There are so many good to great processors on the market, but all have their
plus and minus issues. You really have to decide what your two or three most
important issues will be and then rank them. With the trend in becoming more
efficient/cost effective, reducing TAT and LEAN process improvem
We use an aqueous mounting media from Diagnostic Biosystems. Available in two
sizes. Same as the aqua mount
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histon
Is Aqua-Mount aqueous mounting medium still available? If so, who sells
it? If not, recommendations for a similar product?
Thanks
Paul
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How are you meeting the hours of fixation requirement for Breast? With
2 and 4 and 8 hours,, But recently there are articles calling for Her 2
to be done on GI cases. Just want to know you insight on this?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histone
We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol. We run 2
hour protocols throughout the day with an average of 4-5 runs per day
depending on specimen volume. We really like this processor. We have
had them for 10 months now, are using factory protocols and have not had
any specimens
For the specimens you mention use EDTA pH7
René J.
--- On Fri, 10/22/10, Lewis, Patrick wrote:
From: Lewis, Patrick
Subject: [Histonet] Recommendations for decal solution
To: Histonet@lists.utsouthwestern.edu
Date: Friday, October 22, 2010, 4:06 PM
Hi guys,
Can anyone recommend a good dec
Best decal in the world...Formical-4. It has formalin in it so it fixes
and decals at the same time. You can get plain Jane decal too. The
company is called decal. Here is the website. I think you can also get
it from Fisher?
http://www.decal-bone.com/
Sarah Goebel, B.A., HT (ASCP)
Histot
Dear Histonetters
Yes I should have said, I'll be doing IHC for viral proteins with both
polyclonal (rabbit) and monoclonal (Rat/Mouse) primary antibodies and
HRP-labeled secondary antibodies with DAKO AEC Substrate.
Patrick Lewis
Research Associate II-Bench| Infections and Prematurity
We don't, Leica recommends that you don't. We have our grossing group
squirt a 0.1% Basic Fuchsin on the small translucent or white specimens. It
leaves it light pink like the eosin.
On Fri, Oct 22, 2010 at 11:09 AM, Rathborne, Toni <
trathbo...@somerset-healthcare.com> wrote:
> Does anyone use
We are in need of some advice regarding rapid tissue processors. Models we
are considering:
Sakura Xpress
Leica Peloris
Thermo STP 420
It seems none of these models are perfect in every respect. I'm interested
in anyone's opinions of these processors and your experience with them.
All input is
I am one of the founders of a healthcare recruiting firm. I help Lab
Professionals find permanent employment and I wanted to see if you or anyone
you know is interested in exploring other career opportunities? We are
completely free of charge to candidates and and we work on laboratory openin
I like 5 to 10% formic acid
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-Original Message
Hi guys,
Can anyone recommend a good decal solution. I have some bone marrow and
trachea tissues for paraffin sectioning and I want to decal them.
Thanks
Patrick
Patrick Lewis
Research Associate II-Bench| Infections and Prematurity
Seattle Children's Research Institute
206-884-111
Our LIS specialists built a template into the pathologist screen for
each case where they simply check off the boxes of the IHC they want on
a case. It took them a little while to get used to not using post it
notes for their orders but once they got used to it, they like it. It
only takes them a
No, once you have removed the coverslip, just wash the section thoroughly in
xylene to remove any thick remnant of the old permount, and reapply permount
and cover.
René J.
--- On Fri, 10/22/10, Lewis, Patrick wrote:
From: Lewis, Patrick
Subject: [Histonet] Coverslip removal question
To: His
Hi Histonetters
When I soak my slides in xylene to remove the coverslip, do I then need
to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and
then H20.
And then drying and re applying permount?
Or can I go straight from xylene to drying to re-coversliping with
permount?
Here's what the recently revised CAP checklist says about releasing blocks;
scroll down to Note 2:
**REVISED** 06/17/2010
ANP.12500 Record Retention Phase II
Surgical pathology records and materials are retained for an appropriate period.
NOTE 1: Minimum requirements for surgical pathology, provi
We haven't heard anything about this (and we were inspected at the end
of July). If you have any additional information, please post it.
It is a little hard to image a "locking Lid" on a disposable plastic
container of reasonable cost that is large enough to hold a a total
colon resection or large
Do you have a dissecting scope you could use? You can grab one with a
pretty low resolution camera for about $800. If you need more details
let me know.
