[Histonet] HMGB and RAGE; TLR2, TLR4 antibodies

2010-09-29 Thread Fabrice GANKAM
Hey guys Just wanted to know if any of you had some luck with an antibody aigainst rat HMGB1 and its receptors RAGE, TLR2, TLR4. W Thanks Dr Fabrice GANKAM UTSW ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.e

[Histonet] (no subject)

2010-09-29 Thread Sean McBride
Hello everyone, I have an old Leica RM 2065 microtome that is in need of repair (the clutch stopped working), but according to Leica, the machine is no longer supported by the company. Does anyone have any suggestions for a company or technician who might be able to repair the machine? Thanks in

[Histonet] RE: Bag Sealing system

2010-09-29 Thread Anthony Reilly
Hi Loralee Try a butcher's supply store. They sell devices they use for vacuum packing meat. By removing the air from the bag the evaporation of formalin is almost zero and the specimens will remain intact for many years. The bags are also more hardy than those sold with basic bag sealers.

Re: [Histonet] Bag Sealing system

2010-09-29 Thread Joseph Saby
Perhaps the best and least expensive tissue save bag sealer I have found can be purchased from ULINE.  There are benchtop models that easily fit in a hood, there are floor models.  The benchtop model we use cost less than $200 USD, easily fits in a hood, and has been totally trouble free for 2 y

[Histonet] Free floating sections

2010-09-29 Thread Alexia Francisca Núñez Parra
Hello Histonetters! I am currently running a immunofluorescence reaction using free floating brain sections and am having problems with the sections folding as I lift them to mount at the end of the reaction. To obtain the sections, I first perfused the mouse with 4%PFA, dissected the brain

[Histonet] Ventana Ultraview DAB detection kits for sale.

2010-09-29 Thread Tim Higgins
Hello Histonetters, We are overstocked on Ultraview DAB detection kits and looking to sale some for $2,300.00 per kit. We will pay for shipping within the continental US. The kits are NOT registered with the button still attached. We have 6 with the expiration Date: 4/2011 Lot #812955 We

[Histonet] Used Microwave processor for sale

2010-09-29 Thread Sharon . Davis-Devine
We have a used Milestone RHS I Microwave Rapid Histoprocessor that we don't use anymore. We will be remodeling the histology lab in the near future and don't have room for it, so anyone out there in Histoland interested in this piece of equipment? It is in excellent shape and we have lots of e

Re: [Histonet] histomorphometry

2010-09-29 Thread Silvina Molinuevo
Hi louise! like Yak-Nam WangI do use Image J. It is very easy to use, you can use just a picture and count particles/cells manually or do it  automated after defining the size threshold. The last way It will take you a bit more time until you validate the process, but it is no difficult. cheers,

[Histonet] The Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Morphology

2010-09-29 Thread Carrie Disbrow
HI! I was hoping to find out if I would be able to apply CEU's from the Feb 2011 Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Morphology to the State of Florida Department of Licensing. Does anyone know? There is not a lot of time to get the discounted rate f

RE: [Histonet] Bag Sealing system

2010-09-29 Thread WILLIAM DESALVO
Cardinal sells the Kapac sealer and a variety of sizes of bags at 2 mm & 4 mm bags. This system is industrial and intended for laboratory use. We use to store tissue on-site and transport tissue and cassette between sites. has reduced storage space and incidence of fluid spills. William DeSalv

RE: [Histonet] Bag Sealing system

2010-09-29 Thread sgoebel
I have been using a food sealer from Walmart (or the likes) for y ears. You can buy the food seal bags in precut sizes or you can buy a= roll and make your own. The sealer is around $100 (or less) an= d the bags vary but are WAY less expansive then buying from a scientific

[Histonet] RE: Bag Sealing system

2010-09-29 Thread McMahon, Loralee A
Milestone Medical has a really neat system. www.milesonemed.com It is called the TissueSAFE. You will find it under preanalytical tools. We would love to get one for our couriers and biospecimen repository. We haven't purchased it yet. Loralee McMahon, HTL (ASCP) Immunohistochemistry Sup

[Histonet] Bag Sealing system

2010-09-29 Thread Masterson_John
Hello, Can anyone recommend a bag and heat sealing system for archiving/shipping tissue? Thanks in advance. John This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received t

Re: [Histonet] Problems with listserv

2010-09-29 Thread V. Neubert
YMMD! You are the first I notice who wants to subscribe, not unscibe or unscribe or unsubscribe :D http://lists.utsouthwestern.edu/mailman/listinfo/histonet > I am having some issues with receiving email from the listserv. I was > successfully receiving email and it suddenly stopped. If I ha

Re: [Histonet] Problems with listserv

2010-09-29 Thread Mark Tarango
* *I think the chatter just died down since many of us are at the NSH symposium. Mark On Wed, Sep 29, 2010 at 8:49 AM, Feher, Stephen wrote: > I am having some issues with receiving email from the listserv. I was > successfully receiving email and it suddenly stopped. If I have been > unsubsc

[Histonet] Problems with listserv

2010-09-29 Thread Feher, Stephen
I am having some issues with receiving email from the listserv. I was successfully receiving email and it suddenly stopped. If I have been unsubscribed, please re subscribe my email address. sfe...@cmc-nh.org Thank you, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Cathol

[Histonet] FW: DAB

2010-09-29 Thread Cynthia Pyse
From: Cynthia Pyse [mailto:cp...@x-celllab.com] Sent: Tuesday, September 28, 2010 3:48 PM To: 'histonet-requ...@lists.utsouthwestern.edu' Subject: DAB Hello Histonetters I need some expert advice concerning the disposal of DAB. Our lab is in NYS and there has been some discussion on the

Re: [Histonet] Vectastain elite ABC kit- multiple dips ok?

2010-09-29 Thread Geoff McAuliffe
Teresa Iglesias wrote: Hi all, I have three 24-well plates with three brain sections in each well. For IHC, do I have to prepare three different/fresh ABC dips for each 24-well screen plate or can I incubate (30min) each successive screen plate into the already used dip of ABC? I would not. Do

[Histonet] (no subject)

2010-09-29 Thread lksell
http://infioratadigerano.org/100298.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet