Yes, soak slides in xylene to remove coverslip.
A few days in 37 degree oven won't hurt
Roxanne
Sent from my Verizon Wireless Phone
- Reply message -
From: "Lewis, Patrick"
Date: Thu, Oct 14, 2010 7:11 pm
Subject: [Histonet] I have a couple of quick questions
To:
If I want to remov
If I want to remove a cover slip that is coversliped with permount. Do
I need to use xylene to get rid of the semi hardened permount gunk?
Also, If I am heating paraffin sectioned slides overnight at 37C to
adhere them what happens if I over cook them by leaving them at 37C for
more than one
The date is set, the topics are in place, and the money is just right, all
we needis you to attend.
When: Friday, November 5, 2010
Where: Somerset Medical Center
110 Rehill Ave
Somerville, NJ 08876
"2010 Focus on IHC" presented by NJSH and sponsored by BioCare Medical, LLC
dear all
i'm going to detect alkaline phsphatase in avian intestine and i didn't do that
befor so any help with protocol?? or kits for that and how to use it
can we detect it in FFPT?
by the way i have sent a question twice befor about how to diff. between red
muscle and white one and have no
Histonetters,
Does anyone know if Zinc Fixative(J B Fixative) inactivates viruses and
prions? I need a reference and haven't found one in numerous literature
searches. Our lab is concerned that there may still be active virus in
zinc fixed tissue since it doesn't contain formalin.
Thanks,
Dear Histo-netters,
I am trying to work up a phosphor-akt antibody (Cell Signaling #3787)
for my FFPE blocks of mouse uterine tubules. Fixation is in Z-fix for
48 hours. So far I have tried HIER with both microwave retrieval and a
pressure-cooker. I am using DAKO's En-vision plus rabbit second
*Molecular & FISH technologist*
* *
*SUMMARY*
Under the supervision of the Histology Supervisor the Molecular & FISH
Technologist performs the tests and procedures assigned to the Molecular
Pathology Department. The position is responsible for specimen
accessioning, preservation, processing, te
Is anyone aware of regulations stating that formalin has to be locked up- put
in locked cabinets when not under direct supervision?
Applying to either clinics where specimens are collected into formalin
containers or in the pathology lab?
Thank you
Victoria Spoon
Anatomic Pathology Manager
B
Hello,
I am just sending this email out because I am working on a couple
Dermatopathologist job openings. Please let me know if anyone out there is
interested or knows someone interested (we offer referral bonuses) in a
Dermatopathologist poistion in Atlanta, GA area or in the Milwaukee, WI
area.
Thank you so much for the source information!
Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
Chicago, IL
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
CMS/NCCI Update Dated October 1, 2009
8. The unit of service for special stains (CPT codes 88312-88313) and
immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If
it is medically reasonable and necessary to perform the same stain on
more than one specimen or more than one block o
Try the web site of the vender that you get your antibodies from.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr
Sent: Thursday, October 14, 2010 11:41 AM
To: histonet@lists.utsouthwestern.edu
Hi Jo-Ann
We are trying to sell a VIP 3000 right now. It is closed and has really cool
magnets to operate it. It is in good shape and a real work horse. It holds 300
blocks and is a benchtop model.
Please let me know if you are interested.
Thanks
Stacey Langenberg
CU Dermpath
Sent via BlackBerr
The change in place now allows for billing multiple blocks from the same
specimen for the same special stain (it isn't just immunos) BUT you
must justify this (should be done by a statement in the report micro).
You are not permitted just because you wanted to stain multiple blocks
to increase you
Try IHCWORLD Image Gallery.
http://www.ihcworld.com/imagegallery/
Jan Shivers
UMN VDL
- Original Message -
From: "Michele Carr"
To:
Sent: Thursday, October 14, 2010 10:40 AM
Subject: [Histonet] Immunohistochemistry images
Hi everyone, I was wondering if anyone knew of a website th
Can anyone give a reference for this change in IHC billing. I'd like to have it
in writing when I suggest the change for our lab.
Thank you.
Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
Chicago, IL
*** Confident
Hi everyone, I was wondering if anyone knew of a website that I can view and
save the IHC images. We are putting together a new procedure manual and the
pathogists want images of the antibodies we use to be included in the manual.
Thanks in advance for your responses.
Michele Carr HTL ASCP
Medi
Can you site your source, please.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Thursday, October 14, 2010 10:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Hist
We are looking for a new, smaller histo-processor. We would like a closed
system, I really don't want one of those small round things that has to be
kept under a hood. Anyone out there has a recommendation for a table top
model. It is for student use so there would, probably never be more
The change is that you can bill per block now and not per specimen. This is for
immunos and special stains. It does make a huge difference!
Best,
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - F
1% hydrochloric acid will eliminate the hematoxylin. You do not need to repeat
HIER (if the section has already gone throught it).
René J.
--- On Wed, 10/13/10, Margaryan, Naira wrote:
From: Margaryan, Naira
Subject: [Histonet] FW: How to remove Hematoxilin
To: "histonet@lists.utsouthwestern.
If you don't want to pay $2000 for software, you should just make your own
excel worksheet. Have one person be in charge of it and no one is allowed
to put anything in the freezer without asking for permission. I had to
clean a departmental freezer once, and it was awful. So much stuff no one
kn
Phyllis,
We hold all our needle cores overnight and pre-process for 15 min. The problems
we are having is shrunken nuclei and nucleoli are not distinct even in
carcinoma cases. Cracking artifacts of cores (longitudinal), darker and paler
staining along the length of the cores. We tried Harris bu
Hi Richard,
We are a research facility and have the same issue with not always knowing
where and whose stuff is in the freezer or refrigerators. Even with the best
system though you still have to have people who communicate that they are
taking stuff out and also replacing back where it was rem
Here here Amos. This is why I am for using multi tissue controls that have
considered a range for most of the differences in tissues, fixation and
processing encountered.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihct
Does anyone out there have an effective policy for monitoring the
storage of material at 4C,-20C or -80C. At the moment if any more space
is required the solution is usually to buy another fridge or freezer,
which then takes up more floor space, uses more electricity
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