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-29
Toni,
We will be going live with the Bond shortly and will have the same
workflow with a different LIS. Our pathologists have been ordering their
own tests for years so there is no impact, except to save time in the
Immuno Lab.
Victor
Victor Tobias
Clinical Applications Analyst
University
Does anyone use eosin in their Peloris?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Shea's
Sent: Friday, October 22, 2010 2:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] eosin in proces
Caroline
The older models of the Nikon scanner for kodachromes had an attachment that
would scan glass slides, I'm not sure about the new ones but we had one back in
the late 90's early 2000's that would hold a slide and we could scan that. You
could also take some low mag images on a microsco
This is the best kept secretFor those who have never tried this, you don't
know what you are missing in the ease of embedding and cutting.
We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100%
alcohols, leaving the last 100% pure (of course there is carry over during th
I'd be interested in knowing how this has impacted your pathologists. Were they
resistant at first? The extra time inputting requests would now fall on them.
And there is still a chance of keystroke errors with pathologist entry.
I'm not disagreeing with the comments you made, only wondering sin
Hello Everyone,
Does anyone have a good way to get low magnification images from slides? I
have some DAB stained rat coronal sections that I would like to digitize.
Basically, I want to have a picture of the entire section. It doesn¹t have
to be high resolution, just enough to make out the basic s
Does anyone know of or have access to changes in regulations for formalin
specimen containers? At our recent CAP inspection, we were told that JCAHO and
OSHA were mandating that formalin specimen containers should now have "locking"
lids.
This is the first I have heard of this.
Thanks
Ronnie
We have interfaced our Bonds with Soft Path LIS system. My
justification for this started with using it for LEAN processes in that
the orders for IHC went directly from the Pathologist to the Bond and
eliminated the need for my techs to have to input individual orders for
IHC by hand. Since we ha
We have our grossing staff cover all GI biopsies, breast biopsies and
endometrial curettings or any small colorless tissue with 0.1% basic
fuchsin. It leaves it a nice pinkish tint after processing, the specimens
are visible in the paraffin, and we haven't had any pathologist complaints.
On Fri,
I would recommend a CAP publication, "Quality Management in Anatomic
Pathology; Promoting Patient Safety Through Systems Improvement and
Error Reduction", Raouf E. Nakhleh, MD, Patrick L. Fitzgibbons, MD,
Editors.
You should be able to order it on the CAP website. It's not very
expensive and does
MPath Search Partners is looking for a Histotechncian/Histotechnologist
candidate for permanent direct hire in Wisconsin.
*Shift/Schedule:*
Monday-Friday, Day Shift Hours, 38 hours per week
* *
*Position: *Histotechnician/Histotechnologist
*Location: *
State of the art laboratory in Manit
I will be out of the office starting 10/21/2010 and will not return until
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In my absence please ask for Mary Campbell . If this is urgent or you need
to speak to me directly you can contact me on my cell phone number
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___
Hist
Depending on the size of the bone the Leica 2255 is a great mictorome to use
with tungsten carbide blades. It is heavy enough to larger blocks and is
motorized. If it is not motorized I would not try it for as that will give you
the best results.
Pam Marcum
- Original Message --
MPath Search Partners is looking for a Histotechnician/Histotechnologist
candidate for permanent direct hire in Massachusetts.
*Shift/Schedule:*
Monday-Friday, Day Shift Hours
* *
*Position: *Histotechnician/Histotechnologist
*Location: *
Pharmaceutical Company in the Burlington, MA area
Hi,
I'm looking for a good used sledge or rotary microtome capable of cutting MMA
emebdded bone specimens. Can anyone reccomend a good model, manufacturer,
supplier or know of a good used machine ?
Many thanks.
Adam
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Hi
Greetings Histologist,
Does anyone have positive or negative comments on the Thermo Citadel 1000 ? Is
there an abundance of fumes during processing?
Thanks again,
Karen
Karen E. Cruise
Histologist / Research Technician II
Washington University School of Medicine
Laboratory for Translational P
Hi Ali,
I tried FITC cd31 on mouse liver frozen section with tumors it worked really
well.
Good luck,
Anil Kumar.
On 22/10/10 11:29, "Ali Nasr Esfahani" wrote:
Hi, I am just new in this group, so I hope this is the way I can get help.
