We do a short run some mornings for shaves and small punches. Keep in mind
that we don't work 24/7, so these have been sitting in formalin overnight.
20 min formalin
1 min distilled water
10 min 80% alcohol
10 min 90% alcohol
10 min 95% alcohol
10 min absolute alcohol
20 min absolute alcohol
10 m
90 minutes for up to 2mm thickness using K3000 or VIP5.
I do not have a VIP6
Steve A. McClain, MD
McClain Laboratories, LLC
45 Manor Road
Smithtown, NY 11787
631 361-4000
631 361-4037fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@list
I have had graduates from my program that are "mature". They have not had
problems getting jobs, or advancing once hired. That is true for either
gender. A good tech is a good tech.
Jennifer MacDonald
Mt. San Antonio College
"Mary"
Sent by: histonet-boun...@lists.utsouthwestern.edu
04/2
Another culprit is static. Moisture helps to keep the static down.
"Clough, Bret"
Sent by: histonet-boun...@lists.utsouthwestern.edu
04/21/2011 12:11 PM
To
Histonet list serv.
cc
Subject
[Histonet] How to keep paraffin sections from sticking to blade/blade
holder.
While sectioning
What’s the chance of getting a job in histology as a new grad at age 57. Will
gender be an issue? Thanks for the input. Jason
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Hello,
I work for a Mohs surgeon who wants to establish key performance indicators
(KPIs) for the mohs techs to evaluate daily performance. Does anyone have any
information of surveys or standards for the number of recuts per day or per
block as a measure for quality Mohs sectioning? She's wo
The endogenous peroxidase has to be blocked (quenching others call this step)
only before the procedure because the idea is to avoid the interference of the
peroxidase with it.
René J.
--- On Thu, 4/21/11, Emily Sours wrote:
From: Emily Sours
Subject: [Histonet] endogenous peroxidases
To: hi
Hello
I know I can block endogenous peroxidases in paraffin sections before
antigen retrieval or after it, but which is better?
Does it make a difference?
Also, since our H2O2 is 30%, do I need to account for the water in it when
I'm diluting it in 100% MeOH?
(I was going to use a 0.3% H2O2 in MeO
Open a beer to the science gods.
It may also help to follow the other instructions sent to the histonet list.
Emily
A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron
On Thu, Apr 21, 2011 at 3:07 PM, Clo
Keep everything cold, cold, cold. The block, the blade, the blade holder.
Cold spray the blade and blade holder, cool the block on ice. Warning: Use
cold spray sparingly on the block, you can ruin your tissue with too much.
Hullabaloo, Caneck! Caneck
1- change cutting angle
2- keep the blade holder clean (without paraffin)
3- make sure the blade is not blunt
René J.
--- On Thu, 4/21/11, Clough, Bret wrote:
From: Clough, Bret
Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade
holder.
To: "Histonet list serv.
Carol
We are a research lab but we have always processed our skin samples on
longer processing cycles, even the punch biopsy samples. The shaves
might be different, if I wanted to cut down I would start with how you
normally process them and then cut down at 5 minute increments to start
and see h
Yes - make sure they're long enough in the SEAT! Mine roll around the
lab like little 5-wheeled rockets, but cut off the circulation to my
thighs (is that something I can say on television?). And no arms on the
chairs!
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While sectioning paraffin embedded tissue on a rotary microtome I noticed the
sections sticking to the blade holder and bunching up. What do I need to do so
that my section come off the blade in a ribbon? Please note that this is my
first time in sectioning tissue and this is a learning exercis
We are currently in need of some ergonomic chairs for our embedding and
microtomy stations. Does anyone have any
informatio to share in reguard to chairs?
__
The contents of this message may contain private, protected and/or pri
Hello,
Does anyone use the Leica St4020 stainer? If so, how do you have the stainer
set up and how many slides do you run on the stainer before you change
reagents? Which reagents do you change and which do you not?
Thanks,
Trini
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You could always get formical which fixes and decals at the same time. This
would eliminate the waiting for fixation. Although I do usually let it fix in
straight formalin overnight first.
