speaking of,
I guess my last posting rustled some feathers. I received a phone call from
my rep saying that upper management wants to talk to me. I told him that
there wasn't anything to talk about. Except that I was remiss. I want to
thank Chris for all her work in the lab. I'm sorry that I di
That's the way it normally looks, kinda globby. ORO is really not that
soluble in fat, just more soluble than the prop. glycol.
Jay A. Lundgren M.S., HTL
(ASCP)
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Get rid of all your recycled reagents. Try running new reagents all
the way though. Also, increase your rinse times and agitation in the
rinse. I'll bet this will solve your problem.
Jay A. Lundgren M.S.,
HTL (ASCP)
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Hi Histonetters!
I hope everyone is having a great day. I wanted to take just a minute
of your time to tell you about some histology opportunities that I am
working on. All of my positions are full time permanent jobs. My
clients offer excellent compensation, benefits, relocation assistance
and
HISTOLOGIST - Mass Histology Service, Inc. is a busy, well established,
rapidly growing, highly reputable private, GLP-compliant histopathology lab
located in central MA. We are looking for an experienced histologist with a
strong background in routine and special stains, frozen sections,
immunohi
Dear Histonet Community,
I hope all is well with you since we were last in touch. As a reminder I am a
one of the founders of a healthcare recruiting firm. I help Lab Professionals
find permanent employment and I wanted to see if you are interested in
exploring other career opportunit
Looking for experienced histology technicians and cytotechnologists in
Columbus, Ohio for new GU pathology laboratory. Excellent compensation with
great benefits. Experience with IHC, FISH, and GU pathology a plus. Send
resume or CV to care...@aksm.com.
_
The Methanol concentration of Formalin, 37% is frequently described on
the MSDS as 10-15%. However, I believe the actual Methanol
concentration is normally at the low end of that range. Both
Formaldehyde Gas and Methanol are Flammable.
Commercial 10% NBF typically contains about 1.1% Meth
Temps MIGHT be different. Do they have doors? What's behind the wall
(poorly insulated, hot water pipes) All of that being said, the point
might be are they within a range? Most likely they are, especially since
the lab establishes the range. 5 degrees Celcius on either side of 25
should keep every
All,
Hershey Medical Center in Hershey PA, is looking for a
Histotechnologist with EM experience for their EM lab. Candidate may
have EM experience in research but must have at least an Associates
Degree in one of the sciences and some histology background. If anyone
is interested, please conta
Serra's fixative does have water in it, the water is from the
formaldehyde. 37% formaldehyde is water saturated with formaldehyde gas
to about 37%.
The precipitate is probably from the buffering compound, phosphate perhaps.
Geoff
Emily Sours wrote:
A related question:
I was making Serra's fix
A related question:
I was making Serra's fix (which has no water in it) and I tried to use
buffered formalin. This turned the solution white (which happens when
Serra's has water in it). Another brand of formalin (which did not
say it was buffered) worked fine.
What's in the buffering that has wa
Hi Everyone
Posting this for a colleague who is doing oil red o staining on carotid
plaques and is seeing both large globular and abundant fine small globular
staining. Localization is correct but she is concerned about the amount and
size variability of the staining pattern. She has been u
Wouldn't all your cabinets be the same temperature as that of the room
the cabinets are in? We just monitor the general ambient temperature
because some of our reagents state that storage is at room temperature.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-2
We are replacing out Ventana XT with an Ultra. We are currently running our
ER's and Pr's on our Ventana XT. The tech doing the Immuno's told me she
cannot do them on the Ultra because it has not been approved yet. We
currently are not using a disclaimer on the Er/Pr reports because these
have b
Good Morning,
We are looking for a Working Histology Supervisor at UAMS in Little rock AR.
We are also looking for two histologists one for routine surgical pathology and
one for the IHC area.
There will be rotation in the future between Histology and IHC.
If you are interested
Good Morning,
We are looking for a Working Histology Supervisor at UAMS in Little rock AR.
We are also looking for two histologists one for routine surgical pathology and
one for the IHC area.
There will be rotation in the future between Histology and IHC.
If you are interested
Commercially purchased 37% formaldehyde has had a small amount (about
1.5% I think) of methanol added to it for many, many years. It helps to
prevent the polymerization of formaldehyde into insoluble
paraformaldehyde. It certainly does not make the stock solution
flammable and it is not contrib
ANP. 21390 Reagent Storage
All reagents are stored as recommended by the manufacturer.
The evidence of compliance now states you must show records such as
refrigerator, freezer and room temperature monitoring as applicable.
We keep records of the refrigerator temps used to store our IHC but we ha
We keep our 2 years.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Beha
Hello everyone,
Could someone please tell how long we are required to keep the patients'
pathology requisitions in the lab, after the case has been read or signed out.
Thank You!
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I guess the producer of the NBF has to state the Methanol-content, because
37% Formaldehyd (stocksolution) usually is made stable with an amount of
methanol.
That does not influence the fixation performance.
There's a study by Fox about this. And he saw shrinkage of tissue only when
using the conce
The ink also stays on through decal solutions which we needed. We do 30 bone
marrows a day any system that could not stand up to decal was a no go for us.
Pam Marcum
UAMS
- Original Message -
From: "Laurie Colbert"
To: "Pamela Gholston" ,
histonet@lists.utsouthwestern.ed
Greetings to all of you in Histoland. Our lab recently switched from using a
formalin substitute to using 10% Neutral Buffered Formalin. Our Pathologists
have been unhappy with the small tissues, like GI biopsies and prostate cores.
They say they are seeing too much chatter and poor nuclear d
I have the Printmate and 5 Slidemates/PSLIMs. The hot foil tape works
fine for us - the print is chemically resistant. We use xylene for both
processing and staining.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouth
Hello Histoland,
if there is anyone who can send me a digital picture of murine urinary
bladder, H&E stained, Bouin-Hollande fixed if possible - please do so!
Thank you,
Valentin
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I have to agree with Sheila's statement. When we experience this during
fish or immuno staining my first thing to check is temperature and the next
thing is the slides. The slides almost always is the culprit.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hos
We recently had problems with our Superfrost Plus slides that we purchase from
Cardinal. We had sections washing off and uneven staining. We switched to VWR
VistaVision Histobond Slides and have had no further issues.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
Hello,
We are a lab that has been running Pathvysion Her2 FISH for years
without any major problems. Recently we have been experiencing more and
more tissue section loss. I have noticed that some of the loss is
starting during pretreatment. My temperatures for all steps are what
they should be.
I would like to thank all of you that sent me information regarding
the Ventana Symphony. It appears from the dozen of you that sent me
information, you were all pretty consistent in your assessments. It
was exactly what I needed and I now feel confident to attend my meeting.
Thanks a bun
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