I am doing histochemistry on human hypothalamus and I ne
We are using the Sakura Prisma with attached coverslipper; it is a workhorse
and allows for the use of what ever reagents/stains that you prefer. We are
running our H&E and H Pylori stains simultaneously. We experienced a small
problem with the leveling of the instrument at the initial set up but
We have two leica XL Stainers and love them. We don't have the unit to
transfer to the coverslippers yet and that would be great but minor overall.
We are just setting up some special stains on it and the run up- run down
programs for special stains.
Pam Marcum
UAMS
- Origin
We add 1.0 mls of 10% aqueous Eosin to the first 100% Ethanol; we will then
have Eosin in all of the Ethanol containers after they have been rotated.
This removed the time consuming task of dotting each tissue at grossing and
allows for easy visualization at embedding and sectioning. I have not ha
We have a (refurbished) Lecia Autostainer XL and really like it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kendall Neely
Sent: Friday, October 22, 2010 10:32 AM
To: histonet@lists.utsouthwestern.edu
S
We put eosin in the first 100% alcohol.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Friday, October 22, 2010 11:18 AM
To: Tench, Bill
Cc: histonet@lists.utsouthwest
It sounds like I am in the minority in pitting the eosin in the *first* 100%
alcohol!
I agree with Bill, it really makes a HUGE difference in small, minute tissues.
We did run into one issue though, we were using orange biopsy cassettes and the
red-orange tissue was difficult to spot in the
For the love of your sanity (and keeping your pathologists happy)= DO
NOT BUY A SYMPHONY Their reagents are all proprietary and so
troubleshooting is almost impossible without paying Ventana to have
tech he= lp loom around your lab. The slides take 3 days or so to dry
As much as I respect Dr. Richmond, I would have to disagree that staining
bx's with eosin is a waste of pathologist time. It helps the embedding
tech and cutting tech see the minute pieces, which may be otherwise lost.
Sometimes that is the diagnostic material.
We would not want to put a patien
Hi Ali,
Although I do not ever perform endothelial immunohistochemistry in our
laboratory, I know it is common to use an antibody against CD31 to selectively
stain endothelial cells and this may work well in pituitary tissue.
Hope that helps,
Kristen
>>> Ali Nasr Esfahani 10/22/10 5:31 AM >>>
I think the use in a processor becomes a local culture. I think
everyone in my community does it (someone spread the word), so now we
all do. I believe that we are all putting it in the last alcohol. It
really makes facing a block on minute pieces a whole lot easier. We
found marking the tissue
Allison Scott at LBJ Hospital in Houston, Texas asks about the use of
eosin to dye small biopsy specimens.
Several replies mention addition of eosin to one of the processing
alcohols. I have never seen this done, in maybe 60 pathology services
I've worked in. (I'd know, because I nearly always exa
Our laboratory will be purchasing new H&E stain equipment in the near future.
We would like to hear the pros and cons out there for the Thermo-Shandon
Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in
advance for your input.
Kendall A. Neely
Histology Technical S
Mohamed,
The University of Nottingham Histology department has an extensive website of
histology protocols:
http://www.nottingham.ac.uk/pathology/default.html
There is a protocol for PAS/OG on that site. I do not have any experience using
it, but as it comes out of John Bancroft's old lab
---
dear all
can you share me pituitary stain protocol called
PAS/ celestine blue / Orange G?
thanks in advance
mohamed
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We use eosin at the grossing bench on endoscopic specimens and hematoxylin
on prostate/liver biopsies. The pathologist drops a drop of stain on each
specimen.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 2380
We use cobalt blue as it does not interfere with FISH
Sent from my Verizon Wireless Phone
- Reply message -
From: susan.wal...@hcahealthcare.com
Date: Fri, Oct 22, 2010 3:28 am
Subject: [Histonet] Eosin to dye small Biopsies
To: ,
Cc:
We use about the same amount but in our last 100%
Hi, I am just new in this group, so I hope this is the way I can get help.
I am doing histochemistry on human hypothalamus and I need to stain
endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as
the whole and like a line. But I would like to have an image of the
endothelial
We use about the same amount but in our last 100% Alc.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Thursday, October 21, 2010 4:40 PM
To: Scott, Allison D
Cc:
Subjec
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