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Te
We do a lot of IHC staining on bone and cartilage samples and we use 10% formic
acid for decalcification for immunohistochemistry. I'm afraid you may not be
able to turnaround this in a week. The tissue needs to be adequately fixed
prior to decalcification, and properly decaled. I would trim
Try dehydrating longer. If there is water left on the slide, the eosin
will bleed.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christi
Cosby
Sent: Thursday, April 21, 2011 9:18 AM
To: his
Hi Cindy-
We have cyto prep/lab asst. position that performs pap and non-gyn preparation.
Cytotechs screen and HPVs are sent out.
Nancy Schmitt HT, MLT(ASCP)
Dubuque, IA
Date: Thu, 21 Apr 2011 09:51:35 -0400
From: "Cynthia Pyse"
Subject: [Histonet] cyto prep
To: "'Histonet'"
Message-ID: <0
Hi from Southern Cali,
We neutralize our formalin with a product called Neutralex. It comes in powder
form and after 30 mins we can dump it down the drain. We also keep
records for QC purposes. Product is purchased from a company called American
MasterTech. Hope this gives you a starting place.
This happened to me a couple weeks ago. You got water on the slide
after the eosin staining. Just run it back through alcohols, and rinse
for about five minutes. Change out the alcohols, and re-stain.
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Is anyone out there in Histoland Neutralizing their formalin before discarding
it down the drain? We are looking into doing this because of our water
authorities and we are finding that it is very costly. Has anyone found a
cost efficient way to do this? I would need to know what supplier y
Hi,
In our lab we are having trouble with the eosin bleeding off the section
after coverslipping. We are performing a rapid H&E stain on frozen
sections. Any advice would be greatly appreciated!
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--On Thursday, April 21
We no longer do Gyn, but when we did we had lab assistants do the prep. Have
lab asst for non-Gyn prep currently. J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
Fr
Jupiter's thunder
two people in a row? really?!!?!?
LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU
SUBSCRIBED ON.
Both of you are officially banned from the internet. Forever. Don't use it
again.
And don't EVER join a mailing list. EVER.
Emily, who would love t
We have lab assistants running thin preps.
Our cytotechs screen and do HPV.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
From: Cynthia Pyse
To: Histonet
Sent: Thu, April 21, 2011 9:51:35 AM
Subject: [Histonet] cyto prep
Good Morning Hist
Would anyone be willing to share their IHC menu with us? I want to see how we
compare with other top-class facilities. Obviously I am more interested in
pediatric facilities, but we are constantly evaluating antibodies for research
studies, and what may have applications in pediatric pathology f
Hello everyone!
What is the shortest cycle you may be using for processing skin specimens? Can
skin biopsies, shaves, and punches be processed well on a short run on the VIP
6? I need to find a successful protocol if possible to run skins on a 6 hour
process, if possible.
Thank you in advance
Good Morning Histonetters
For those of you who have pap smears in their facility's, who do you have
preparing the pap smears. Lab assistants, lab tech, or lab technologists? I
am having a discussion with my general manager on who can prep pap smears. I
decided to take a quick poll to see what ever
Hematoxylin should not affect the immunoreactivity (since counterstaining
occurs following the IHC reaction), so no, changing the hematoxylin would not
require a revalidation. Just make sure you are not obscuring the immunostain
with an overly-dark counterstain. The point is to provide contras
Hi Histonetters,
I was hoping those of you who may have worked with UCP1 antibody might be
willing to share how you optimized this antibody. I am using UCP1 antibody
(ab10983) from Abcam. I have followed the datasheet and have used both protease
and proteinase k and at varying times with no re
Hi,
I have some frozen house hearts that were perfused with 4% paraformaldehyde. I
found protocols for FF, but not fixed frozen. I tried staining them with my
usual Gomori I use on my paraffin blocks but the doc thinks they picked up too
much red, not enough green.
Any suggerstions..I need to g
It's a do-it-yourself.
- Scroll to bottom of any Histonet email.
- Click on the link that has the word "lists" in it.
- Scroll to the bottom of that page, where there is information on how to
unsubscribe.
- Follow directions.
Peggy Wenki
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