Re: [Histonet] rapid h and e

We used to do the similar to what Bill Tench described below. However, destaining and restaining with Pap caused loss of some of the cells and the pathologists prefer the pap for these smears so we devised a Stat Pap stain: from alcohol, 50% alcohol, water, Gil's Hematoxylin for 1 min, water, blu

[Histonet] rapid h and e

We use the same materials and timing we use for a frozen section, which as a cytopathologist works just fine for me. Approx 1 min Hematox, several dips wash, dips in blueing UNTIL BLUE (people tend to shorten this critical step), no more than 5 dips in eos, 100 ETOH X 2, xylene dipped until runs s

[Histonet] Refurbished histology Leica equipment

Hello, I would like to know if anyone has had any experience using a refurbished Leica XL auto stainer and coverslip CV5000. Any thoughts you would not mind sharing? Thanks in advance, ___ Histonet mai

[Histonet] Routine histology equipment

Hello, I was wondering if anyone who is using the ThermoFisher histology equipment would mind sharing any thoughts regarding their experience with this equipment. I'm looking at the Excelsior processor, gem ES H&E stainer, clearvue coverslipper, print mate and slide mate (cassette and slide

histonet@lists.utsouthwestern.edu

Our pathologists want a rapid H&E stain to set up in CT to determine the adequacy of lung FNA's. They do not want a diff quik stain. Any suggestions on something fast and easy that doesn't require a lot rinsing? Laurie Colbert ___ Histonet mailing

RE: [Histonet] Waste down the drain

Great advice Erin! We did the same thing with the waste from our Dako Artisan to document the percentages of trace metals. Again, they were too low to require special handling over and above what we normally do with our hazardous waste. Steve -Original Message- From: histonet-boun...@

Re: [Histonet] Waste down the drain

The EPA will not agree to biohazardous waste down the drain even if a = local municipality does. If the EPA inspects a facility dumping biohazardou= s waste down the drain it will be a hefty fine and surprise inspections for= ~2 years. I've know 2 facilities that were

AW: [Histonet] question to ventana benchmark XT and ultra

Hi Ashley, the clearance is between 1-3 mm and I think that's too small. In a few days Ventana will lift the arm with the hammer for additional 3 mm. That should help for a reliable working of the instrument. Half an inch is about 12 mm. I think, not even with the detection-kit-preps this clearance

[Histonet] Formalin

Absolutely not!!! In California. Safety office picks it up and dispose of it. Michelle Aloni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Minotome cryostats

Hello, I would to know if anyone knows how to get two Minotome cryostats apraised. They were manufactured by Damon/IEC Division and remanufactured by Belair Instruments. Thank You, ___ Histonet mailing list

[Histonet] RE: Vibratome sectioning of mouse spinal cord

Hello Susan, Before I can help, I need more information from you. Are your samples fixed or unfixed? In any case, make sure that the vibratome is in working order & that all the movable parts are tight before you section on the vibratome. In addition, during cutting the holding tray should ha

RE: [Histonet] question to ventana benchmark XT and ultra

You might not have seated the top of the prep kit container all the way down. They should not be different heights. I would take each of them and press down on the top to make sure the lid is on properly. If you set them side by side and they are still different heights, I would contact your

RE: [Histonet] RAGE and AGE

RAGE and AGE - yes rage gets worse with age...it's called menopausei just had too it's FRIDAY!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Annette Featherstone Sent: Friday, November 12, 2010 12:19

[Histonet] RAGE and AGE

Is anyone using RAGE or AGE antibody successfully? If so would you be willing to share vendor and data? I am also looking for a Tunel Assay kit, what would any of you recommend? Thanks, Annette Featherstone ___ Histonet mailing list Histonet@lists

[Histonet] Waste down the drain

In reading all the comments about waste down the drain, proprietary ingredients, etc., keep in mind that disposal regulations are different everywhere and NO vendor will tell you anything other than to follow those regs and/or play it safe and tell you to haul it away. At a previous employer in

AW: [Histonet] question to ventana benchmark XT and ultra users

Thanks Clare, Since we know the problem, we always press the lid very strongly down. And with the XT we were able to react sometimes, when we heard the clacking sound, and cut off the lid while the instrument was running. The Ultra is closed. I ask because the people of Ventana never heard of such

RE: [Histonet] question to ventana benchmark XT and ultra users

Make sure that the lids on your prep-kit dispensers are pushed all the way down. Since the space between the hammer and the tops of the dispensers is so small, if one lid is not pushed down all the way the hammer will grab it. Seen it happen many times myself! Clare J. Thornton, HTL(ASCP),QIH

Re: [Histonet] question to ventana benchmark XT and ultra users

We have had the ultras for over a year now and that is a new one. Was the rack of antibodies not seated properly? Just a thought. Stacey Sent via BlackBerry from T-Mobile -Original Message- From: Gudrun Lang Sender: "histonet-boun...@lists.utsouthwestern.edu" Date: Fri, 12 Nov

[Histonet] question to ventana benchmark XT and ultra users

Hi! This week we've got the Ultra. In the first run it happend, that the "hammer" touched a Prep-Kit from the side while rotating and pulled the whole reagens-rack down. (That makes a very uncomfortable noise.) We saw, that the distance between the hammer and the Prep-Kits is very small, and it d

RE: [Histonet] Formalin

Not here in Atlanta until it it neutralized. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J. Sent: Friday, November 12, 2010 10:46 To: Laurie Colbert; godsgal...@aol.com; Histonet@lists.uts

RE: [Histonet] Formalin

No way in Florida!!! From: histonet-boun...@lists.utsouthwestern.edu on behalf of jmacdon...@mtsac.edu Sent: Thu 11/11/2010 7:45 PM To: godsgal...@aol.com; histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] F

RE: [Histonet] Formalin

Not here in The Netherlands. Van: histonet-boun...@lists.utsouthwestern.edu namens Laurie Colbert Verzonden: vr 12/11/2010 15:42 Aan: godsgal...@aol.com; Histonet@lists.utsouthwestern.edu Onderwerp: RE: [Histonet] Formalin Not us - in Pasadena CA. -Origina

RE: [Histonet] Formalin

Ditto in Massachusetts. I work at MGH and we are striclty regulated by the MWRA (Mass Water Resources Association) which monitors closely anything that goes down the drain. They are especially concerned with mercury and the ppm are very minute. Anything going down the drain can affect Boston Ha

[Histonet] Needle Core Bxs

Good Morning,  Does anyone have a manual hand dip method to process needle core biopsy tissue?  Also looking for a good microwave method as well for a Thermo Shandon Tissue Wave 2. Thanks,  Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267

RE: [Histonet] Formalin

Not us - in Pasadena CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of godsgal...@aol.com Sent: Thursday, November 11, 2010 2:45 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok, s

[Histonet] Histo data needed

A big thank you to everyone who responded to my post. All of the responses were helpful! - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 ___ Histonet mailing list Histo

RE: [Histonet] Histology Supervisor Job in Oklahoma

Hi Alisha Thanks for the information. Starting in January, if you want to forward your postings to my work email at jweav...@cscc.edu I can also post them on our histology program web page and learning platform for histology students. This information can also make it to experienced histotechs

Re: [Histonet] CK7/EMA control tissue

Some lung carcinoma could be positive for both. Since IHC is usually done on tissue with cancer, which often has low levels of antigens, I personally would rather use another cancer as a control, rather than a "normal/benign" tissue which would have high levels of the antigen. More likely to m