Rae
We have, you need standard training, but the individuals who inspected our lab
did not mention compentances. But our inspection happened because we needed to
switch from a conditionally exempt small quantity generator to a small
quantitiy generator and we then needed to register with the
Hi Mia,
I tried few different sources of Picric acid. Unfortunately, not all of them
worked. The one that is working is from Rowley Biochemical Institute -
www.rowleybio.com . I got a small bottle to try - 4 oz. Cat # SO-128. You
can probably find it from one of the large vendors.
Just a h
Hi Mia,
In response to your question about the need for picric acid in the
sirus red solution. Yes, you do need it. The article you listed was working
with collagen gels and not actually tissue sections. If you omit the picric
acid you not only loose the yellow counter stain as mentioned in th
Has anyone been inspected by the EPA regarding removal of hazardous
chemicals? It came up in a safety meeting that I should have training and
competencies (beyond general safety training and PPE) for pouring xylene
from staining dishes and processors into accumulation containers.
Rae Ann
I wouldn't decolorize the slides. Just remove the coverslip and hydrate to
water. Any antigen retrieval should take out the Eosin. You're going to
counterstain in Hematoxylin any way. Years ago (ok, many moons ago) I did an
informal study. I took 10 antibodies, stained two sets of controls H & E
Cervix (with both endocervix and ectocervix) contains both glycogen (ecto)
and mucin (endo).
Jennifer
Tony Henwood
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/23/2011 01:56 PM
To
"'Kuhnla, Melissa'" ,
"histonet@lists.utsouthwestern.edu"
cc
Subject
[Histonet] RE: alcian blue-PA
Yes, you can use H&E-stained slides for IHC; however, if your lesion/tumor is
negative and there is no internal positive control, then you may be dealing
with a false negative result. Having said that, we have had good luck with the
majority of proteins we have tested.
Richard
Richard W. Cart
Melissa,
We use a multi-tissue control.
Our control block contains appendix, umbilical cord and liver (for diastase).
The optimum control (if you can get it) would be an intestinal metaplasia of
the stomach and combine this with a mucin-producing mesothelioma.
To you all, any further ideas?
Re
Jo-Ann,
Nothing is changed, but storage in PBS does not affect neither morphology nor
antigens. I used to keep some neutral formalin- or formaldehyde-PBS fixed
specimens up to 1 year in sterile PBS at +4 C without any visible changes. So
- don't worry. It will be fine.
Anatoli Gleiberman, PhD
Nothing has changed, it is just "one of those things" people do because of
fundamental ignorance.
PBS is not a preservative and could even favor bacterial growth (depending of
the time elapsed).
After proper fixation, tissues should be placed in EthOL 70% as a general
practice.René J.
--- On
Hi All,
It has come to my attention recently that some of our histology clients are
submitting their samples in PBS after fixation, instead of 70% alcohol. When I
ask them why they don't know and say they have been told to. I have always
followed the procedure that after fixation specimens a
Cindy
We have three dako autostainers and we really like them a lot. We are a
contract research lab so flexibility and system openness is key for us.
The only other instrument that I have demoed is the Leica Bond, but
unless I purchased the research software (which is a bit expensive) I
was tied
So...trying to find someone who sells RRAS antibody that isn't Abcam. I
can't seem to make the Abcam antibody work and I have been asked to try
and get it from a different company.
2. Does anyone have any experience with RRAS? I have tried dilutions
from 1:500 all the way up to 1:2000. I ke
It will work and if you are performing antigen retrieval then you won't need to
decolorize the slide, just rehydrate and perform per you usual protocol.
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen B
Robin,
We just take the coverslip/film off, hydrate to water and run for IHC
BUT many times the H&E slide is not a "+" slide so we end up losing
tissue. It is not necessary to destain the H&E first.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Hig
The Joint Commission web site:
http://www.jointcommission.org/accreditation/accreditation_main.aspx
click on Standards and then click on National Patient Safety Goals. It
is the first standard in the chapter.
Standard number: NPSG.01.01.01
Debbie M. Boyd, HT(ASCP) l Chief Hitologist l Southsi
I did notice CAP inspectors concentrated more on safety this time around.
>>> Akemi Allison 2/23/2011 11:50 AM >>>
We were given the new CAP checklist. I totally revamped our SOP Manuals, ALL
QC
forms, etc. to comply.
This is the 1st Childrens Hospital CAP inspection I have undergone. We
Hi all,
Is it possible to de-stain H&E stained paraffin sections and re-use them
for IHC staining??? Does anything special have to be done? Will it only work
for some epitopes/stains? Does anyone have suggestions on how to do this? I
am being asked to do this and don't want to waste my time if
Hi Histonetters
I currently use a Dako stainer for my IHC staining. It is a work horse with
very little problems. It is a older model that we may need to replace in the
near future. What is everyone using out in histoland. I would be perfectly
willing to purchase another Dako but I want to explore
Hi Histonetters!
