[Histonet] infectious fixed tissue and cells

2011-02-22 Thread IRENA SREBOTNIK KIRBIS
Dear all, could anyone out there provide a scientific reason for wearing gloves handling slides with FFPE sections and FIXED cytology slides? I could understand that by wearing gloves we could prevent epithelial contamination of cytology slides but I would like to know which microorganism could

[Histonet] Sirius red stain - picric acid substitute - water - really?

2011-02-22 Thread Mia Woodruff
Hello all, I have been undertaking sirius red staining using picric acid, I was under the impression (from papers) that picric acid is an important component of the procedure and read that it prevents non-specific binding of the dye to things other than collagen. However, I have recently found

[Histonet] HURRY the GSH meeting deadline for hotel reservations is TOMORROW

2011-02-22 Thread Shirley A. Powell
Reminder for ALL histotechs, Last day before deadline at the Mountain Creek Inn tomorrow, the 23rd. The actual meeting registration has no deadline, but the prices will go up at the hotel and rooms are going fast. Call today, and definitely by tomorrow. The Georgia Society for Histotechno

RE: [Histonet] ARTISAN STAINER - DAKO - WASTE DISPOSAL

2011-02-22 Thread WILLIAM DESALVO
We use Clean Harbors Hazardous Waste Disposal to remove the liquid waste in total. The Artisan's have their own waste stream and container. William DeSalvo, B.S., HTL(ASCP) > From: karen@albertahealthservices.ca > To: histonet@lists.utsouthwestern.edu > Date: Tue, 22 Feb 2011 12:35:0

[Histonet] RE: ARTISAN STAINER - DAKO - WASTE DISPOSAL

2011-02-22 Thread Weems, Joyce
We have our picked up by a hazardous waste company. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histone

[Histonet] ARTISAN STAINER - DAKO - WASTE DISPOSAL

2011-02-22 Thread Karen Kay
Hello, A question for Dako's Artisan Stainer users as to what you are doing with the Waste disposal and in particular the Trace Metals waste. Are you neutralizing it and if so, could you provide proportions of waste to 0.85% NaCl? We have recently started using the instrument and are trying to

[Histonet] LCM & archival FFPE tissue

2011-02-22 Thread Margaryan, Naira
Hi all, I have a couple questions I would like to throw out to the experts: 1) 1) Is it possible to extract RNA and DNA for gene expression purpose after usual IHC procedure on 4um sections of archival FFPE breast tumor tissue (If tissue was in citrate buffer, pH 6.0; several blocking step

[Histonet] Remedial action plan

2011-02-22 Thread rmweber113
Hi,  Does anyone have a procedure for a Remedial Action Plan for histology/cytology they are willing to share? Thank you, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC

[Histonet] Histonet Digest, Vol 87, Issue 38 fixed cytology slides

2011-02-22 Thread Lecorchick, William
Message: 15 Date: Tue, 22 Feb 2011 18:34:26 +0100 From: IRENA SREBOTNIK KIRBIS Subject: [Histonet] fixed cytology slides To: , Message-ID: Content-Type: text/plain; charset="iso-8859-2" Greetings to all, so far we handled wet-fixed cytology slides as health risk free without gloves, h

RE: [Histonet] fixed cytology slides

2011-02-22 Thread sgoebel
YES!! Wear gloves!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Beha

[Histonet] fixed cytology slides

2011-02-22 Thread IRENA SREBOTNIK KIRBIS
Greetings to all, so far we handled wet-fixed cytology slides as health risk free without gloves, however there is no data regarding this on the net (or at least I wasn't able to find it). I would appreciate response on safety policy in other cytology labs regarding fixed cytology slides and/or

[Histonet] Medicare Billing Help new rules 2010

2011-02-22 Thread Matthew Lunetta
Hey all, I am having problems finding in the archives the link for the new Medicare pathology rules. Would someone please post it again. I have also tried the Medicare web site and it is not very user friendly. Thanks for the help Matt Lunetta HT (ASCP) _

RE: [Histonet] Decal Solution for Bone Marrow Core Biopsies

2011-02-22 Thread Johnson, Nacaela
We use BBC Rapid-Cal Immuno for 90-120 minutes. It works great and does not deplete iron stores or affect immunos. Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.j

[Histonet] Per Diem needed in Orange County, California

2011-02-22 Thread Paula Lucas
Per Diem histotech needed for vacation coverages and occasional Saturdays. If interested, please contact me and I'll give you more information. Thank you, Paula Lucas Bio-Path Medical Group Fountain Valley, CA 92708 714-433-1330 ___ Histonet mailing

Re: [Histonet] Formalin-fixed paraffin embedded question

2011-02-22 Thread Grantham, Andrea L - (algranth)
I'd be happy to help. Maybe you can too! 8-) On Feb 22, 2011, at 8:53 AM, wrote: > Are you volunteering? That is so nice of you =) > > Sarah Goebel, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > >

RE: [Histonet] Formalin-fixed paraffin embedded question

2011-02-22 Thread sgoebel
Are you volunteering? That is so nice of you =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.u

RE: [Histonet] Formalin-fixed paraffin embedded question

2011-02-22 Thread sgoebel
Just got to thinking...I do animal tissue processing. I do use an automated processor, but it is open with no pressure or vacuum. If you think this will help I can give you the times I use. It is an overnight process and takes more than a work day worth of hours. Let me know? Sarah Goebel, BA,

Re: [Histonet] Formalin-fixed paraffin embedded question

2011-02-22 Thread Grantham, Andrea L - (algranth)
The NSH Manual needs to be seriously updated. There is no protocol for mouse brain that could help this guy with his problem. Andi On Feb 22, 2011, at 2:02 AM, Margaret Blount wrote: > I agree with all the comments so far, and I think all the steps are far > too long, particularly the overnig

[Histonet] New Mexico HT Position

2011-02-22 Thread Hale, Meredith
Great opportunity for a Histotechnician in a brand new laboratory! Digestive Disease Consultants of Las Cruces , NM is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory exp

[Histonet] NYSHS Meeting

2011-02-22 Thread Amy Farnan
Hello vendors! The NYSHS State Meeting is being held May 14th at the Desmond in Albany. If you would like to represent your company at our meeting and rent a booth please contact our vendor co-ordinator Linda Chen at kook...@hotmail.com for more information. Thank you Disclaimer: The informa

[Histonet] Re: Tissue Processors

2011-02-22 Thread L White
Hi Joe, We just completed an extensive tissue processor evaluation. We have an Excelsior and use it exclusively for biopsies. It is a good processor with very little hands-on maintenance, with the one exception that you cannot access the reagent bottles for regular cleaning. This becomes a probl

[Histonet] Re: Decal Solution for Bone Marrow Core Biopsies

2011-02-22 Thread Robert Richmond
Marcia Fisher at El Centro Regional Medical Center (at the southeastern tip of California) asks: >>We would like to find another type of decal solution for our bone marrow core >>biopsies. The current solution, which is also used on routine bone specimens, >>is leaching out the iron deposits. Wh

RE: [Histonet] Formalin-fixed paraffin embedded question

2011-02-22 Thread Margaret Blount
I agree with all the comments so far, and I think all the steps are far too long, particularly the overnight in molten wax. You are effectively cooking your samples. I would avoid overnight in 100% ethanol too; it will tend to harden the tissue. Get hold of the manual for animal tissues available f