? We are also experiencing
fragmentation in some of the slides. Thanks
James Vickroy BS, HT(AS
Hi,
I am processing rat infraspinatus tendons for Masson Trichrome staining. I've
had trouble with routine processing protocols and read that adding 4% phenol to
the 95% alcohol solutions may help soften the tissue to make it easier to cut.
Has anyone ever tried this, or have other suggestions
Hello,
Does anyone know of a good control tissue absent of (or with few) macrophages?
I have seen a number of postings regarding tissue positive for various
inflammatory markers (F4/80, MOMA. CD68).
I am running F4/80 (Serotec, Rat anti-ms F4/80) on ms. tissue. I have a number
of positive
Hello Everyone!
I have searched the archives because I am sure this is a problem that has
been encountered before, but I have been unsuccessful in finding the right
thread. I ran an IHC series dilution on a new primary antibody for NeuN. I
am using 35 um rat brain sections that were cut on a cryos
Formalin fixes slowly, even more slowly at 4 degrees C. Even though the
fix may penetrate, fixation will not be complete.
I suggest a formalin+alcohol+acetic acid fix at room temp for 24 hours.
There are many, many variations of this fix. Lillie recommends 85 ml of
95% EtOH, 10 ml conc. formalin
Thank's all,
I'm thinking that my IHC method might be O.K because it is working great
with other paraffin embeddedd tissues like diaphragm and various muscles,
and (sometimes) also in older liver tissues that i have found in our lab
(but i don't know how they were made).
I'm using antigen retriev
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