I have a question related to the two patient identifiers that TJC requires:
can anyone point (online) me to the actual regulation?
It was my understanding that the 2 identifiers related to the *collection*
of the specimen, meaning that the container and associated requisiti
If your lab smells like oranges, just open your CAP window and air it
out.
Sincerely,
Jay A.
Lundgren M.S., HTL (ASCP)
Hello All,
Does anyone use a Diff Quik protocol for parasites in stool samples and if so
could you send me a copy of your procedure? I would greatly appreciate it.
Thank you all.
Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. | McKinney, TX 75071
Di
Are your blocks (1 bx and 1 clot) separate specimens, or just one specimen with
2 blocks?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Goodwin,
Diana
Sent: Monday, February 21, 2011 11:40 AM
To: 'histonet
I have used archival FFPE slides that had used antigen retrieval methods for
LCM and the yields seemed to be good. The RNA/DNA yields obviously depend on
how many cells you are collecting. We routinely used 8um sections for the LCM
to increase yields. If you are interested in collecting a pop
We were given the new CAP checklist. I totally revamped our SOP Manuals, ALL
QC
forms, etc. to comply.
This is the 1st Childrens Hospital CAP inspection I have undergone. We are
inspected by other Children Hospital inspecting teams.
After all the hard work, they did not go through any
Andrew
We are a research contract lab and have been scanning slides (WSI) since
2007. We have an Aperio Scanscope XT. Our clients have secure remote
access to their images. We manage our own server, storage (20TB) and
backup locally.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Prem
That's a good thing! That means you need more ventilation and admin
listens when inspectors speak!
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
We currently use Cat#Z2001, clone 13H4 from Zeta Corp with great
results, although we only use it for HMWCK/p63/P504s stain. We have
also used a predilute (PP200) from BioCare (that worked quite well).
Sally
From: ih...@googlegroups.com [mailto:
Hi Everyone,
Our pathologists are interested in using this antibody to detect metastatic
renal cell carcinoma in paraffin sections. They are not interested in an
antibody cocktail, but also want to use it to detect prostate cancer. I'd like
to know what company and clone most labs are using.
T
What?!? A histology lab with no enough space...who ever heard of such a
thing
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[m
Sometimes it is a good thing when CAP says you are cramped for space. It
"sometimes" makes the hospital Administration wake up and take notice!!!
W
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
We just had ours last week.
We did well, but the inspecting Pathologist said the lab smelled like oranges
we were in a way too cramped space. Go figure..
>>> Akemi Allison 2/23/2011 9:02 AM >>>
Lot's of Labs in LA are in their CAP window! We had our CAP inspection
yesterday and having our s
Akemi, were you inspected with the new CAP checklist or the old one?
The situation in our lab is that we were given the checklist and then
they changed us to the new format. Your thoughts
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.uts
Since you can buy a saturated Picric acid solution and I have for years why are
you worried? It is stable in a saturated liquid and you can purchase fairly
small amounts at a time. I only worry if find some in a powder form turning a
nasty colour. It has been about twenty five years since I h
Akemi,
Keep us informed!!! I too, am in my CAP window!!!
Thanks,
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
This email and any files transmitted with it may contain PRIVILEGED or
CONF
I would do a trial run on "extra" slides or non-valuable tissue?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff
Sent: Tuesday, February 22, 2011 10:33 PM
To: histonet@lists.utsouthwestern.edu
Lot's of Labs in LA are in their CAP window! We had our CAP inspection
yesterday and having our summation this morning at 9:00. I think our
department
did pretty good. Keeping my fingers crossed.
Akemi Allison BS, HT(ASCP)HTL
___
Histone
Dear Histonet,
Please don't take this as spam. I am just gathering some information...
I am curious to know if any of you are currently working with digital
whole slide scanners and what your experience has been to date.
Additionally, my company provides telepathology services by putting a
Liz,
You can perform IgG and IgM on FFPE tissue samples. We do them through IHC
on our Ventana Ultra and get the antibodies from Cell Marque. Our
pathologists are happy with them.
Sheila
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.
Can anyone recommend tissue besides duodenum??
_
From: Kuhnla, Melissa
Sent: Wednesday, February 23, 2011 7:37 AM
To: ' (histonet@lists.utsouthwestern.edu)'
Subject: alcian blue-PAS control
Hello,
Can anyone recommend a good control tissue to use for A
Hello,
Can anyone recommend a good control tissue to use for Alcian blue-PAS?
Melissa Kuhnla
Lead Medical Technologist for IHC and FISH
Catholic Health Services of Long Island
Regional Laboratory Services
The information in this e-mail, and any attachments therein, is confidential
and for use
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