There are now online CAP PIP programs. I have done one on prostate.
Also there are online programs for the cytology part. Review the
catalogue.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
bill.t
The following might be of use
Marking surgical margins are often required for determining the adequacy
of excision of lesions. Sometimes up to 6 margins (medial, lateral,
superior, inferior, superial and deep margins) are required to be
individually marked.
The properties of a good marker include
From: "Laurie Colbert"
Subject: [Histonet] PI for Pathologists
Laurie Colbert at Huntington Hospital in Pasadena CA asks:
>>Our pathologists do not like the CAP survey for their performance improvement
>>program in Surgical Pathology. Does anyone know of any other
>>company/organization that m
Hey All,
I met with a guy today who mentioned he was looking for 3 or 4 techs for
a histology lab he is opening. I told him I would post the opening on
histonet. His information is:
Dr. Nabil Hatoum
Experimur Toxicology
4045 S Morgan St
Chicago, IL 60609
773-254-2700
www.experimur.com
Good l
Neil,
You need to do some fixation. Hopefully they are mounted on charged
slides.
We routinely post-fix fresh-frozen human brain sections in cold
Acetone/ETOH (3:1) for 10 min and then proceed with the IHC staining.
Brett
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
1- they are very delicate, so treat them accordingly!
2- you do not need to use antigen retrieval, since they have been sectioned
frozen.
3- go directly to block→Ab→detection→chromogen→ H&E counterstain.
4- the times could be shorter than with FFPE sections.
5- wash very delicately.
6- dehydrate a
I have limited experience working with human brain tissue and would like to
stain some human brain sections that I received. They were not fixed, but were
flash frozen and sectioned on a cryostat, and mounted onto slides.
Could someone give me a brief run down of their standard methodological stai
I used to mark on the slide, around the section, with a wax marker. Once dried
and the procedure completed, cover and observe.
René J.
--- On Mon, 10/4/10, ba...@gundluth.org wrote:
From: ba...@gundluth.org
Subject: [Histonet] Direct immunofluoresent question
To: histonet@lists.utsouthwestern
Is Michel Medium the same as Zeus? Do these need to be refrigerated?
Thanks.
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Hi all -
The pathologist that reads our direct IF slides likes to have the sections
of tissue circled on the slide so that they are easier to find. We
currently use black marker on the back of the slide, but find that many
times it smears and are looking for a better solution.
What kind of slid
Our pathologists do not like the CAP survey for their performance
improvement program in Surgical Pathology. Does anyone know of any
other company/organization that might offer something like this for our
pathologists?
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`HT or HTL ASCP-tech will perform duties in a veterinary histology lab
at Medimmune in Gaithersburg Md. Tech should be able to perform
full-tissue necropsies on rodents, subsequent trimming, perform manual
and automated tissue processing, decals, embedding in OCT and paraffin,
microtomy and cryoto
thanks Amos
which one did you used ? you have catalog number ?
On Fri, Oct 1, 2010 at 9:44 PM, Amos Brooks wrote:
> Hi,
> I am currently working on HMGB1. It is a weird one! If you go by the
> data sheets you will get great nuclear staining on all the nuclei. This is
> not what you are afte
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